Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer

Purpose: Targeted therapies that use the signaling path-characterize the function of human FKBP7 and explore its ways involved in prostate cancer are required to overcome role in cancer. We discovered that FKBP7 was upregulated chemoresistance and improve treatment outcomes for men. in human prostat...

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Autores principales:	Garrido, M.F., Martin, N.J.-P., Bertrand, M., Gaudin, C., Commo, F.E., Kalaany, N.E., Nakouzi, N.A., Fazli, L., Nery, E.D., Camonis, J., Perez, F., Lerondel, S., Le Pape, A., Compagno, D., Gleave, M., Loriot, Y., Desaubry, L., Vagner, S., Fizazi, K., Chauchereau, A.
Formato:	JOUR
Materias:	cabazitaxel docetaxel initiation factor 4F initiation factor 4FG peptidylprolyl isomerase peptidylprolyl isomerase FKBP7 short hairpin RNA small interfering RNA taxane derivative unclassified drug animal experiment animal model animal tissue Article cancer resistance carcinogenicity cell proliferation cell survival controlled study drug targeting enzyme activity gene expression regulation gene knockdown gene silencing genetic transfection human human cell human tissue in vitro study in vivo study intracellular signaling male mass spectrometry nonhuman priority journal prostate cancer cell line protein expression protein protein interaction tissue microarray translation initiation translation regulation tumor xenograft upregulation
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10780432_v25_n2_p710_Garrido
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_10780432_v25_n2_p710_Garrido
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spelling	todo:paper_10780432_v25_n2_p710_Garrido2023-10-03T16:03:37Z Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer Garrido, M.F. Martin, N.J.-P. Bertrand, M. Gaudin, C. Commo, F.E. Kalaany, N.E. Nakouzi, N.A. Fazli, L. Nery, E.D. Camonis, J. Perez, F. Lerondel, S. Le Pape, A. Compagno, D. Gleave, M. Loriot, Y. Desaubry, L. Vagner, S. Fizazi, K. Chauchereau, A. cabazitaxel docetaxel initiation factor 4F initiation factor 4FG peptidylprolyl isomerase peptidylprolyl isomerase FKBP7 short hairpin RNA small interfering RNA taxane derivative unclassified drug animal experiment animal model animal tissue Article cancer resistance carcinogenicity cell proliferation cell survival controlled study drug targeting enzyme activity gene expression regulation gene knockdown gene silencing genetic transfection human human cell human tissue in vitro study in vivo study intracellular signaling male mass spectrometry nonhuman priority journal prostate cancer cell line protein expression protein protein interaction tissue microarray translation initiation translation regulation tumor xenograft upregulation Purpose: Targeted therapies that use the signaling path-characterize the function of human FKBP7 and explore its ways involved in prostate cancer are required to overcome role in cancer. We discovered that FKBP7 was upregulated chemoresistance and improve treatment outcomes for men. in human prostate cancers and its expression correlated Molecular chaperones play a key role in the regulation of with the recurrence observed in patients receiving doce-protein homeostasis and are potential targets for overcom-taxel. FKBP7 silencing showed that FKBP7 is required to ing chemoresistance. maintain the growth of chemoresistant cell lines and Experimental Design: We established 4 chemoresistant chemoresistant tumors in mice. Mass spectrometry analysis prostate cancer cell lines and used image-based high-content revealed that FKBP7 interacts with eIF4G, a component of siRNA functional screening, based on gene-expression signa-the eIF4F translation initiation complex, to mediate the ture, to explore mechanisms of chemoresistance and identify survival of chemoresistant cells. Using small-molecule new potential targets with potential roles in taxane resistance. inhibitors of eIF4A, the RNA helicase component of The functional role of a new target was assessed by in vitro and eIF4F, we were able to kill docetaxel- and cabazitaxel-in vivo silencing, and mass spectrometry analysis was used to resistant cells. identify its downstream effectors. Conclusions: Targeting FKBP7 or the eIF4G-containing Results: We identified FKBP7, a prolyl-peptidyl isomer-eIF4F translation initiation complex could be novel thera-ase overexpressed in docetaxel-resistant and in cabazitaxel-peutic strategies to eradicate taxane-resistant prostate cancer resistant prostate cancer cells. This is the first study to cells. © 2018 American Association for Cancer Research. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10780432_v25_n2_p710_Garrido
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cabazitaxel docetaxel initiation factor 4F initiation factor 4FG peptidylprolyl isomerase peptidylprolyl isomerase FKBP7 short hairpin RNA small interfering RNA taxane derivative unclassified drug animal experiment animal model animal tissue Article cancer resistance carcinogenicity cell proliferation cell survival controlled study drug targeting enzyme activity gene expression regulation gene knockdown gene silencing genetic transfection human human cell human tissue in vitro study in vivo study intracellular signaling male mass spectrometry nonhuman priority journal prostate cancer cell line protein expression protein protein interaction tissue microarray translation initiation translation regulation tumor xenograft upregulation
spellingShingle	cabazitaxel docetaxel initiation factor 4F initiation factor 4FG peptidylprolyl isomerase peptidylprolyl isomerase FKBP7 short hairpin RNA small interfering RNA taxane derivative unclassified drug animal experiment animal model animal tissue Article cancer resistance carcinogenicity cell proliferation cell survival controlled study drug targeting enzyme activity gene expression regulation gene knockdown gene silencing genetic transfection human human cell human tissue in vitro study in vivo study intracellular signaling male mass spectrometry nonhuman priority journal prostate cancer cell line protein expression protein protein interaction tissue microarray translation initiation translation regulation tumor xenograft upregulation Garrido, M.F. Martin, N.J.-P. Bertrand, M. Gaudin, C. Commo, F.E. Kalaany, N.E. Nakouzi, N.A. Fazli, L. Nery, E.D. Camonis, J. Perez, F. Lerondel, S. Le Pape, A. Compagno, D. Gleave, M. Loriot, Y. Desaubry, L. Vagner, S. Fizazi, K. Chauchereau, A. Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer
topic_facet	cabazitaxel docetaxel initiation factor 4F initiation factor 4FG peptidylprolyl isomerase peptidylprolyl isomerase FKBP7 short hairpin RNA small interfering RNA taxane derivative unclassified drug animal experiment animal model animal tissue Article cancer resistance carcinogenicity cell proliferation cell survival controlled study drug targeting enzyme activity gene expression regulation gene knockdown gene silencing genetic transfection human human cell human tissue in vitro study in vivo study intracellular signaling male mass spectrometry nonhuman priority journal prostate cancer cell line protein expression protein protein interaction tissue microarray translation initiation translation regulation tumor xenograft upregulation
description	Purpose: Targeted therapies that use the signaling path-characterize the function of human FKBP7 and explore its ways involved in prostate cancer are required to overcome role in cancer. We discovered that FKBP7 was upregulated chemoresistance and improve treatment outcomes for men. in human prostate cancers and its expression correlated Molecular chaperones play a key role in the regulation of with the recurrence observed in patients receiving doce-protein homeostasis and are potential targets for overcom-taxel. FKBP7 silencing showed that FKBP7 is required to ing chemoresistance. maintain the growth of chemoresistant cell lines and Experimental Design: We established 4 chemoresistant chemoresistant tumors in mice. Mass spectrometry analysis prostate cancer cell lines and used image-based high-content revealed that FKBP7 interacts with eIF4G, a component of siRNA functional screening, based on gene-expression signa-the eIF4F translation initiation complex, to mediate the ture, to explore mechanisms of chemoresistance and identify survival of chemoresistant cells. Using small-molecule new potential targets with potential roles in taxane resistance. inhibitors of eIF4A, the RNA helicase component of The functional role of a new target was assessed by in vitro and eIF4F, we were able to kill docetaxel- and cabazitaxel-in vivo silencing, and mass spectrometry analysis was used to resistant cells. identify its downstream effectors. Conclusions: Targeting FKBP7 or the eIF4G-containing Results: We identified FKBP7, a prolyl-peptidyl isomer-eIF4F translation initiation complex could be novel thera-ase overexpressed in docetaxel-resistant and in cabazitaxel-peutic strategies to eradicate taxane-resistant prostate cancer resistant prostate cancer cells. This is the first study to cells. © 2018 American Association for Cancer Research.
format	JOUR
author	Garrido, M.F. Martin, N.J.-P. Bertrand, M. Gaudin, C. Commo, F.E. Kalaany, N.E. Nakouzi, N.A. Fazli, L. Nery, E.D. Camonis, J. Perez, F. Lerondel, S. Le Pape, A. Compagno, D. Gleave, M. Loriot, Y. Desaubry, L. Vagner, S. Fizazi, K. Chauchereau, A.
author_facet	Garrido, M.F. Martin, N.J.-P. Bertrand, M. Gaudin, C. Commo, F.E. Kalaany, N.E. Nakouzi, N.A. Fazli, L. Nery, E.D. Camonis, J. Perez, F. Lerondel, S. Le Pape, A. Compagno, D. Gleave, M. Loriot, Y. Desaubry, L. Vagner, S. Fizazi, K. Chauchereau, A.
author_sort	Garrido, M.F.
title	Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer
title_short	Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer
title_full	Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer
title_fullStr	Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer
title_full_unstemmed	Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer
title_sort	regulation of eif4f translation initiation complex by the peptidyl prolyl isomerase fkbp7 in taxane-resistant prostate cancer
url	http://hdl.handle.net/20.500.12110/paper_10780432_v25_n2_p710_Garrido
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Regulation of eIF4F Translation Initiation Complex by the Peptidyl Prolyl Isomerase FKBP7 in Taxane-resistant Prostate Cancer

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