Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats
The role of prolactin (Prl) in the regulation of Prl binding to its specific binding sites was studied in the Langerhans islets, adrenal gland and liver of adult ovariec tomized female rats. Animals were sc injected twice daily during 10 days with ovine Prl (1 mg/kg BW), sulpiride (30 mg/kg BW) and...
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todo:paper_10799893_v3_n6_p711_Tesone2023-10-03T16:03:44Z Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats Tesone, M. Luthy, I.A. Ladenheim, R.G. bromocriptine hormone receptor magnesium chloride prolactin prolactin i 125 prolactin receptor radioisotope sulpiride adrenal gland animal cell drug receptor binding endocrine system female genital system nonhuman ovariectomy pancreas islet pharmacokinetics rat Adrenal Glands Animal Bromocriptine Comparative Study Female Islets of Langerhans Liver Ovary Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, Prolactin Sulpiride Support, Non-U.S. Gov't The role of prolactin (Prl) in the regulation of Prl binding to its specific binding sites was studied in the Langerhans islets, adrenal gland and liver of adult ovariec tomized female rats. Animals were sc injected twice daily during 10 days with ovine Prl (1 mg/kg BW), sulpiride (30 mg/kg BW) and bromocriptine (3 mg/kg BW). At the end of the treatment period, the animals were killed and serum was collected for Prl assay. Total Prl binding sites were measured in the membrane fraction of tissue by desaturating the occupied membrane receptors in vitro with 4M MgCl2. Serum levels of Prl were significantly higher in sulpiride-treated animals, whereas bromocriptine administration rendered undetectable values. Prolactin and sulpiride treatment significantly reduced Prl binding to the adrenal gland and Langerhans islets, whereas it greatly increased Prl binding to the liver. On the other hand, bromocriptine increased Prl binding sites in the adrenal gland and Langerhans islets, but in the liver caused no apparent effect. The binding affinity (Ka) in each tissue remained unchanged under the different experimental conditions. In addition, the binding of Prl to pancreas islets membranes was lower in late pregnancy when compared with control rats. All of these data provide strong evidence in favor of a role for Prl in regulating the number of its own tissue binding sites. © 1983 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luthy, I.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ladenheim, R.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10799893_v3_n6_p711_Tesone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bromocriptine hormone receptor magnesium chloride prolactin prolactin i 125 prolactin receptor radioisotope sulpiride adrenal gland animal cell drug receptor binding endocrine system female genital system nonhuman ovariectomy pancreas islet pharmacokinetics rat Adrenal Glands Animal Bromocriptine Comparative Study Female Islets of Langerhans Liver Ovary Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, Prolactin Sulpiride Support, Non-U.S. Gov't |
| spellingShingle |
bromocriptine hormone receptor magnesium chloride prolactin prolactin i 125 prolactin receptor radioisotope sulpiride adrenal gland animal cell drug receptor binding endocrine system female genital system nonhuman ovariectomy pancreas islet pharmacokinetics rat Adrenal Glands Animal Bromocriptine Comparative Study Female Islets of Langerhans Liver Ovary Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, Prolactin Sulpiride Support, Non-U.S. Gov't Tesone, M. Luthy, I.A. Ladenheim, R.G. Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats |
| topic_facet |
bromocriptine hormone receptor magnesium chloride prolactin prolactin i 125 prolactin receptor radioisotope sulpiride adrenal gland animal cell drug receptor binding endocrine system female genital system nonhuman ovariectomy pancreas islet pharmacokinetics rat Adrenal Glands Animal Bromocriptine Comparative Study Female Islets of Langerhans Liver Ovary Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, Prolactin Sulpiride Support, Non-U.S. Gov't |
| description |
The role of prolactin (Prl) in the regulation of Prl binding to its specific binding sites was studied in the Langerhans islets, adrenal gland and liver of adult ovariec tomized female rats. Animals were sc injected twice daily during 10 days with ovine Prl (1 mg/kg BW), sulpiride (30 mg/kg BW) and bromocriptine (3 mg/kg BW). At the end of the treatment period, the animals were killed and serum was collected for Prl assay. Total Prl binding sites were measured in the membrane fraction of tissue by desaturating the occupied membrane receptors in vitro with 4M MgCl2. Serum levels of Prl were significantly higher in sulpiride-treated animals, whereas bromocriptine administration rendered undetectable values. Prolactin and sulpiride treatment significantly reduced Prl binding to the adrenal gland and Langerhans islets, whereas it greatly increased Prl binding to the liver. On the other hand, bromocriptine increased Prl binding sites in the adrenal gland and Langerhans islets, but in the liver caused no apparent effect. The binding affinity (Ka) in each tissue remained unchanged under the different experimental conditions. In addition, the binding of Prl to pancreas islets membranes was lower in late pregnancy when compared with control rats. All of these data provide strong evidence in favor of a role for Prl in regulating the number of its own tissue binding sites. © 1983 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| format |
JOUR |
| author |
Tesone, M. Luthy, I.A. Ladenheim, R.G. |
| author_facet |
Tesone, M. Luthy, I.A. Ladenheim, R.G. |
| author_sort |
Tesone, M. |
| title |
Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats |
| title_short |
Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats |
| title_full |
Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats |
| title_fullStr |
Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats |
| title_full_unstemmed |
Prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats |
| title_sort |
prolactin regulation of prolactin binding sites in pancreatic islets and adrenal glands of ovariectomized rats |
| url |
http://hdl.handle.net/20.500.12110/paper_10799893_v3_n6_p711_Tesone |
| work_keys_str_mv |
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| _version_ |
1807315673920045056 |