Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highl...

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Detalles Bibliográficos
Autores principales: Fernanda Ledda, M., Adris, S., Bravo, A.I., Kairiyama, C., Bover, L., Chernajovsky, Y., Mordoh, J., Podhajcer, O.L.
Formato: JOUR
Materias:
complementary rna
glycoprotein
osteonectin
animal experiment
article
cancer inhibition
cancer invasion
controlled study
human
human cell
melanoma cell
metastasis
mouse
nonhuman
priority journal
Animals
Cell Adhesion
Cell Division
Cell Movement
Down-Regulation
Gene Expression Regulation, Neoplastic
Humans
Melanoma
Melanoma, Experimental
Mice
Oligonucleotides, Antisense
Osteonectin
Transfection
Tumor Cells, Cultured
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development.

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