Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers

Tetraploid Paspalum notatum (bahiagrass) is a valuable forage grass with aposporous apomictic reproduction. In a previous study, we showed that apospory in bahiagrass is under the control of a single dominant gene with a distorted segregation ratio. The objective of this work was to identify molecul...

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Autores principales: Martínez, E.J., Hopp, H.E., Stein, J., Ortiz, J.P.A., Quarin, C.L.
Formato: JOUR
Materias:
Apomixis
Apospory
Bulked segregant analysis
Comparative mapping
Molecular markers
Paspalum notatum
Embryophyta
Paspalum
Paspalum simplex
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_13803743_v12_n4_p319_Martinez
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_13803743_v12_n4_p319_Martinez2023-10-03T16:11:40Z Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers Martínez, E.J. Hopp, H.E. Stein, J. Ortiz, J.P.A. Quarin, C.L. Apomixis Apospory Bulked segregant analysis Comparative mapping Molecular markers Paspalum notatum Embryophyta Paspalum Paspalum notatum Paspalum simplex Tetraploid Paspalum notatum (bahiagrass) is a valuable forage grass with aposporous apomictic reproduction. In a previous study, we showed that apospory in bahiagrass is under the control of a single dominant gene with a distorted segregation ratio. The objective of this work was to identify molecular markers linked to apospory in tetraploid P. notatum and establish a preliminary syntenic relationship with the genomic region associated with apospory in P. simplex. A F1 population of 290 individuals, segregating for apospory, was generated after crossing a completely sexual plant (Q4188) with a natural aposporous apomictic plant (Q4117). The whole progeny was classified as sexual or aposporous by embryo sacs analysis. A bulked segregant analysis was carried out to identify molecular markers co-segregating with apospory. Four hundred RAPD primers, 30 AFLP primers combinations and 85 RFLP clones were screened using DNA from both parental genotypes and aposporous and sexual bulks. Linkage analysis was performed with cytological and genetic information from the complete progeny. Cytoembryological analysis showed 219 sexual and 71 aposporous F1 individuals. Seven different molecular markers (2 RAPD, 4 AFLP and 1 RFLP) were found to be completely linked to apospory. The RFLP probe C1069, mapping to the telomeric region of the long arm of rice chromosome 12, was one of the molecular markers completely linked to apospory in P. notatum. This marker had been previously associated with apospory in P. simplex. A preliminary map of the chromosome region carrying the apospory locus was constructed. Fil:Martínez, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13803743_v12_n4_p319_Martinez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Apomixis
Apospory
Bulked segregant analysis
Comparative mapping
Molecular markers
Paspalum notatum
Embryophyta
Paspalum
Paspalum notatum
Paspalum simplex
spellingShingle Apomixis
Apospory
Bulked segregant analysis
Comparative mapping
Molecular markers
Paspalum notatum
Embryophyta
Paspalum
Paspalum notatum
Paspalum simplex
Martínez, E.J.
Hopp, H.E.
Stein, J.
Ortiz, J.P.A.
Quarin, C.L.
Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
topic_facet Apomixis
Apospory
Bulked segregant analysis
Comparative mapping
Molecular markers
Paspalum notatum
Embryophyta
Paspalum
Paspalum notatum
Paspalum simplex
description Tetraploid Paspalum notatum (bahiagrass) is a valuable forage grass with aposporous apomictic reproduction. In a previous study, we showed that apospory in bahiagrass is under the control of a single dominant gene with a distorted segregation ratio. The objective of this work was to identify molecular markers linked to apospory in tetraploid P. notatum and establish a preliminary syntenic relationship with the genomic region associated with apospory in P. simplex. A F1 population of 290 individuals, segregating for apospory, was generated after crossing a completely sexual plant (Q4188) with a natural aposporous apomictic plant (Q4117). The whole progeny was classified as sexual or aposporous by embryo sacs analysis. A bulked segregant analysis was carried out to identify molecular markers co-segregating with apospory. Four hundred RAPD primers, 30 AFLP primers combinations and 85 RFLP clones were screened using DNA from both parental genotypes and aposporous and sexual bulks. Linkage analysis was performed with cytological and genetic information from the complete progeny. Cytoembryological analysis showed 219 sexual and 71 aposporous F1 individuals. Seven different molecular markers (2 RAPD, 4 AFLP and 1 RFLP) were found to be completely linked to apospory. The RFLP probe C1069, mapping to the telomeric region of the long arm of rice chromosome 12, was one of the molecular markers completely linked to apospory in P. notatum. This marker had been previously associated with apospory in P. simplex. A preliminary map of the chromosome region carrying the apospory locus was constructed.
format JOUR
author Martínez, E.J.
Hopp, H.E.
Stein, J.
Ortiz, J.P.A.
Quarin, C.L.
author_facet Martínez, E.J.
Hopp, H.E.
Stein, J.
Ortiz, J.P.A.
Quarin, C.L.
author_sort Martínez, E.J.
title Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_short Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_full Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_fullStr Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_full_unstemmed Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_sort genetic characterization of apospory in tetraploid paspalum notatum based on the identification of linked molecular markers
url http://hdl.handle.net/20.500.12110/paper_13803743_v12_n4_p319_Martinez
work_keys_str_mv AT martinezej geneticcharacterizationofaposporyintetraploidpaspalumnotatumbasedontheidentificationoflinkedmolecularmarkers
AT hopphe geneticcharacterizationofaposporyintetraploidpaspalumnotatumbasedontheidentificationoflinkedmolecularmarkers
AT steinj geneticcharacterizationofaposporyintetraploidpaspalumnotatumbasedontheidentificationoflinkedmolecularmarkers
AT ortizjpa geneticcharacterizationofaposporyintetraploidpaspalumnotatumbasedontheidentificationoflinkedmolecularmarkers
AT quarincl geneticcharacterizationofaposporyintetraploidpaspalumnotatumbasedontheidentificationoflinkedmolecularmarkers
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