Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface
We demonstrate an easy-to-implement scheme for fluorescence enhancement and observation volume reduction using photonic crystals (PhCs) as substrates for microscopy. By normal incidence coupling to slow 2D-PhC guided modes, a 65 fold enhancement in the excitation is achieved in the near field region...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10944087_v18_n4_p3693_Estrada |
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todo:paper_10944087_v18_n4_p3693_Estrada2023-10-03T16:05:11Z Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface Estrada, L.C. Martinez, O.E. Brunstein, M. Bouchoule, S. Le-Gratiet, L. Talneau, A. Sagnes, I. Monnier, P. Levenson, J.A. Yacomotti, A.M. Fluorescence Fluorescence microscopy Substrates Two dimensional 2-D photonic crystals Fluorescence enhancement Guided modes High spatial resolution Near field region Normal incidence Resonant mode Volume reductions Photonic crystals contrast medium fluorescent dye article chemistry crystallization fluorescence microscopy image enhancement methodology surface property Contrast Media Crystallization Fluorescent Dyes Image Enhancement Microscopy, Fluorescence Surface Properties We demonstrate an easy-to-implement scheme for fluorescence enhancement and observation volume reduction using photonic crystals (PhCs) as substrates for microscopy. By normal incidence coupling to slow 2D-PhC guided modes, a 65 fold enhancement in the excitation is achieved in the near field region (100 nm deep and 1 μm wide) of the resonant mode. Such large enhancement together with the high spatial resolution makes this device an excellent substrate for fluorescence microscopies. © 2010 Optical Society of America. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10944087_v18_n4_p3693_Estrada |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Fluorescence Fluorescence microscopy Substrates Two dimensional 2-D photonic crystals Fluorescence enhancement Guided modes High spatial resolution Near field region Normal incidence Resonant mode Volume reductions Photonic crystals contrast medium fluorescent dye article chemistry crystallization fluorescence microscopy image enhancement methodology surface property Contrast Media Crystallization Fluorescent Dyes Image Enhancement Microscopy, Fluorescence Surface Properties |
spellingShingle |
Fluorescence Fluorescence microscopy Substrates Two dimensional 2-D photonic crystals Fluorescence enhancement Guided modes High spatial resolution Near field region Normal incidence Resonant mode Volume reductions Photonic crystals contrast medium fluorescent dye article chemistry crystallization fluorescence microscopy image enhancement methodology surface property Contrast Media Crystallization Fluorescent Dyes Image Enhancement Microscopy, Fluorescence Surface Properties Estrada, L.C. Martinez, O.E. Brunstein, M. Bouchoule, S. Le-Gratiet, L. Talneau, A. Sagnes, I. Monnier, P. Levenson, J.A. Yacomotti, A.M. Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface |
topic_facet |
Fluorescence Fluorescence microscopy Substrates Two dimensional 2-D photonic crystals Fluorescence enhancement Guided modes High spatial resolution Near field region Normal incidence Resonant mode Volume reductions Photonic crystals contrast medium fluorescent dye article chemistry crystallization fluorescence microscopy image enhancement methodology surface property Contrast Media Crystallization Fluorescent Dyes Image Enhancement Microscopy, Fluorescence Surface Properties |
description |
We demonstrate an easy-to-implement scheme for fluorescence enhancement and observation volume reduction using photonic crystals (PhCs) as substrates for microscopy. By normal incidence coupling to slow 2D-PhC guided modes, a 65 fold enhancement in the excitation is achieved in the near field region (100 nm deep and 1 μm wide) of the resonant mode. Such large enhancement together with the high spatial resolution makes this device an excellent substrate for fluorescence microscopies. © 2010 Optical Society of America. |
format |
JOUR |
author |
Estrada, L.C. Martinez, O.E. Brunstein, M. Bouchoule, S. Le-Gratiet, L. Talneau, A. Sagnes, I. Monnier, P. Levenson, J.A. Yacomotti, A.M. |
author_facet |
Estrada, L.C. Martinez, O.E. Brunstein, M. Bouchoule, S. Le-Gratiet, L. Talneau, A. Sagnes, I. Monnier, P. Levenson, J.A. Yacomotti, A.M. |
author_sort |
Estrada, L.C. |
title |
Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface |
title_short |
Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface |
title_full |
Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface |
title_fullStr |
Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface |
title_full_unstemmed |
Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface |
title_sort |
small volume excitation and enhancement of dye fluorescence on a 2d photonic crystal surface |
url |
http://hdl.handle.net/20.500.12110/paper_10944087_v18_n4_p3693_Estrada |
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