Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid
The probiotic Gram-positive bacterium Lactobacillus casei BL23 is naturally confronted with salt-stress habitats. It has been previously reported that growth in high-salt medium, containing 0.8 M NaCl, leads to modifications in the cell envelope of this bacterium. In this study, we report that L. ca...
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todo:paper_13500872_v159_nPART11_p2416_Palomino2023-10-03T16:09:54Z Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid Palomino, M.M. Allievi, M.C. Gründling, A. Sanchez-Rivas, C. Ruzal, S.M. bacterial enzyme cation dextro alanine lipoteichoic acid messenger RNA phosphatidylglycerol sodium chloride teichoic acid adaptation article bacterial cell wall bacterial growth binding affinity biofilm cloning controlled study enzyme activity gene expression hydrolysis in vitro study Lactobacillus casei nonhuman operon osmotic stress priority journal protein purification reduction salt stress substitution reaction surface property Adaptation, Physiological Biofilms Cations Cell Wall Culture Media Gene Expression Profiling Lactobacillus casei Lipopolysaccharides Osmotic Pressure Protein Binding Teichoic Acids The probiotic Gram-positive bacterium Lactobacillus casei BL23 is naturally confronted with salt-stress habitats. It has been previously reported that growth in high-salt medium, containing 0.8 M NaCl, leads to modifications in the cell envelope of this bacterium. In this study, we report that L. casei BL23 has an increased ability to form biofilms and to bind cations in high-salt conditions. This behaviour correlated with modifications of surface properties involving teichoic acids, which are important cell wall components. We also showed that, in these high-salt conditions, L. casei BL23 produces less of the cell wall polymer lipoteichoic acid (LTA), and that this anionic polymer has a shorter mean chain length and a lower level of D-alanyl-substitution. Analysis of the transcript levels of the dltABCD operon, encoding the enzymes required for the incorporation of D-alanine into anionic polymers, showed a 16-fold reduction in mRNA levels, which is consistent with a decrease in D-alanine substitutions on LTA. Furthermore, a 13-fold reduction in the transcript levels was observed for the gene LCABL_09330 coding for a putative LTA synthase. To provide further experimental evidence that LCABL_09330 is a true LTA synthase (LtaS) in L. casei BL23, the enzymic domain was cloned and expressed in E. coli. The purified protein was able to hydrolyse the membrane lipid phosphatidylglycerol as expected for an LTA synthase enzyme, and hence LCABL_09330 was renamed LtaS. The purified enzyme showed Mn2+-ion dependent activity, and its activity was modulated by differences in NaCl concentration. The decrease in both ltaS transcript levels and enzyme activity observed in high-salt conditions might influence the length of the LTA backbone chain. A putative function of the modified LTA structure is discussed that is compatible with the growth under salt-stress conditions and with the overall envelope modifications taking place during this stress condition. © 2013 SGM. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13500872_v159_nPART11_p2416_Palomino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial enzyme cation dextro alanine lipoteichoic acid messenger RNA phosphatidylglycerol sodium chloride teichoic acid adaptation article bacterial cell wall bacterial growth binding affinity biofilm cloning controlled study enzyme activity gene expression hydrolysis in vitro study Lactobacillus casei nonhuman operon osmotic stress priority journal protein purification reduction salt stress substitution reaction surface property Adaptation, Physiological Biofilms Cations Cell Wall Culture Media Gene Expression Profiling Lactobacillus casei Lipopolysaccharides Osmotic Pressure Protein Binding Teichoic Acids |
| spellingShingle |
bacterial enzyme cation dextro alanine lipoteichoic acid messenger RNA phosphatidylglycerol sodium chloride teichoic acid adaptation article bacterial cell wall bacterial growth binding affinity biofilm cloning controlled study enzyme activity gene expression hydrolysis in vitro study Lactobacillus casei nonhuman operon osmotic stress priority journal protein purification reduction salt stress substitution reaction surface property Adaptation, Physiological Biofilms Cations Cell Wall Culture Media Gene Expression Profiling Lactobacillus casei Lipopolysaccharides Osmotic Pressure Protein Binding Teichoic Acids Palomino, M.M. Allievi, M.C. Gründling, A. Sanchez-Rivas, C. Ruzal, S.M. Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid |
| topic_facet |
bacterial enzyme cation dextro alanine lipoteichoic acid messenger RNA phosphatidylglycerol sodium chloride teichoic acid adaptation article bacterial cell wall bacterial growth binding affinity biofilm cloning controlled study enzyme activity gene expression hydrolysis in vitro study Lactobacillus casei nonhuman operon osmotic stress priority journal protein purification reduction salt stress substitution reaction surface property Adaptation, Physiological Biofilms Cations Cell Wall Culture Media Gene Expression Profiling Lactobacillus casei Lipopolysaccharides Osmotic Pressure Protein Binding Teichoic Acids |
| description |
The probiotic Gram-positive bacterium Lactobacillus casei BL23 is naturally confronted with salt-stress habitats. It has been previously reported that growth in high-salt medium, containing 0.8 M NaCl, leads to modifications in the cell envelope of this bacterium. In this study, we report that L. casei BL23 has an increased ability to form biofilms and to bind cations in high-salt conditions. This behaviour correlated with modifications of surface properties involving teichoic acids, which are important cell wall components. We also showed that, in these high-salt conditions, L. casei BL23 produces less of the cell wall polymer lipoteichoic acid (LTA), and that this anionic polymer has a shorter mean chain length and a lower level of D-alanyl-substitution. Analysis of the transcript levels of the dltABCD operon, encoding the enzymes required for the incorporation of D-alanine into anionic polymers, showed a 16-fold reduction in mRNA levels, which is consistent with a decrease in D-alanine substitutions on LTA. Furthermore, a 13-fold reduction in the transcript levels was observed for the gene LCABL_09330 coding for a putative LTA synthase. To provide further experimental evidence that LCABL_09330 is a true LTA synthase (LtaS) in L. casei BL23, the enzymic domain was cloned and expressed in E. coli. The purified protein was able to hydrolyse the membrane lipid phosphatidylglycerol as expected for an LTA synthase enzyme, and hence LCABL_09330 was renamed LtaS. The purified enzyme showed Mn2+-ion dependent activity, and its activity was modulated by differences in NaCl concentration. The decrease in both ltaS transcript levels and enzyme activity observed in high-salt conditions might influence the length of the LTA backbone chain. A putative function of the modified LTA structure is discussed that is compatible with the growth under salt-stress conditions and with the overall envelope modifications taking place during this stress condition. © 2013 SGM. |
| format |
JOUR |
| author |
Palomino, M.M. Allievi, M.C. Gründling, A. Sanchez-Rivas, C. Ruzal, S.M. |
| author_facet |
Palomino, M.M. Allievi, M.C. Gründling, A. Sanchez-Rivas, C. Ruzal, S.M. |
| author_sort |
Palomino, M.M. |
| title |
Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid |
| title_short |
Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid |
| title_full |
Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid |
| title_fullStr |
Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid |
| title_full_unstemmed |
Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid |
| title_sort |
osmotic stress adaptation in lactobacillus casei bl23 leads to structural changes in the cell wall polymer lipoteichoic acid |
| url |
http://hdl.handle.net/20.500.12110/paper_13500872_v159_nPART11_p2416_Palomino |
| work_keys_str_mv |
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| _version_ |
1807315886964473856 |