Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can al...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10972765_v4_n2_p251_Cramer |
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todo:paper_10972765_v4_n2_p251_Cramer2023-10-03T16:05:28Z Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer Cramer, P. Cáceres, J.F. Cazalla, D. Kadener, S. Muro, A.F. Baralle, F.E. Kornblihtt, A.R. alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10972765_v4_n2_p251_Cramer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation |
| spellingShingle |
alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation Cramer, P. Cáceres, J.F. Cazalla, D. Kadener, S. Muro, A.F. Baralle, F.E. Kornblihtt, A.R. Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer |
| topic_facet |
alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation |
| description |
Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE. |
| format |
JOUR |
| author |
Cramer, P. Cáceres, J.F. Cazalla, D. Kadener, S. Muro, A.F. Baralle, F.E. Kornblihtt, A.R. |
| author_facet |
Cramer, P. Cáceres, J.F. Cazalla, D. Kadener, S. Muro, A.F. Baralle, F.E. Kornblihtt, A.R. |
| author_sort |
Cramer, P. |
| title |
Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer |
| title_short |
Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer |
| title_full |
Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer |
| title_fullStr |
Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer |
| title_full_unstemmed |
Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer |
| title_sort |
coupling of transcription with alternative splicing: rna pol ii promoters modulate sf2/asf and 9g8 effects on an exonic splicing enhancer |
| url |
http://hdl.handle.net/20.500.12110/paper_10972765_v4_n2_p251_Cramer |
| work_keys_str_mv |
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