Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis

The role of Anr in oxidative stress resistance was investigated in Pseudomonas extremaustralis, a polyhydroxybutyrate-producing Antarctic bacterium. The absence of Anr caused increased sensitivity to hydrogen peroxide under low oxygen tension. This phenomenon was associated with a decrease in the re...

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Autores principales:	Tribelli, P.M., Nikel, P.I., Oppezzo, O.J., López, N.I.
Formato:	JOUR
Materias:	bacterial protein catalase exopolysaccharide hydrogen peroxide protein anr reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate thiol unclassified drug article bacterial genome bacterial strain binding site controlled study enzyme activity gene sequence Gram negative aerobic rods and cocci nonhuman oxidative stress oxygen consumption oxygen tension priority journal Pseudomonas extremaustralis redox stress wild type Bacterial Proteins DNA, Bacterial Gene Deletion Gene Expression Regulation, Bacterial Genome, Bacterial Hydrogen Peroxide Molecular Sequence Data Oxidation-Reduction Oxidative Stress Pseudomonas Sequence Analysis, DNA Stress, Physiological Sulfhydryl Compounds Transcription Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13500872_v159_n2_p259_Tribelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_13500872_v159_n2_p259_Tribelli2023-10-03T16:09:54Z Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis Tribelli, P.M. Nikel, P.I. Oppezzo, O.J. López, N.I. bacterial protein catalase exopolysaccharide hydrogen peroxide protein anr reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate thiol unclassified drug article bacterial genome bacterial strain binding site controlled study enzyme activity gene sequence Gram negative aerobic rods and cocci nonhuman oxidative stress oxygen consumption oxygen tension priority journal Pseudomonas extremaustralis redox stress wild type Bacterial Proteins DNA, Bacterial Gene Deletion Gene Expression Regulation, Bacterial Genome, Bacterial Hydrogen Peroxide Molecular Sequence Data Oxidation-Reduction Oxidative Stress Pseudomonas Sequence Analysis, DNA Stress, Physiological Sulfhydryl Compounds Transcription Factors The role of Anr in oxidative stress resistance was investigated in Pseudomonas extremaustralis, a polyhydroxybutyrate-producing Antarctic bacterium. The absence of Anr caused increased sensitivity to hydrogen peroxide under low oxygen tension. This phenomenon was associated with a decrease in the redox ratio, higher oxygen consumption and higher reactive oxygen species production. Physiological responses of the mutant to the oxidized state included an increase in NADP(H)content, catalase activity and exopolysaccharide production. The wild-type strain showed a sharp decrease in the reduced thiol pool when exposed to hydrogen peroxide, not observed in the mutant strain. In silico analysis of the genome sequence of P. extremaustralis revealed putative Anr binding sites upstream from genes related to oxidative stress. Genes encoding several chaperones and cold shock proteins, a glutathione synthase, a sulfate transporter and a thiol peroxidase were identified as potential targets for Anr regulation. Our results suggest a novel role for Anr in oxidative stress resistance and in redox balance maintenance under conditions of restricted oxygen supply. © 2013 SGM. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13500872_v159_n2_p259_Tribelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial protein catalase exopolysaccharide hydrogen peroxide protein anr reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate thiol unclassified drug article bacterial genome bacterial strain binding site controlled study enzyme activity gene sequence Gram negative aerobic rods and cocci nonhuman oxidative stress oxygen consumption oxygen tension priority journal Pseudomonas extremaustralis redox stress wild type Bacterial Proteins DNA, Bacterial Gene Deletion Gene Expression Regulation, Bacterial Genome, Bacterial Hydrogen Peroxide Molecular Sequence Data Oxidation-Reduction Oxidative Stress Pseudomonas Sequence Analysis, DNA Stress, Physiological Sulfhydryl Compounds Transcription Factors
spellingShingle	bacterial protein catalase exopolysaccharide hydrogen peroxide protein anr reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate thiol unclassified drug article bacterial genome bacterial strain binding site controlled study enzyme activity gene sequence Gram negative aerobic rods and cocci nonhuman oxidative stress oxygen consumption oxygen tension priority journal Pseudomonas extremaustralis redox stress wild type Bacterial Proteins DNA, Bacterial Gene Deletion Gene Expression Regulation, Bacterial Genome, Bacterial Hydrogen Peroxide Molecular Sequence Data Oxidation-Reduction Oxidative Stress Pseudomonas Sequence Analysis, DNA Stress, Physiological Sulfhydryl Compounds Transcription Factors Tribelli, P.M. Nikel, P.I. Oppezzo, O.J. López, N.I. Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis
topic_facet	bacterial protein catalase exopolysaccharide hydrogen peroxide protein anr reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate thiol unclassified drug article bacterial genome bacterial strain binding site controlled study enzyme activity gene sequence Gram negative aerobic rods and cocci nonhuman oxidative stress oxygen consumption oxygen tension priority journal Pseudomonas extremaustralis redox stress wild type Bacterial Proteins DNA, Bacterial Gene Deletion Gene Expression Regulation, Bacterial Genome, Bacterial Hydrogen Peroxide Molecular Sequence Data Oxidation-Reduction Oxidative Stress Pseudomonas Sequence Analysis, DNA Stress, Physiological Sulfhydryl Compounds Transcription Factors
description	The role of Anr in oxidative stress resistance was investigated in Pseudomonas extremaustralis, a polyhydroxybutyrate-producing Antarctic bacterium. The absence of Anr caused increased sensitivity to hydrogen peroxide under low oxygen tension. This phenomenon was associated with a decrease in the redox ratio, higher oxygen consumption and higher reactive oxygen species production. Physiological responses of the mutant to the oxidized state included an increase in NADP(H)content, catalase activity and exopolysaccharide production. The wild-type strain showed a sharp decrease in the reduced thiol pool when exposed to hydrogen peroxide, not observed in the mutant strain. In silico analysis of the genome sequence of P. extremaustralis revealed putative Anr binding sites upstream from genes related to oxidative stress. Genes encoding several chaperones and cold shock proteins, a glutathione synthase, a sulfate transporter and a thiol peroxidase were identified as potential targets for Anr regulation. Our results suggest a novel role for Anr in oxidative stress resistance and in redox balance maintenance under conditions of restricted oxygen supply. © 2013 SGM.
format	JOUR
author	Tribelli, P.M. Nikel, P.I. Oppezzo, O.J. López, N.I.
author_facet	Tribelli, P.M. Nikel, P.I. Oppezzo, O.J. López, N.I.
author_sort	Tribelli, P.M.
title	Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis
title_short	Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis
title_full	Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis
title_fullStr	Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis
title_full_unstemmed	Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis
title_sort	anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis
url	http://hdl.handle.net/20.500.12110/paper_13500872_v159_n2_p259_Tribelli
work_keys_str_mv	AT tribellipm anrtheanaerobicglobalregulatormodulatestheredoxstateandoxidativestressresistanceinpseudomonasextremaustralis AT nikelpi anrtheanaerobicglobalregulatormodulatestheredoxstateandoxidativestressresistanceinpseudomonasextremaustralis AT oppezzooj anrtheanaerobicglobalregulatormodulatestheredoxstateandoxidativestressresistanceinpseudomonasextremaustralis AT lopezni anrtheanaerobicglobalregulatormodulatestheredoxstateandoxidativestressresistanceinpseudomonasextremaustralis
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Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in pseudomonas extremaustralis

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