Thyroid hormone effect in human hepatocytes
We have already demonstrated that a combined treatment of methimazole and an antioxidant mixture improved the condition of hyperthyroid patients both biochemically and clinically. Elevated thyroid hormone levels might trigger signs and symptoms of hyperthyroidism through the increase of free radical...
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todo:paper_13510002_v13_n4_p185_Miler2023-10-03T16:10:05Z Thyroid hormone effect in human hepatocytes Miler, E.A. Ríos de Molina, M. del C. Domínguez, G. Guerra, L.N. Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine We have already demonstrated that a combined treatment of methimazole and an antioxidant mixture improved the condition of hyperthyroid patients both biochemically and clinically. Elevated thyroid hormone levels might trigger signs and symptoms of hyperthyroidism through the increase of free radicals. To study the direct effect of thyroid hormone on cellular markers of oxidative stress, we carried out in vitro assays in which 0.1-20.0 nM T3 (6.5-1300.0 ng/dl) doses were added to culture media of the human hepatocyte cell line Hep G2 for 1-24 h. T3 increased malondialdehyde (MDA) and intracellular oxidized glutathione (GSSG) levels; SOD activity was also higher with hormone treatment, whereas catalase and glutathione peroxidase activities showed no variation at different T3 doses and during all experimental times. When ascorbic acid was added to the culture, the MDA level decreased and SOD activity was increased. With higher doses of T3 (e.g. 200 nM), cell death occurred (69% of apoptotic cells). The increase in SOD activity was not enough to overcome the effect of T3 since MDA and GSSG remained high during a 24-h experiment. We showed a beneficial effect of ascorbic acid when cells were exposed to a T3 dose of 20 nM, a higher level of hormone than that achieved in hyperthyroidism. © 2008 W. S. Maney and Son Ltd. Fil:Miler, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos de Molina, M. del C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13510002_v13_n4_p185_Miler |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine |
| spellingShingle |
Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine Miler, E.A. Ríos de Molina, M. del C. Domínguez, G. Guerra, L.N. Thyroid hormone effect in human hepatocytes |
| topic_facet |
Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine |
| description |
We have already demonstrated that a combined treatment of methimazole and an antioxidant mixture improved the condition of hyperthyroid patients both biochemically and clinically. Elevated thyroid hormone levels might trigger signs and symptoms of hyperthyroidism through the increase of free radicals. To study the direct effect of thyroid hormone on cellular markers of oxidative stress, we carried out in vitro assays in which 0.1-20.0 nM T3 (6.5-1300.0 ng/dl) doses were added to culture media of the human hepatocyte cell line Hep G2 for 1-24 h. T3 increased malondialdehyde (MDA) and intracellular oxidized glutathione (GSSG) levels; SOD activity was also higher with hormone treatment, whereas catalase and glutathione peroxidase activities showed no variation at different T3 doses and during all experimental times. When ascorbic acid was added to the culture, the MDA level decreased and SOD activity was increased. With higher doses of T3 (e.g. 200 nM), cell death occurred (69% of apoptotic cells). The increase in SOD activity was not enough to overcome the effect of T3 since MDA and GSSG remained high during a 24-h experiment. We showed a beneficial effect of ascorbic acid when cells were exposed to a T3 dose of 20 nM, a higher level of hormone than that achieved in hyperthyroidism. © 2008 W. S. Maney and Son Ltd. |
| format |
JOUR |
| author |
Miler, E.A. Ríos de Molina, M. del C. Domínguez, G. Guerra, L.N. |
| author_facet |
Miler, E.A. Ríos de Molina, M. del C. Domínguez, G. Guerra, L.N. |
| author_sort |
Miler, E.A. |
| title |
Thyroid hormone effect in human hepatocytes |
| title_short |
Thyroid hormone effect in human hepatocytes |
| title_full |
Thyroid hormone effect in human hepatocytes |
| title_fullStr |
Thyroid hormone effect in human hepatocytes |
| title_full_unstemmed |
Thyroid hormone effect in human hepatocytes |
| title_sort |
thyroid hormone effect in human hepatocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_13510002_v13_n4_p185_Miler |
| work_keys_str_mv |
AT milerea thyroidhormoneeffectinhumanhepatocytes AT riosdemolinamdelc thyroidhormoneeffectinhumanhepatocytes AT dominguezg thyroidhormoneeffectinhumanhepatocytes AT guerraln thyroidhormoneeffectinhumanhepatocytes |
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1782025792989954048 |