Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP
Cyclic AMP (cAMP) and dopamine modulate ion uptake across isolated and perfused posterior gills of Chasmagnathus granulatus acclimated to 10‰ salinity. Addition of cAMP agonists, such as cp-cAMP, forskolin, and IBMX, produced a significant increase in the transepithelial potential difference (Vte),...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10956433_v139_n1_p103_Halperin |
| Aporte de: |
| id |
todo:paper_10956433_v139_n1_p103_Halperin |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_10956433_v139_n1_p103_Halperin2023-10-03T16:05:20Z Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP Halperin, J. Genovese, G. Tresguerres, M. Luquet, C.M. cAMP Chasmagnathus granulatus Crab Dopamine Gills Ion transport PKA Transepithelial potential differences adenosine triphosphatase cyclic AMP cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor dopamine forskolin ion isobutylmethylxanthine kt 5720 potassium ion sodium ion article Chasmagnathus granulatus concentration (parameters) correlation analysis crab dose response enzyme activity gill ion transport male nonhuman potential difference salinity sodium transport Chasmagnathus granulata Decapoda (Crustacea) Cyclic AMP (cAMP) and dopamine modulate ion uptake across isolated and perfused posterior gills of Chasmagnathus granulatus acclimated to 10‰ salinity. Addition of cAMP agonists, such as cp-cAMP, forskolin, and IBMX, produced a significant increase in the transepithelial potential difference (Vte), which reflects ion transport activity. Dopamine (DA) also had a stimulatory effect on ion uptake, increasing Vte and Na+ influx, although this effect was transient, since both variables remained elevated for less than 30 min. In addition, the dose-response curve for DA concentration-Vte was biphasic, and the maximum stimulation was obtained with 10 μmol l -1. When the effects of forskolin and DA on the Na+/K +-ATPase activity were tested, they correlated well with the Vte and Na+ influx experiments; the enzyme activity increased significantly after preincubation of gill fragments for 10 min with forskolin or DA (51 and 64%, respectively), but there was no effect after pre-incubation with DA for 20 min. Finally, KT5720, a specific inhibitor of cAMP-dependent protein kinase (PKA), completely abolished the stimulatory effect of DA on Vte, suggesting the involvement of PKA in this mechanism. © 2004 Elsevier Inc. All rights reserved. Fil:Halperin, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tresguerres, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10956433_v139_n1_p103_Halperin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cAMP Chasmagnathus granulatus Crab Dopamine Gills Ion transport PKA Transepithelial potential differences adenosine triphosphatase cyclic AMP cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor dopamine forskolin ion isobutylmethylxanthine kt 5720 potassium ion sodium ion article Chasmagnathus granulatus concentration (parameters) correlation analysis crab dose response enzyme activity gill ion transport male nonhuman potential difference salinity sodium transport Chasmagnathus granulata Decapoda (Crustacea) |
| spellingShingle |
cAMP Chasmagnathus granulatus Crab Dopamine Gills Ion transport PKA Transepithelial potential differences adenosine triphosphatase cyclic AMP cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor dopamine forskolin ion isobutylmethylxanthine kt 5720 potassium ion sodium ion article Chasmagnathus granulatus concentration (parameters) correlation analysis crab dose response enzyme activity gill ion transport male nonhuman potential difference salinity sodium transport Chasmagnathus granulata Decapoda (Crustacea) Halperin, J. Genovese, G. Tresguerres, M. Luquet, C.M. Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP |
| topic_facet |
cAMP Chasmagnathus granulatus Crab Dopamine Gills Ion transport PKA Transepithelial potential differences adenosine triphosphatase cyclic AMP cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor dopamine forskolin ion isobutylmethylxanthine kt 5720 potassium ion sodium ion article Chasmagnathus granulatus concentration (parameters) correlation analysis crab dose response enzyme activity gill ion transport male nonhuman potential difference salinity sodium transport Chasmagnathus granulata Decapoda (Crustacea) |
| description |
Cyclic AMP (cAMP) and dopamine modulate ion uptake across isolated and perfused posterior gills of Chasmagnathus granulatus acclimated to 10‰ salinity. Addition of cAMP agonists, such as cp-cAMP, forskolin, and IBMX, produced a significant increase in the transepithelial potential difference (Vte), which reflects ion transport activity. Dopamine (DA) also had a stimulatory effect on ion uptake, increasing Vte and Na+ influx, although this effect was transient, since both variables remained elevated for less than 30 min. In addition, the dose-response curve for DA concentration-Vte was biphasic, and the maximum stimulation was obtained with 10 μmol l -1. When the effects of forskolin and DA on the Na+/K +-ATPase activity were tested, they correlated well with the Vte and Na+ influx experiments; the enzyme activity increased significantly after preincubation of gill fragments for 10 min with forskolin or DA (51 and 64%, respectively), but there was no effect after pre-incubation with DA for 20 min. Finally, KT5720, a specific inhibitor of cAMP-dependent protein kinase (PKA), completely abolished the stimulatory effect of DA on Vte, suggesting the involvement of PKA in this mechanism. © 2004 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Halperin, J. Genovese, G. Tresguerres, M. Luquet, C.M. |
| author_facet |
Halperin, J. Genovese, G. Tresguerres, M. Luquet, C.M. |
| author_sort |
Halperin, J. |
| title |
Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP |
| title_short |
Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP |
| title_full |
Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP |
| title_fullStr |
Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP |
| title_full_unstemmed |
Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP |
| title_sort |
modulation of ion uptake across posterior gills of the crab chasmagnathus granulatus by dopamine and camp |
| url |
http://hdl.handle.net/20.500.12110/paper_10956433_v139_n1_p103_Halperin |
| work_keys_str_mv |
AT halperinj modulationofionuptakeacrossposteriorgillsofthecrabchasmagnathusgranulatusbydopamineandcamp AT genoveseg modulationofionuptakeacrossposteriorgillsofthecrabchasmagnathusgranulatusbydopamineandcamp AT tresguerresm modulationofionuptakeacrossposteriorgillsofthecrabchasmagnathusgranulatusbydopamineandcamp AT luquetcm modulationofionuptakeacrossposteriorgillsofthecrabchasmagnathusgranulatusbydopamineandcamp |
| _version_ |
1782029796604116992 |