Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila

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High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophil...

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Autores principales:	De Panis, D.N., Padró, J., Furió-Tarí, P., Tarazona, S., Milla Carmona, P.S., Soto, I.M., Dopazo, H., Conesa, A., Hasson, E.
Formato:	JOUR
Materias:	alkaloids environment adaptation mescaline plasticity RNA-Seq alkaloid transcriptome adaptation animal Argentina Cactaceae chemistry desert climate Drosophila genetics larva Adaptation, Physiological Alkaloids Animals Desert Climate Larva Transcriptome
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1365294X_v25_n18_p4534_DePanis
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1365294X_v25_n18_p4534_DePanis
record_format	dspace
spelling	todo:paper_1365294X_v25_n18_p4534_DePanis2023-10-03T16:11:19Z Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila De Panis, D.N. Padró, J. Furió-Tarí, P. Tarazona, S. Milla Carmona, P.S. Soto, I.M. Dopazo, H. Conesa, A. Hasson, E. alkaloids environment adaptation mescaline plasticity RNA-Seq alkaloid transcriptome adaptation animal Argentina Cactaceae chemistry desert climate Drosophila genetics larva Adaptation, Physiological Alkaloids Animals Argentina Cactaceae Desert Climate Drosophila Larva Transcriptome High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation-reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments. © 2016 John Wiley & Sons Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1365294X_v25_n18_p4534_DePanis
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	alkaloids environment adaptation mescaline plasticity RNA-Seq alkaloid transcriptome adaptation animal Argentina Cactaceae chemistry desert climate Drosophila genetics larva Adaptation, Physiological Alkaloids Animals Argentina Cactaceae Desert Climate Drosophila Larva Transcriptome
spellingShingle	alkaloids environment adaptation mescaline plasticity RNA-Seq alkaloid transcriptome adaptation animal Argentina Cactaceae chemistry desert climate Drosophila genetics larva Adaptation, Physiological Alkaloids Animals Argentina Cactaceae Desert Climate Drosophila Larva Transcriptome De Panis, D.N. Padró, J. Furió-Tarí, P. Tarazona, S. Milla Carmona, P.S. Soto, I.M. Dopazo, H. Conesa, A. Hasson, E. Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
topic_facet	alkaloids environment adaptation mescaline plasticity RNA-Seq alkaloid transcriptome adaptation animal Argentina Cactaceae chemistry desert climate Drosophila genetics larva Adaptation, Physiological Alkaloids Animals Argentina Cactaceae Desert Climate Drosophila Larva Transcriptome
description	High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation-reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments. © 2016 John Wiley & Sons Ltd.
format	JOUR
author	De Panis, D.N. Padró, J. Furió-Tarí, P. Tarazona, S. Milla Carmona, P.S. Soto, I.M. Dopazo, H. Conesa, A. Hasson, E.
author_facet	De Panis, D.N. Padró, J. Furió-Tarí, P. Tarazona, S. Milla Carmona, P.S. Soto, I.M. Dopazo, H. Conesa, A. Hasson, E.
author_sort	De Panis, D.N.
title	Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_short	Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_full	Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_fullStr	Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_full_unstemmed	Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_sort	transcriptome modulation during host shift is driven by secondary metabolites in desert drosophila
url	http://hdl.handle.net/20.500.12110/paper_1365294X_v25_n18_p4534_DePanis
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_version_	1807315887528607744

Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila

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