Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10988823_v107_n_p85_Rostoker |
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todo:paper_10988823_v107_n_p85_Rostoker2023-10-03T16:06:32Z Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype Rostoker, R. Yaseen, H. Schif-Zuck, S. Lichtenstein, R.G. Rabinovich, G.A. Ariel, A. 15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation arachidonate 12 lipoxygenase arachidonate 15 lipoxygenase CD11b antigen galectin 1 interleukin 10 interleukin 1beta lipopolysaccharide tumor necrosis factor alpha animal cell animal experiment animal model article controlled study cytokine release down regulation enzyme activity ex vivo study immunoregulation in vivo study macrophage male mouse nonhuman peritoneum macrophage phagocytosis phenotype protein expression upregulation 15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation Animals Antigens, CD11 Apoptosis Arachidonate 12-Lipoxygenase Arachidonate 15-Lipoxygenase Cells, Cultured Cytokines Enzyme Induction Galectin 1 Inflammation Lipopolysaccharides Macrophages, Peritoneal Male Mice Mice, Inbred C57BL Phenotype During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11b low macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation. © 2013 Elsevier Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10988823_v107_n_p85_Rostoker |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation arachidonate 12 lipoxygenase arachidonate 15 lipoxygenase CD11b antigen galectin 1 interleukin 10 interleukin 1beta lipopolysaccharide tumor necrosis factor alpha animal cell animal experiment animal model article controlled study cytokine release down regulation enzyme activity ex vivo study immunoregulation in vivo study macrophage male mouse nonhuman peritoneum macrophage phagocytosis phenotype protein expression upregulation 15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation Animals Antigens, CD11 Apoptosis Arachidonate 12-Lipoxygenase Arachidonate 15-Lipoxygenase Cells, Cultured Cytokines Enzyme Induction Galectin 1 Inflammation Lipopolysaccharides Macrophages, Peritoneal Male Mice Mice, Inbred C57BL Phenotype |
| spellingShingle |
15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation arachidonate 12 lipoxygenase arachidonate 15 lipoxygenase CD11b antigen galectin 1 interleukin 10 interleukin 1beta lipopolysaccharide tumor necrosis factor alpha animal cell animal experiment animal model article controlled study cytokine release down regulation enzyme activity ex vivo study immunoregulation in vivo study macrophage male mouse nonhuman peritoneum macrophage phagocytosis phenotype protein expression upregulation 15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation Animals Antigens, CD11 Apoptosis Arachidonate 12-Lipoxygenase Arachidonate 15-Lipoxygenase Cells, Cultured Cytokines Enzyme Induction Galectin 1 Inflammation Lipopolysaccharides Macrophages, Peritoneal Male Mice Mice, Inbred C57BL Phenotype Rostoker, R. Yaseen, H. Schif-Zuck, S. Lichtenstein, R.G. Rabinovich, G.A. Ariel, A. Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype |
| topic_facet |
15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation arachidonate 12 lipoxygenase arachidonate 15 lipoxygenase CD11b antigen galectin 1 interleukin 10 interleukin 1beta lipopolysaccharide tumor necrosis factor alpha animal cell animal experiment animal model article controlled study cytokine release down regulation enzyme activity ex vivo study immunoregulation in vivo study macrophage male mouse nonhuman peritoneum macrophage phagocytosis phenotype protein expression upregulation 15-Lipoxygenase Efferocytosis Galectin-1 Macrophages Resolution of inflammation Animals Antigens, CD11 Apoptosis Arachidonate 12-Lipoxygenase Arachidonate 15-Lipoxygenase Cells, Cultured Cytokines Enzyme Induction Galectin 1 Inflammation Lipopolysaccharides Macrophages, Peritoneal Male Mice Mice, Inbred C57BL Phenotype |
| description |
During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11b low macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation. © 2013 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Rostoker, R. Yaseen, H. Schif-Zuck, S. Lichtenstein, R.G. Rabinovich, G.A. Ariel, A. |
| author_facet |
Rostoker, R. Yaseen, H. Schif-Zuck, S. Lichtenstein, R.G. Rabinovich, G.A. Ariel, A. |
| author_sort |
Rostoker, R. |
| title |
Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype |
| title_short |
Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype |
| title_full |
Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype |
| title_fullStr |
Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype |
| title_full_unstemmed |
Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype |
| title_sort |
galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype |
| url |
http://hdl.handle.net/20.500.12110/paper_10988823_v107_n_p85_Rostoker |
| work_keys_str_mv |
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1807320733072752640 |