Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress
Glyphosate-based herbicides (GF) are extensively used for weed control. Thus, it is important to investigate their putative toxic effects. We have reported that GF at subagriculture concentrations inhibits proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. In this investigation,...
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todo:paper_10956670_v30_n8_p404_Martini2023-10-03T16:05:22Z Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress Martini, C.N. Gabrielli, M. Brandani, J.N. Vila, M.D.C. 3T3-L1 Fibroblasts Differentiation Glyphosate Based-Herbicide MEFs Oxidative Stress CCAAT enhancer binding protein beta fat droplet glyphosate hydrogen peroxide messenger RNA peroxisome proliferator activated receptor gamma superoxide dismutase 3T3 cell line adipogenesis animal cell Article cell differentiation cell proliferation controlled study dilution embryo enzyme activity immunofluorescence lipid peroxidation lipid storage mouse nonhuman oxidative stress proadipocyte reverse transcription polymerase chain reaction upregulation Western blotting Glyphosate-based herbicides (GF) are extensively used for weed control. Thus, it is important to investigate their putative toxic effects. We have reported that GF at subagriculture concentrations inhibits proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. In this investigation, we evaluated the effect of GF on genes upregulated during adipogenesis. GF was able to inhibit the induction of PPAR gamma, the master gene in adipogenesis but not C/EBP beta, which precedes PPAR gamma activation. GF also inhibited differentiation and proliferation of another model of preadipocyte: mouse embryonic fibroblasts. In exponentially growing 3T3-L1 cells, GF increased lipid peroxidation and the activity of the antioxidant enzyme, superoxide dismutase. We also found that proliferation was inhibited with lower concentrations of GF when time of exposure was extended. Thus, GF was able to inhibit proliferation and differentiation of preadipocytes and to induce oxidative stress, which is indicative of its ability to alter cellular physiology. © 2016 Wiley Periodicals, Inc. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabrielli, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10956670_v30_n8_p404_Martini |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3T3-L1 Fibroblasts Differentiation Glyphosate Based-Herbicide MEFs Oxidative Stress CCAAT enhancer binding protein beta fat droplet glyphosate hydrogen peroxide messenger RNA peroxisome proliferator activated receptor gamma superoxide dismutase 3T3 cell line adipogenesis animal cell Article cell differentiation cell proliferation controlled study dilution embryo enzyme activity immunofluorescence lipid peroxidation lipid storage mouse nonhuman oxidative stress proadipocyte reverse transcription polymerase chain reaction upregulation Western blotting |
| spellingShingle |
3T3-L1 Fibroblasts Differentiation Glyphosate Based-Herbicide MEFs Oxidative Stress CCAAT enhancer binding protein beta fat droplet glyphosate hydrogen peroxide messenger RNA peroxisome proliferator activated receptor gamma superoxide dismutase 3T3 cell line adipogenesis animal cell Article cell differentiation cell proliferation controlled study dilution embryo enzyme activity immunofluorescence lipid peroxidation lipid storage mouse nonhuman oxidative stress proadipocyte reverse transcription polymerase chain reaction upregulation Western blotting Martini, C.N. Gabrielli, M. Brandani, J.N. Vila, M.D.C. Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress |
| topic_facet |
3T3-L1 Fibroblasts Differentiation Glyphosate Based-Herbicide MEFs Oxidative Stress CCAAT enhancer binding protein beta fat droplet glyphosate hydrogen peroxide messenger RNA peroxisome proliferator activated receptor gamma superoxide dismutase 3T3 cell line adipogenesis animal cell Article cell differentiation cell proliferation controlled study dilution embryo enzyme activity immunofluorescence lipid peroxidation lipid storage mouse nonhuman oxidative stress proadipocyte reverse transcription polymerase chain reaction upregulation Western blotting |
| description |
Glyphosate-based herbicides (GF) are extensively used for weed control. Thus, it is important to investigate their putative toxic effects. We have reported that GF at subagriculture concentrations inhibits proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. In this investigation, we evaluated the effect of GF on genes upregulated during adipogenesis. GF was able to inhibit the induction of PPAR gamma, the master gene in adipogenesis but not C/EBP beta, which precedes PPAR gamma activation. GF also inhibited differentiation and proliferation of another model of preadipocyte: mouse embryonic fibroblasts. In exponentially growing 3T3-L1 cells, GF increased lipid peroxidation and the activity of the antioxidant enzyme, superoxide dismutase. We also found that proliferation was inhibited with lower concentrations of GF when time of exposure was extended. Thus, GF was able to inhibit proliferation and differentiation of preadipocytes and to induce oxidative stress, which is indicative of its ability to alter cellular physiology. © 2016 Wiley Periodicals, Inc. |
| format |
JOUR |
| author |
Martini, C.N. Gabrielli, M. Brandani, J.N. Vila, M.D.C. |
| author_facet |
Martini, C.N. Gabrielli, M. Brandani, J.N. Vila, M.D.C. |
| author_sort |
Martini, C.N. |
| title |
Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress |
| title_short |
Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress |
| title_full |
Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress |
| title_fullStr |
Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress |
| title_full_unstemmed |
Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress |
| title_sort |
glyphosate inhibits ppar gamma induction and differentiation of preadipocytes and is able to induce oxidative stress |
| url |
http://hdl.handle.net/20.500.12110/paper_10956670_v30_n8_p404_Martini |
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