Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river
Field and laboratory experiments were combined to evaluate biomarker responses of Diplodon chilensis to sewage pollution. Mussels from an unpolluted area in Lacar lake (S0) were caged at a reference site (S1) and at two sites with increasing sewage pollution (S2, S3) in Pocahullo river (all in Argen...
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todo:paper_10934529_v49_n11_p1276_Bianchi2023-10-03T16:05:00Z Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river Bianchi, V.A. Rocchetta, I. Luquet, C.M. detoxifying defenses Fecal coliforms oxidative stress short and long-term response. Biomarkers Coliform bacteria Molluscs Oxidative stress Pollution Sewage detoxifying defenses Fecal coliforms Freshwater mussels Glutathione-S-transferase Laboratory experiments Lipid peroxidation short and long-term response Transplanted mussels River pollution biological marker catalase glutathione transferase thiobarbituric acid reactive substance biological marker catalase glutathione transferase sewage water pollutant adult animal tissue article controlled study Diplodon chilensis environmental exposure enzyme activity exocrine gland feces microflora gill lake lipid peroxidation mussel nonhuman oxidative stress river sewage sewage pollution water pollution analysis animal Argentina bivalve chemistry environmental monitoring metabolism sewage water pollutant water pollution Animals Argentina Biological Markers Bivalvia Catalase Environmental Monitoring Glutathione Transferase Lipid Peroxidation Rivers Sewage Water Pollutants, Chemical Water Pollution, Chemical Field and laboratory experiments were combined to evaluate biomarker responses of Diplodon chilensis to sewage pollution. Mussels from an unpolluted area in Lacar lake (S0) were caged at a reference site (S1) and at two sites with increasing sewage pollution (S2, S3) in Pocahullo river (all in Argentina). After 1 month, gill (g) glutathione S-transferase (GST) and catalase (CAT) activities, and lipid peroxidation (TBARS) were found to be significantly elevated in S3, gGST being positively correlated with fecal bacteria (FC) concentration. Digestive gland (dg) enzyme activities were depressed and dgTBARS were increased in all transplanted mussels. After 3 mo, most variables returned to control levels in S1 mussels except for dgCAT and dgTBARS. After seven months, GST and CAT activities of S0 and S3 mussels were evaluated in the laboratory, before and after acute exposure (8 h) to high fecal bacteria concentration ([FC] in S3x 2). gGST increased in both groups, while dgGST responded only in S3 mussels. gCAT and dgCAT activities were similarly increased by acute exposure in both groups. Our results suggest that gGST and gCAT are suitable biomarkers for high FC pollution regardless of previous exposure history. In addition, we show that dgCAT is sensitive to the acute increase in FC load, both in naive and long-term exposed individuals, while dgGST becomes responsive after long-term acclimatization. © 2014 Copyright © Taylor & Francis Group, LLC. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10934529_v49_n11_p1276_Bianchi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
detoxifying defenses Fecal coliforms oxidative stress short and long-term response. Biomarkers Coliform bacteria Molluscs Oxidative stress Pollution Sewage detoxifying defenses Fecal coliforms Freshwater mussels Glutathione-S-transferase Laboratory experiments Lipid peroxidation short and long-term response Transplanted mussels River pollution biological marker catalase glutathione transferase thiobarbituric acid reactive substance biological marker catalase glutathione transferase sewage water pollutant adult animal tissue article controlled study Diplodon chilensis environmental exposure enzyme activity exocrine gland feces microflora gill lake lipid peroxidation mussel nonhuman oxidative stress river sewage sewage pollution water pollution analysis animal Argentina bivalve chemistry environmental monitoring metabolism sewage water pollutant water pollution Animals Argentina Biological Markers Bivalvia Catalase Environmental Monitoring Glutathione Transferase Lipid Peroxidation Rivers Sewage Water Pollutants, Chemical Water Pollution, Chemical |
| spellingShingle |
detoxifying defenses Fecal coliforms oxidative stress short and long-term response. Biomarkers Coliform bacteria Molluscs Oxidative stress Pollution Sewage detoxifying defenses Fecal coliforms Freshwater mussels Glutathione-S-transferase Laboratory experiments Lipid peroxidation short and long-term response Transplanted mussels River pollution biological marker catalase glutathione transferase thiobarbituric acid reactive substance biological marker catalase glutathione transferase sewage water pollutant adult animal tissue article controlled study Diplodon chilensis environmental exposure enzyme activity exocrine gland feces microflora gill lake lipid peroxidation mussel nonhuman oxidative stress river sewage sewage pollution water pollution analysis animal Argentina bivalve chemistry environmental monitoring metabolism sewage water pollutant water pollution Animals Argentina Biological Markers Bivalvia Catalase Environmental Monitoring Glutathione Transferase Lipid Peroxidation Rivers Sewage Water Pollutants, Chemical Water Pollution, Chemical Bianchi, V.A. Rocchetta, I. Luquet, C.M. Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river |
| topic_facet |
detoxifying defenses Fecal coliforms oxidative stress short and long-term response. Biomarkers Coliform bacteria Molluscs Oxidative stress Pollution Sewage detoxifying defenses Fecal coliforms Freshwater mussels Glutathione-S-transferase Laboratory experiments Lipid peroxidation short and long-term response Transplanted mussels River pollution biological marker catalase glutathione transferase thiobarbituric acid reactive substance biological marker catalase glutathione transferase sewage water pollutant adult animal tissue article controlled study Diplodon chilensis environmental exposure enzyme activity exocrine gland feces microflora gill lake lipid peroxidation mussel nonhuman oxidative stress river sewage sewage pollution water pollution analysis animal Argentina bivalve chemistry environmental monitoring metabolism sewage water pollutant water pollution Animals Argentina Biological Markers Bivalvia Catalase Environmental Monitoring Glutathione Transferase Lipid Peroxidation Rivers Sewage Water Pollutants, Chemical Water Pollution, Chemical |
| description |
Field and laboratory experiments were combined to evaluate biomarker responses of Diplodon chilensis to sewage pollution. Mussels from an unpolluted area in Lacar lake (S0) were caged at a reference site (S1) and at two sites with increasing sewage pollution (S2, S3) in Pocahullo river (all in Argentina). After 1 month, gill (g) glutathione S-transferase (GST) and catalase (CAT) activities, and lipid peroxidation (TBARS) were found to be significantly elevated in S3, gGST being positively correlated with fecal bacteria (FC) concentration. Digestive gland (dg) enzyme activities were depressed and dgTBARS were increased in all transplanted mussels. After 3 mo, most variables returned to control levels in S1 mussels except for dgCAT and dgTBARS. After seven months, GST and CAT activities of S0 and S3 mussels were evaluated in the laboratory, before and after acute exposure (8 h) to high fecal bacteria concentration ([FC] in S3x 2). gGST increased in both groups, while dgGST responded only in S3 mussels. gCAT and dgCAT activities were similarly increased by acute exposure in both groups. Our results suggest that gGST and gCAT are suitable biomarkers for high FC pollution regardless of previous exposure history. In addition, we show that dgCAT is sensitive to the acute increase in FC load, both in naive and long-term exposed individuals, while dgGST becomes responsive after long-term acclimatization. © 2014 Copyright © Taylor & Francis Group, LLC. |
| format |
JOUR |
| author |
Bianchi, V.A. Rocchetta, I. Luquet, C.M. |
| author_facet |
Bianchi, V.A. Rocchetta, I. Luquet, C.M. |
| author_sort |
Bianchi, V.A. |
| title |
Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river |
| title_short |
Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river |
| title_full |
Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river |
| title_fullStr |
Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river |
| title_full_unstemmed |
Biomarker responses to sewage pollution in freshwater mussels (Diplodon chilensis) transplanted to a Patagonian river |
| title_sort |
biomarker responses to sewage pollution in freshwater mussels (diplodon chilensis) transplanted to a patagonian river |
| url |
http://hdl.handle.net/20.500.12110/paper_10934529_v49_n11_p1276_Bianchi |
| work_keys_str_mv |
AT bianchiva biomarkerresponsestosewagepollutioninfreshwatermusselsdiplodonchilensistransplantedtoapatagonianriver AT rocchettai biomarkerresponsestosewagepollutioninfreshwatermusselsdiplodonchilensistransplantedtoapatagonianriver AT luquetcm biomarkerresponsestosewagepollutioninfreshwatermusselsdiplodonchilensistransplantedtoapatagonianriver |
| _version_ |
1807318129486856192 |