Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highl...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda |
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todo:paper_10788956_v3_n2_p171_FernandaLedda2023-10-03T16:03:42Z Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells Fernanda Ledda, M. Adris, S. Bravo, A.I. Kairiyama, C. Bover, L. Chernajovsky, Y. Mordoh, J. Podhajcer, O.L. complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development. Fil:Fernanda Ledda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bover, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Podhajcer, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia |
| spellingShingle |
complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia Fernanda Ledda, M. Adris, S. Bravo, A.I. Kairiyama, C. Bover, L. Chernajovsky, Y. Mordoh, J. Podhajcer, O.L. Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells |
| topic_facet |
complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia |
| description |
Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development. |
| format |
JOUR |
| author |
Fernanda Ledda, M. Adris, S. Bravo, A.I. Kairiyama, C. Bover, L. Chernajovsky, Y. Mordoh, J. Podhajcer, O.L. |
| author_facet |
Fernanda Ledda, M. Adris, S. Bravo, A.I. Kairiyama, C. Bover, L. Chernajovsky, Y. Mordoh, J. Podhajcer, O.L. |
| author_sort |
Fernanda Ledda, M. |
| title |
Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells |
| title_short |
Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells |
| title_full |
Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells |
| title_fullStr |
Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells |
| title_full_unstemmed |
Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells |
| title_sort |
suppression of sparc expression by antisense rna abrogates the tumorigenicity of human melanoma cells |
| url |
http://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda |
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| _version_ |
1807321555071401984 |