Isolation, culture and characterization of a new strain of Euglena gracilis
A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for...
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todo:paper_13220829_v52_n2_p168_Ruiz2023-10-03T16:09:25Z Isolation, culture and characterization of a new strain of Euglena gracilis Ruiz, L.B. Rocchetta, I. Dos Santos Ferreira, V. Conforti, V. Bleached Culture Euglena gracilis Lipid Protein Strain Sugar flagellate river pollution Euglena Euglena gracilis Euglena gracilis Mastigophora (flagellates) A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for MAT were compared with data obtained for a UTEX Culture Collection strain. Likewise, cells from both strains were bleached using streptomycin, and grown in the same media used for green samples. Both MAT and UTEX showed clear differences in their biochemical composition and growth rate depending on the media used. They also exhibited different growth patterns. E. gracilis medium proved to be the best culture environment for both strains either in autotrophic or heterotrophic conditions. Results show that basal contents of lipids, sugars, proteins and chlorophyll a vary depending on the strain, and thus values obtained for one strain do not apply to another. Moreover, strain origin may have an influence on the mechanisms of adaptation or defense developed by each sample. Fil:Ruiz, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Conforti, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13220829_v52_n2_p168_Ruiz |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Bleached Culture Euglena gracilis Lipid Protein Strain Sugar flagellate river pollution Euglena Euglena gracilis Euglena gracilis Mastigophora (flagellates) |
spellingShingle |
Bleached Culture Euglena gracilis Lipid Protein Strain Sugar flagellate river pollution Euglena Euglena gracilis Euglena gracilis Mastigophora (flagellates) Ruiz, L.B. Rocchetta, I. Dos Santos Ferreira, V. Conforti, V. Isolation, culture and characterization of a new strain of Euglena gracilis |
topic_facet |
Bleached Culture Euglena gracilis Lipid Protein Strain Sugar flagellate river pollution Euglena Euglena gracilis Euglena gracilis Mastigophora (flagellates) |
description |
A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for MAT were compared with data obtained for a UTEX Culture Collection strain. Likewise, cells from both strains were bleached using streptomycin, and grown in the same media used for green samples. Both MAT and UTEX showed clear differences in their biochemical composition and growth rate depending on the media used. They also exhibited different growth patterns. E. gracilis medium proved to be the best culture environment for both strains either in autotrophic or heterotrophic conditions. Results show that basal contents of lipids, sugars, proteins and chlorophyll a vary depending on the strain, and thus values obtained for one strain do not apply to another. Moreover, strain origin may have an influence on the mechanisms of adaptation or defense developed by each sample. |
format |
JOUR |
author |
Ruiz, L.B. Rocchetta, I. Dos Santos Ferreira, V. Conforti, V. |
author_facet |
Ruiz, L.B. Rocchetta, I. Dos Santos Ferreira, V. Conforti, V. |
author_sort |
Ruiz, L.B. |
title |
Isolation, culture and characterization of a new strain of Euglena gracilis |
title_short |
Isolation, culture and characterization of a new strain of Euglena gracilis |
title_full |
Isolation, culture and characterization of a new strain of Euglena gracilis |
title_fullStr |
Isolation, culture and characterization of a new strain of Euglena gracilis |
title_full_unstemmed |
Isolation, culture and characterization of a new strain of Euglena gracilis |
title_sort |
isolation, culture and characterization of a new strain of euglena gracilis |
url |
http://hdl.handle.net/20.500.12110/paper_13220829_v52_n2_p168_Ruiz |
work_keys_str_mv |
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1807319875839852544 |