N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAO...
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todo:paper_13510002_v22_n6_p265_Soto2023-10-03T16:10:06Z N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation Soto, D. Gomez-Serrano, M. Pieralisi, A. Calvo, J.C. Peral, B. Guerra, L.N. 3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210–218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation. © 2016 Informa UK Limited, trading as Taylor & Francis Group. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13510002_v22_n6_p265_Soto |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation |
| spellingShingle |
3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation Soto, D. Gomez-Serrano, M. Pieralisi, A. Calvo, J.C. Peral, B. Guerra, L.N. N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation |
| topic_facet |
3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation |
| description |
Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210–218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation. © 2016 Informa UK Limited, trading as Taylor & Francis Group. |
| format |
JOUR |
| author |
Soto, D. Gomez-Serrano, M. Pieralisi, A. Calvo, J.C. Peral, B. Guerra, L.N. |
| author_facet |
Soto, D. Gomez-Serrano, M. Pieralisi, A. Calvo, J.C. Peral, B. Guerra, L.N. |
| author_sort |
Soto, D. |
| title |
N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation |
| title_short |
N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation |
| title_full |
N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation |
| title_fullStr |
N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation |
| title_full_unstemmed |
N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation |
| title_sort |
n-acetylcysteine inhibits kinase phosphorylation during 3t3-l1 adipocyte differentiation |
| url |
http://hdl.handle.net/20.500.12110/paper_13510002_v22_n6_p265_Soto |
| work_keys_str_mv |
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| _version_ |
1807318693405786112 |