FRET imaging

Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of FRET te...

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Detalles Bibliográficos
Autores principales:	Jares-Erijman, E.A., Jovin, T.M.
Formato:	JOUR
Materias:	Cells Fluorescence Microscopic examination Photophysics Imaging techniques algorithm calculation cell conformation fluorescence resonance energy transfer imaging imaging system light optics phenomenology priority journal review Fluorescence Resonance Energy Transfer Fluorescent Dyes Microscopy, Fluorescence Models, Chemical Proteins Technology Assessment, Biomedical
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10870156_v21_n11_p1387_JaresErijman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_10870156_v21_n11_p1387_JaresErijman
record_format	dspace
spelling	todo:paper_10870156_v21_n11_p1387_JaresErijman2023-10-03T16:04:20Z FRET imaging Jares-Erijman, E.A. Jovin, T.M. Cells Fluorescence Microscopic examination Photophysics Imaging techniques algorithm calculation cell conformation fluorescence resonance energy transfer imaging imaging system light optics phenomenology priority journal review Fluorescence Resonance Energy Transfer Fluorescent Dyes Microscopy, Fluorescence Models, Chemical Proteins Technology Assessment, Biomedical Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of FRET techniques adapted to imaging systems, including new approaches proposed as suitable prospects for implementation. Applications extending from a single molecule to live cells will benefit from multidimensional microscopy techniques, particularly those adapted for optical sectioning and incorporating new algorithms for resolving the component contributions to images of complex molecular systems. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10870156_v21_n11_p1387_JaresErijman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cells Fluorescence Microscopic examination Photophysics Imaging techniques algorithm calculation cell conformation fluorescence resonance energy transfer imaging imaging system light optics phenomenology priority journal review Fluorescence Resonance Energy Transfer Fluorescent Dyes Microscopy, Fluorescence Models, Chemical Proteins Technology Assessment, Biomedical
spellingShingle	Cells Fluorescence Microscopic examination Photophysics Imaging techniques algorithm calculation cell conformation fluorescence resonance energy transfer imaging imaging system light optics phenomenology priority journal review Fluorescence Resonance Energy Transfer Fluorescent Dyes Microscopy, Fluorescence Models, Chemical Proteins Technology Assessment, Biomedical Jares-Erijman, E.A. Jovin, T.M. FRET imaging
topic_facet	Cells Fluorescence Microscopic examination Photophysics Imaging techniques algorithm calculation cell conformation fluorescence resonance energy transfer imaging imaging system light optics phenomenology priority journal review Fluorescence Resonance Energy Transfer Fluorescent Dyes Microscopy, Fluorescence Models, Chemical Proteins Technology Assessment, Biomedical
description	Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of FRET techniques adapted to imaging systems, including new approaches proposed as suitable prospects for implementation. Applications extending from a single molecule to live cells will benefit from multidimensional microscopy techniques, particularly those adapted for optical sectioning and incorporating new algorithms for resolving the component contributions to images of complex molecular systems.
format	JOUR
author	Jares-Erijman, E.A. Jovin, T.M.
author_facet	Jares-Erijman, E.A. Jovin, T.M.
author_sort	Jares-Erijman, E.A.
title	FRET imaging
title_short	FRET imaging
title_full	FRET imaging
title_fullStr	FRET imaging
title_full_unstemmed	FRET imaging
title_sort	fret imaging
url	http://hdl.handle.net/20.500.12110/paper_10870156_v21_n11_p1387_JaresErijman
work_keys_str_mv	AT jareserijmanea fretimaging AT jovintm fretimaging
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FRET imaging

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