Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulato...
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todo:paper_10898603_v37_n1_p1_Astort2023-10-03T16:04:55Z Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction Astort, F. Mercau, M. Giordanino, E. Degese, M.S. Caldareri, L. Coso, O. Cymeryng, C.B. cGMP Heme oxygenase 1 Nitric oxide Nuclear factor-erythroid 2-related factor (Nrf2) Soluble guanylate cyclase cyclic GMP dactinomycin diethylenetriamine guanylate cyclase guanylate cyclase inhibitor heme oxygenase 1 messenger RNA nitric oxide transcription factor Nrf2 antioxidant guanylate cyclase heme oxygenase 1 Nfe2l2 protein, mouse nitric oxide transcription factor Nrf2 adrenal cortex cell animal cell article cellular stress response controlled study enzyme induction intracellular signaling mouse nonhuman oxidative stress priority journal protein localization regulatory mechanism RNA degradation transcription initiation transient transfection adrenal gland animal cell culture cytology enzymology metabolism solubility Adrenal Glands Animals Antioxidants Cells, Cultured Guanylate Cyclase Heme Oxygenase-1 Mice NF-E2-Related Factor 2 Nitric Oxide Solubility Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulator of adrenal steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA/NO) on HO-1 induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by DETA/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2. © 2013 Elsevier Inc. All rights reserved. Fil:Astort, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Degese, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cymeryng, C.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10898603_v37_n1_p1_Astort |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cGMP Heme oxygenase 1 Nitric oxide Nuclear factor-erythroid 2-related factor (Nrf2) Soluble guanylate cyclase cyclic GMP dactinomycin diethylenetriamine guanylate cyclase guanylate cyclase inhibitor heme oxygenase 1 messenger RNA nitric oxide transcription factor Nrf2 antioxidant guanylate cyclase heme oxygenase 1 Nfe2l2 protein, mouse nitric oxide transcription factor Nrf2 adrenal cortex cell animal cell article cellular stress response controlled study enzyme induction intracellular signaling mouse nonhuman oxidative stress priority journal protein localization regulatory mechanism RNA degradation transcription initiation transient transfection adrenal gland animal cell culture cytology enzymology metabolism solubility Adrenal Glands Animals Antioxidants Cells, Cultured Guanylate Cyclase Heme Oxygenase-1 Mice NF-E2-Related Factor 2 Nitric Oxide Solubility |
| spellingShingle |
cGMP Heme oxygenase 1 Nitric oxide Nuclear factor-erythroid 2-related factor (Nrf2) Soluble guanylate cyclase cyclic GMP dactinomycin diethylenetriamine guanylate cyclase guanylate cyclase inhibitor heme oxygenase 1 messenger RNA nitric oxide transcription factor Nrf2 antioxidant guanylate cyclase heme oxygenase 1 Nfe2l2 protein, mouse nitric oxide transcription factor Nrf2 adrenal cortex cell animal cell article cellular stress response controlled study enzyme induction intracellular signaling mouse nonhuman oxidative stress priority journal protein localization regulatory mechanism RNA degradation transcription initiation transient transfection adrenal gland animal cell culture cytology enzymology metabolism solubility Adrenal Glands Animals Antioxidants Cells, Cultured Guanylate Cyclase Heme Oxygenase-1 Mice NF-E2-Related Factor 2 Nitric Oxide Solubility Astort, F. Mercau, M. Giordanino, E. Degese, M.S. Caldareri, L. Coso, O. Cymeryng, C.B. Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction |
| topic_facet |
cGMP Heme oxygenase 1 Nitric oxide Nuclear factor-erythroid 2-related factor (Nrf2) Soluble guanylate cyclase cyclic GMP dactinomycin diethylenetriamine guanylate cyclase guanylate cyclase inhibitor heme oxygenase 1 messenger RNA nitric oxide transcription factor Nrf2 antioxidant guanylate cyclase heme oxygenase 1 Nfe2l2 protein, mouse nitric oxide transcription factor Nrf2 adrenal cortex cell animal cell article cellular stress response controlled study enzyme induction intracellular signaling mouse nonhuman oxidative stress priority journal protein localization regulatory mechanism RNA degradation transcription initiation transient transfection adrenal gland animal cell culture cytology enzymology metabolism solubility Adrenal Glands Animals Antioxidants Cells, Cultured Guanylate Cyclase Heme Oxygenase-1 Mice NF-E2-Related Factor 2 Nitric Oxide Solubility |
| description |
Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulator of adrenal steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA/NO) on HO-1 induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by DETA/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2. © 2013 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Astort, F. Mercau, M. Giordanino, E. Degese, M.S. Caldareri, L. Coso, O. Cymeryng, C.B. |
| author_facet |
Astort, F. Mercau, M. Giordanino, E. Degese, M.S. Caldareri, L. Coso, O. Cymeryng, C.B. |
| author_sort |
Astort, F. |
| title |
Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction |
| title_short |
Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction |
| title_full |
Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction |
| title_fullStr |
Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction |
| title_full_unstemmed |
Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction |
| title_sort |
nitric oxide sets off an antioxidant response in adrenal cells: involvement of sgc and nrf2 in ho-1 induction |
| url |
http://hdl.handle.net/20.500.12110/paper_10898603_v37_n1_p1_Astort |
| work_keys_str_mv |
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