Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats

The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Iso...

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Autores principales: Andreu, C.V., Buscaglia, C.A., Parborell, F., Stein, P., Tesone, M.
Formato: JOUR
Materias:
rat
DNA
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p145_Andreu
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spelling todo:paper_1355008X_v6_n2_p145_Andreu2023-10-03T16:10:16Z Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats Andreu, C.V. Buscaglia, C.A. Parborell, F. Stein, P. Tesone, M. Corpus luteum Follicle differentiation Granulosa cell Ovary chorionic gonadotropin estradiol follitropin gonadotropin luteinizing hormone novormon seric gonadotropin thymidine unclassified drug animal cell animal experiment article controlled study corpus luteum female granulosa cell luteal cell nonhuman ovary follicle priority journal rat Animals Cell Differentiation Cells, Cultured Corpus Luteum Culture Media, Conditioned DNA Female Gonadotropins Gonadotropins, Equine Granulosa Cells Ovarian Follicle Ovary Pregnancy Rats Rats, Sprague-Dawley Steroids Stimulation, Chemical Thymidine The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Isolated granulosa cells were cultured in serum-free medium, different stimuli added for periods of 48 h, and 3H-thymidine incorporation was measured. Both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) inhibited 3H-thymidine incorporation by cultured granulosa cells in a dose-dependent manner (FSH: 10, 100, 200 ng/mL = 26, 41, 49% inhibition, respectively; LH: 0.1, 1, 10 ng/mL = 11, 37, 75% inhibition, respectively). On the other hand, estradiol was found to stimulate 3H-thymidine incorporation in granulosa cells (Estradiol: 5, 50, 500 ng/mL = 17, 37, 76% stimulation, respectively). In luteal cells, the rate of basal 3H-thymidine incorporation was very low (granulosa cells: 2560 ± 310; luteal cells: 661 ± 92 cpm/100,000 cells) and not modified by any stimulus. To determine the possible production of an inhibitory growth factor by the early corpus luteum, 3H-thymidine incorporation by granulosa cells was assessed in the presence of 10% conditioned media (CM) recovered from luteal cell cultures. A marked inhibition both in basal and estradiol-stimulated 3H-thymidine incorporation was observed (74 and 76% of inhibition, respectively). Results suggest that an inhibitory growth factor produced by luteal cells after luteinizing gonadotrophin stimulus could be involved in the differentiation of growing follicles to corpus luteum. Fil:Andreu, C.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buscaglia, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stein, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p145_Andreu
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Corpus luteum
Follicle differentiation
Granulosa cell
Ovary
chorionic gonadotropin
estradiol
follitropin
gonadotropin
luteinizing hormone
novormon
seric gonadotropin
thymidine
unclassified drug
animal cell
animal experiment
article
controlled study
corpus luteum
female
granulosa cell
luteal cell
nonhuman
ovary follicle
priority journal
rat
Animals
Cell Differentiation
Cells, Cultured
Corpus Luteum
Culture Media, Conditioned
DNA
Female
Gonadotropins
Gonadotropins, Equine
Granulosa Cells
Ovarian Follicle
Ovary
Pregnancy
Rats
Rats, Sprague-Dawley
Steroids
Stimulation, Chemical
Thymidine
spellingShingle Corpus luteum
Follicle differentiation
Granulosa cell
Ovary
chorionic gonadotropin
estradiol
follitropin
gonadotropin
luteinizing hormone
novormon
seric gonadotropin
thymidine
unclassified drug
animal cell
animal experiment
article
controlled study
corpus luteum
female
granulosa cell
luteal cell
nonhuman
ovary follicle
priority journal
rat
Animals
Cell Differentiation
Cells, Cultured
Corpus Luteum
Culture Media, Conditioned
DNA
Female
Gonadotropins
Gonadotropins, Equine
Granulosa Cells
Ovarian Follicle
Ovary
Pregnancy
Rats
Rats, Sprague-Dawley
Steroids
Stimulation, Chemical
Thymidine
Andreu, C.V.
Buscaglia, C.A.
Parborell, F.
Stein, P.
Tesone, M.
Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
topic_facet Corpus luteum
Follicle differentiation
Granulosa cell
Ovary
chorionic gonadotropin
estradiol
follitropin
gonadotropin
luteinizing hormone
novormon
seric gonadotropin
thymidine
unclassified drug
animal cell
animal experiment
article
controlled study
corpus luteum
female
granulosa cell
luteal cell
nonhuman
ovary follicle
priority journal
rat
Animals
Cell Differentiation
Cells, Cultured
Corpus Luteum
Culture Media, Conditioned
DNA
Female
Gonadotropins
Gonadotropins, Equine
Granulosa Cells
Ovarian Follicle
Ovary
Pregnancy
Rats
Rats, Sprague-Dawley
Steroids
Stimulation, Chemical
Thymidine
description The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Isolated granulosa cells were cultured in serum-free medium, different stimuli added for periods of 48 h, and 3H-thymidine incorporation was measured. Both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) inhibited 3H-thymidine incorporation by cultured granulosa cells in a dose-dependent manner (FSH: 10, 100, 200 ng/mL = 26, 41, 49% inhibition, respectively; LH: 0.1, 1, 10 ng/mL = 11, 37, 75% inhibition, respectively). On the other hand, estradiol was found to stimulate 3H-thymidine incorporation in granulosa cells (Estradiol: 5, 50, 500 ng/mL = 17, 37, 76% stimulation, respectively). In luteal cells, the rate of basal 3H-thymidine incorporation was very low (granulosa cells: 2560 ± 310; luteal cells: 661 ± 92 cpm/100,000 cells) and not modified by any stimulus. To determine the possible production of an inhibitory growth factor by the early corpus luteum, 3H-thymidine incorporation by granulosa cells was assessed in the presence of 10% conditioned media (CM) recovered from luteal cell cultures. A marked inhibition both in basal and estradiol-stimulated 3H-thymidine incorporation was observed (74 and 76% of inhibition, respectively). Results suggest that an inhibitory growth factor produced by luteal cells after luteinizing gonadotrophin stimulus could be involved in the differentiation of growing follicles to corpus luteum.
format JOUR
author Andreu, C.V.
Buscaglia, C.A.
Parborell, F.
Stein, P.
Tesone, M.
author_facet Andreu, C.V.
Buscaglia, C.A.
Parborell, F.
Stein, P.
Tesone, M.
author_sort Andreu, C.V.
title Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
title_short Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
title_full Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
title_fullStr Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
title_full_unstemmed Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
title_sort regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
url http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p145_Andreu
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