Occurrence of GABA and GABA receptors in human spermatozoa

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Gamma-aminobutyric acid (GABA) concentrations in seminal plasma and washed spermatozoa from normal donors were assessed by a sensitive radioreceptor assay, and were detectable in both fractions. Specific binding of [3H]-muscimol was shown to be dependent on protein concentration, temperature and inc...

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Autores principales:	Ritta, M.N., Calamera, J.C., Bas, D.E.
Formato:	JOUR
Materias:	GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13609947_v4_n8_p769_Ritta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_13609947_v4_n8_p769_Ritta
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spelling	todo:paper_13609947_v4_n8_p769_Ritta2023-10-03T16:10:52Z Occurrence of GABA and GABA receptors in human spermatozoa Ritta, M.N. Calamera, J.C. Bas, D.E. GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility Gamma-aminobutyric acid (GABA) concentrations in seminal plasma and washed spermatozoa from normal donors were assessed by a sensitive radioreceptor assay, and were detectable in both fractions. Specific binding of [3H]-muscimol was shown to be dependent on protein concentration, temperature and incubation time. [3H]-muscimol specific binding to human sperm membranes was significantly inhibited by the GABA type A receptor (GABA(A)) antagonist, bicuculline, and by the GABA(A) agonists, muscimol and isoguvacine, but not by the GABA type B receptor (GABA(B)) agonist baclofen. Scatchard analysis of [3H]-muscimol binding yielded a linear plot consistent with a single population of binding sites with a dissociation constant in the low nanomolar range. Incubation with GABA at a high micromolar concentration for 3 h under capacitating conditions resulted in an increase in the percentage of spermatozoa showing hyperactivated motility as assessed by computerized motility analyser. However, low micromolar concentrations of the GABA(A) agonist, muscimol, were sufficient to significantly increase sperm hyperactivity. These results suggest that the effect of GABA on human sperm motility might be mediated through a specific GABA(A) receptor. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13609947_v4_n8_p769_Ritta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility
spellingShingle	GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility Ritta, M.N. Calamera, J.C. Bas, D.E. Occurrence of GABA and GABA receptors in human spermatozoa
topic_facet	GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility
description	Gamma-aminobutyric acid (GABA) concentrations in seminal plasma and washed spermatozoa from normal donors were assessed by a sensitive radioreceptor assay, and were detectable in both fractions. Specific binding of [3H]-muscimol was shown to be dependent on protein concentration, temperature and incubation time. [3H]-muscimol specific binding to human sperm membranes was significantly inhibited by the GABA type A receptor (GABA(A)) antagonist, bicuculline, and by the GABA(A) agonists, muscimol and isoguvacine, but not by the GABA type B receptor (GABA(B)) agonist baclofen. Scatchard analysis of [3H]-muscimol binding yielded a linear plot consistent with a single population of binding sites with a dissociation constant in the low nanomolar range. Incubation with GABA at a high micromolar concentration for 3 h under capacitating conditions resulted in an increase in the percentage of spermatozoa showing hyperactivated motility as assessed by computerized motility analyser. However, low micromolar concentrations of the GABA(A) agonist, muscimol, were sufficient to significantly increase sperm hyperactivity. These results suggest that the effect of GABA on human sperm motility might be mediated through a specific GABA(A) receptor.
format	JOUR
author	Ritta, M.N. Calamera, J.C. Bas, D.E.
author_facet	Ritta, M.N. Calamera, J.C. Bas, D.E.
author_sort	Ritta, M.N.
title	Occurrence of GABA and GABA receptors in human spermatozoa
title_short	Occurrence of GABA and GABA receptors in human spermatozoa
title_full	Occurrence of GABA and GABA receptors in human spermatozoa
title_fullStr	Occurrence of GABA and GABA receptors in human spermatozoa
title_full_unstemmed	Occurrence of GABA and GABA receptors in human spermatozoa
title_sort	occurrence of gaba and gaba receptors in human spermatozoa
url	http://hdl.handle.net/20.500.12110/paper_13609947_v4_n8_p769_Ritta
work_keys_str_mv	AT rittamn occurrenceofgabaandgabareceptorsinhumanspermatozoa AT calamerajc occurrenceofgabaandgabareceptorsinhumanspermatozoa AT basde occurrenceofgabaandgabareceptorsinhumanspermatozoa
_version_	1807322538576969728

Occurrence of GABA and GABA receptors in human spermatozoa

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