Flammulina velutipes: An option for “alperujo” use
Background Two-phase olive-mill wastes (or “alperujo”) exhibit highly phytotoxic properties, mainly due to phenols. A valuable option for alperujo is its agricultural use, provided that no phytotoxic effects occur. Aims The present investigation was aimed at evaluating the efficacy of two strains of...
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todo:paper_11301406_v33_n4_p242_Rugolo2023-10-03T16:07:53Z Flammulina velutipes: An option for “alperujo” use Rugolo, M. Levin, L. Lechner, B.E. Alperujo Flammulina velutipes Lignocellulolytic enzymes Mushroom production cellulase endo beta 1,4 xylanase fungal enzyme laccase lignocellulolytic enzyme manganese peroxidase phenol unclassified drug phenol derivative agricultural waste Article concentration (parameters) detoxification edible mushroom enzyme activity enzyme substrate enzyme synthesis Flammulina velutipes fungal colonization fungal strain fungal structures fungus culture germination nonhuman phytotoxicity radish temperature waste management Agaricales agriculture Flammulina olive tree physiology procedures Agaricales Agriculture Flammulina Olea Phenols Background Two-phase olive-mill wastes (or “alperujo”) exhibit highly phytotoxic properties, mainly due to phenols. A valuable option for alperujo is its agricultural use, provided that no phytotoxic effects occur. Aims The present investigation was aimed at evaluating the efficacy of two strains of the lignin-degrading fungus Flammulina velutipes to colonize alperujo in order to produce edible mushrooms and to achieve its detoxification. Methods Some important cultural characters related to mushroom production (earliness, biological efficiency and quality of basidiomes) were estimated. The production of lignocellulolytic enzymes, phenol removal and detoxification of the substrate was evaluated. Results High biological efficiencies (70.8%) were obtained at 12 °C with F. velutipes strain BAFC 670/06 in a substrate containing poplar wood shavings and 90% of alperujo. The nature of the substrate did not seem to exert an important influence on pileus and stem morphology; nevertheless shortest stems were observed at higher temperatures. Endo-β-1,4-glucanase, endo-β-1,4-xylanase, laccase and Mn-peroxidase activities were detected in the extracts recovered from the solid-state cultures. Both F. velutipes strains were effective in removing the phenolic compounds. The initial concentration in the substrate with 90% alperujo was reduced in the case of F. velutipes BAFC 1763 by 84.31%, and 40.15% by F. velutipes BAFC 670/06. Germinability experiments on Raphanus sativus, showed that alperujo phytotoxicity was significantly reduced by F. velutipes cultures. Conclusions The experimented changes by the spent mushroom substrate resulting from F. velutipes cultivation with high amount of alperujo would allow its reuse for agricultural purposes. © 2016 Asociación Española de Micología Fil:Rugolo, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lechner, B.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_11301406_v33_n4_p242_Rugolo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alperujo Flammulina velutipes Lignocellulolytic enzymes Mushroom production cellulase endo beta 1,4 xylanase fungal enzyme laccase lignocellulolytic enzyme manganese peroxidase phenol unclassified drug phenol derivative agricultural waste Article concentration (parameters) detoxification edible mushroom enzyme activity enzyme substrate enzyme synthesis Flammulina velutipes fungal colonization fungal strain fungal structures fungus culture germination nonhuman phytotoxicity radish temperature waste management Agaricales agriculture Flammulina olive tree physiology procedures Agaricales Agriculture Flammulina Olea Phenols |
| spellingShingle |
Alperujo Flammulina velutipes Lignocellulolytic enzymes Mushroom production cellulase endo beta 1,4 xylanase fungal enzyme laccase lignocellulolytic enzyme manganese peroxidase phenol unclassified drug phenol derivative agricultural waste Article concentration (parameters) detoxification edible mushroom enzyme activity enzyme substrate enzyme synthesis Flammulina velutipes fungal colonization fungal strain fungal structures fungus culture germination nonhuman phytotoxicity radish temperature waste management Agaricales agriculture Flammulina olive tree physiology procedures Agaricales Agriculture Flammulina Olea Phenols Rugolo, M. Levin, L. Lechner, B.E. Flammulina velutipes: An option for “alperujo” use |
| topic_facet |
Alperujo Flammulina velutipes Lignocellulolytic enzymes Mushroom production cellulase endo beta 1,4 xylanase fungal enzyme laccase lignocellulolytic enzyme manganese peroxidase phenol unclassified drug phenol derivative agricultural waste Article concentration (parameters) detoxification edible mushroom enzyme activity enzyme substrate enzyme synthesis Flammulina velutipes fungal colonization fungal strain fungal structures fungus culture germination nonhuman phytotoxicity radish temperature waste management Agaricales agriculture Flammulina olive tree physiology procedures Agaricales Agriculture Flammulina Olea Phenols |
| description |
Background Two-phase olive-mill wastes (or “alperujo”) exhibit highly phytotoxic properties, mainly due to phenols. A valuable option for alperujo is its agricultural use, provided that no phytotoxic effects occur. Aims The present investigation was aimed at evaluating the efficacy of two strains of the lignin-degrading fungus Flammulina velutipes to colonize alperujo in order to produce edible mushrooms and to achieve its detoxification. Methods Some important cultural characters related to mushroom production (earliness, biological efficiency and quality of basidiomes) were estimated. The production of lignocellulolytic enzymes, phenol removal and detoxification of the substrate was evaluated. Results High biological efficiencies (70.8%) were obtained at 12 °C with F. velutipes strain BAFC 670/06 in a substrate containing poplar wood shavings and 90% of alperujo. The nature of the substrate did not seem to exert an important influence on pileus and stem morphology; nevertheless shortest stems were observed at higher temperatures. Endo-β-1,4-glucanase, endo-β-1,4-xylanase, laccase and Mn-peroxidase activities were detected in the extracts recovered from the solid-state cultures. Both F. velutipes strains were effective in removing the phenolic compounds. The initial concentration in the substrate with 90% alperujo was reduced in the case of F. velutipes BAFC 1763 by 84.31%, and 40.15% by F. velutipes BAFC 670/06. Germinability experiments on Raphanus sativus, showed that alperujo phytotoxicity was significantly reduced by F. velutipes cultures. Conclusions The experimented changes by the spent mushroom substrate resulting from F. velutipes cultivation with high amount of alperujo would allow its reuse for agricultural purposes. © 2016 Asociación Española de Micología |
| format |
JOUR |
| author |
Rugolo, M. Levin, L. Lechner, B.E. |
| author_facet |
Rugolo, M. Levin, L. Lechner, B.E. |
| author_sort |
Rugolo, M. |
| title |
Flammulina velutipes: An option for “alperujo” use |
| title_short |
Flammulina velutipes: An option for “alperujo” use |
| title_full |
Flammulina velutipes: An option for “alperujo” use |
| title_fullStr |
Flammulina velutipes: An option for “alperujo” use |
| title_full_unstemmed |
Flammulina velutipes: An option for “alperujo” use |
| title_sort |
flammulina velutipes: an option for “alperujo” use |
| url |
http://hdl.handle.net/20.500.12110/paper_11301406_v33_n4_p242_Rugolo |
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AT rugolom flammulinavelutipesanoptionforalperujouse AT levinl flammulinavelutipesanoptionforalperujouse AT lechnerbe flammulinavelutipesanoptionforalperujouse |
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