Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay

Caiman latirostris is one of the two crocodilian species that inhabit Argentina. In this country, as a consequence of agricultural frontiers expansion during the last years, many areas of the geographic distribution of the broad snouted caiman overlap with regions of intensive agricultural activity....

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Autores principales: Poletta, G.L., Larriera, A., Kleinsorge, E., Mudry, M.D.
Formato: JOUR
Materias:
Caiman latirostris
Comet assay
Genotoxicity
Micronuclei
Sentinel organism
article
Chordata
comet assay
controlled study
genotoxicity
micronucleus
nonhuman
pollutant
pollution
priority journal
Alligators and Crocodiles
Animals
Comet Assay
DNA Damage
Environmental Monitoring
Female
Male
Micronucleus Tests
Animalia
Caiman
Crocodylidae (all crocodiles)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_13835718_v650_n2_p202_Poletta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_13835718_v650_n2_p202_Poletta
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spelling todo:paper_13835718_v650_n2_p202_Poletta2023-10-03T16:11:55Z Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay Poletta, G.L. Larriera, A. Kleinsorge, E. Mudry, M.D. Caiman latirostris Comet assay Genotoxicity Micronuclei Sentinel organism article Caiman latirostris Chordata comet assay controlled study genotoxicity micronucleus nonhuman pollutant pollution priority journal Alligators and Crocodiles Animals Comet Assay DNA Damage Environmental Monitoring Female Male Micronucleus Tests Animalia Caiman Caiman latirostris Crocodylidae (all crocodiles) Caiman latirostris is one of the two crocodilian species that inhabit Argentina. In this country, as a consequence of agricultural frontiers expansion during the last years, many areas of the geographic distribution of the broad snouted caiman overlap with regions of intensive agricultural activity. Contaminants released to the environment may induce genetic alterations in wildlife, which could lead to mutations and/or carcinogenesis. Up to the moment, no studies had been made concerning the possibbility to apply biomarkers of genotoxic evaluation in C. latirostris. The aim of this study was to adapt two widely used genotoxic techniques, the comet assay and the micronucleus test, for their application in C. latirostris and to determine the baseline values in this species, in order to establish its suitability as a sentinel organism for future genotoxic monitoring of environmental pollutants. A total of 41 juvenile caimans of 4 months old (FMO) and 10 months old (TMO) were used. Genotoxic techniques were applied on peripheral blood erythrocytes introducing the necessary modifications required by the material, which are presented here. Our results show that baseline values of DNA damage are quite stable among juvenile caimans (MN: FMO animals 0.87 ± 0.74 and TMO animals 1.04 ± 0.92; DI: FMO animals 103.40 ± 3.36 and TMO animals 120.08 ± 11.33), being independent of the nest of origin, sex and size of the animals and confirm the potential value of both short term tests as accurate screening tools for the evaluation of genotoxic agents in C. latirostris. This is the first reference to the application of genotoxic techniques on C. latirostris and the second in crocodilians. Data provided here will be useful for future studies involving the biomonitoring of natural regions where C. latirostris occurs, employing this species as a sentinel organism for genotoxic assessment of environmental pollutants. © 2007 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13835718_v650_n2_p202_Poletta
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Caiman latirostris
Comet assay
Genotoxicity
Micronuclei
Sentinel organism
article
Caiman latirostris
Chordata
comet assay
controlled study
genotoxicity
micronucleus
nonhuman
pollutant
pollution
priority journal
Alligators and Crocodiles
Animals
Comet Assay
DNA Damage
Environmental Monitoring
Female
Male
Micronucleus Tests
Animalia
Caiman
Caiman latirostris
Crocodylidae (all crocodiles)
spellingShingle Caiman latirostris
Comet assay
Genotoxicity
Micronuclei
Sentinel organism
article
Caiman latirostris
Chordata
comet assay
controlled study
genotoxicity
micronucleus
nonhuman
pollutant
pollution
priority journal
Alligators and Crocodiles
Animals
Comet Assay
DNA Damage
Environmental Monitoring
Female
Male
Micronucleus Tests
Animalia
Caiman
Caiman latirostris
Crocodylidae (all crocodiles)
Poletta, G.L.
Larriera, A.
Kleinsorge, E.
Mudry, M.D.
Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay
topic_facet Caiman latirostris
Comet assay
Genotoxicity
Micronuclei
Sentinel organism
article
Caiman latirostris
Chordata
comet assay
controlled study
genotoxicity
micronucleus
nonhuman
pollutant
pollution
priority journal
Alligators and Crocodiles
Animals
Comet Assay
DNA Damage
Environmental Monitoring
Female
Male
Micronucleus Tests
Animalia
Caiman
Caiman latirostris
Crocodylidae (all crocodiles)
description Caiman latirostris is one of the two crocodilian species that inhabit Argentina. In this country, as a consequence of agricultural frontiers expansion during the last years, many areas of the geographic distribution of the broad snouted caiman overlap with regions of intensive agricultural activity. Contaminants released to the environment may induce genetic alterations in wildlife, which could lead to mutations and/or carcinogenesis. Up to the moment, no studies had been made concerning the possibbility to apply biomarkers of genotoxic evaluation in C. latirostris. The aim of this study was to adapt two widely used genotoxic techniques, the comet assay and the micronucleus test, for their application in C. latirostris and to determine the baseline values in this species, in order to establish its suitability as a sentinel organism for future genotoxic monitoring of environmental pollutants. A total of 41 juvenile caimans of 4 months old (FMO) and 10 months old (TMO) were used. Genotoxic techniques were applied on peripheral blood erythrocytes introducing the necessary modifications required by the material, which are presented here. Our results show that baseline values of DNA damage are quite stable among juvenile caimans (MN: FMO animals 0.87 ± 0.74 and TMO animals 1.04 ± 0.92; DI: FMO animals 103.40 ± 3.36 and TMO animals 120.08 ± 11.33), being independent of the nest of origin, sex and size of the animals and confirm the potential value of both short term tests as accurate screening tools for the evaluation of genotoxic agents in C. latirostris. This is the first reference to the application of genotoxic techniques on C. latirostris and the second in crocodilians. Data provided here will be useful for future studies involving the biomonitoring of natural regions where C. latirostris occurs, employing this species as a sentinel organism for genotoxic assessment of environmental pollutants. © 2007 Elsevier B.V. All rights reserved.
format JOUR
author Poletta, G.L.
Larriera, A.
Kleinsorge, E.
Mudry, M.D.
author_facet Poletta, G.L.
Larriera, A.
Kleinsorge, E.
Mudry, M.D.
author_sort Poletta, G.L.
title Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay
title_short Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay
title_full Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay
title_fullStr Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay
title_full_unstemmed Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay
title_sort caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: basal values determination of micronucleus and comet assay
url http://hdl.handle.net/20.500.12110/paper_13835718_v650_n2_p202_Poletta
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AT kleinsorgee caimanlatirostrisbroadsnoutedcaimanasasentinelorganismforgenotoxicmonitoringbasalvaluesdeterminationofmicronucleusandcometassay
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