Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin

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Aims: In this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. Methods and Results: We determined, using a virus yield inh...

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Autores principales:	Quintana, V.M., Torres, N.I., Wachsman, M.B., Sinko, P.J., Castilla, V., Chikindas, M.
Formato:	INPR
Materias:	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13645072_v_n_p_Quintana
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_13645072_v_n_p_Quintana
record_format	dspace
spelling	todo:paper_13645072_v_n_p_Quintana2023-10-03T16:11:06Z Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin Quintana, V.M. Torres, N.I. Wachsman, M.B. Sinko, P.J. Castilla, V. Chikindas, M. Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin Aims: In this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. Methods and Results: We determined, using a virus yield inhibition assay, that noncytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h postinfection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin-treated culture. On the other hand, at high concentrations, subtilosin displays virucidal action. Conclusions: Subtilosin displays antiviral and virucidal actions against HSV-2. The target of subtilosin inhibitory effect would be late stages of the viral replicative cycle such as viral glycoprotein intracellular transport. Significance and Impact of the Study: Given its antimicrobial activity and its safety for human tissues, subtilosin could represent a valuable alternative to be considered in the development of new microbicide formulations. © 2014 The Society for Applied Microbiology. Fil:Torres, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. INPR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13645072_v_n_p_Quintana
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin
spellingShingle	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin Quintana, V.M. Torres, N.I. Wachsman, M.B. Sinko, P.J. Castilla, V. Chikindas, M. Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
topic_facet	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin
description	Aims: In this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. Methods and Results: We determined, using a virus yield inhibition assay, that noncytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h postinfection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin-treated culture. On the other hand, at high concentrations, subtilosin displays virucidal action. Conclusions: Subtilosin displays antiviral and virucidal actions against HSV-2. The target of subtilosin inhibitory effect would be late stages of the viral replicative cycle such as viral glycoprotein intracellular transport. Significance and Impact of the Study: Given its antimicrobial activity and its safety for human tissues, subtilosin could represent a valuable alternative to be considered in the development of new microbicide formulations. © 2014 The Society for Applied Microbiology.
format	INPR
author	Quintana, V.M. Torres, N.I. Wachsman, M.B. Sinko, P.J. Castilla, V. Chikindas, M.
author_facet	Quintana, V.M. Torres, N.I. Wachsman, M.B. Sinko, P.J. Castilla, V. Chikindas, M.
author_sort	Quintana, V.M.
title	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_short	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_full	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_fullStr	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_full_unstemmed	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_sort	antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
url	http://hdl.handle.net/20.500.12110/paper_13645072_v_n_p_Quintana
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_version_	1807321683074220032

Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin

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