Application of Z-sinapinic matrix in peptide MALDI-MS analysis

Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite a...

Descripción completa

Detalles Bibliográficos
Autores principales: Salum, M.L., Giudicessi, S.L., Schmidt De León, T., Camperi, S.A., Erra-Balsells, R.
Formato: JOUR
Materias:
CHCA clusters
MALDI-MS
peptides
Z-sinapinic acid
alpha cyano 4 hydroxycinnamic acid
angiotensin I
angiotensin II
bradykinin
peptide
sinapic acid
alpha-cyano-4-hydroxycinnamate
cinnamic acid
cinnamic acid derivative
coumaric acid
limit of detection
matrix-assisted laser desorption-ionization mass spectrometry
molecular model
Note
peptide analysis
peptide synthesis
priority journal
amino acid sequence
chemical phenomena
chemistry
Amino Acid Sequence
Cinnamates
Coumaric Acids
Hydrophobic and Hydrophilic Interactions
Limit of Detection
Models, Molecular
Peptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10765174_v52_n3_p182_Salum
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_10765174_v52_n3_p182_Salum
record_format dspace
spelling todo:paper_10765174_v52_n3_p182_Salum2023-10-03T16:03:27Z Application of Z-sinapinic matrix in peptide MALDI-MS analysis Salum, M.L. Giudicessi, S.L. Schmidt De León, T. Camperi, S.A. Erra-Balsells, R. CHCA clusters MALDI-MS peptides Z-sinapinic acid alpha cyano 4 hydroxycinnamic acid angiotensin I angiotensin II bradykinin peptide sinapic acid alpha-cyano-4-hydroxycinnamate cinnamic acid cinnamic acid derivative coumaric acid peptide sinapic acid limit of detection matrix-assisted laser desorption-ionization mass spectrometry molecular model Note peptide analysis peptide synthesis priority journal amino acid sequence chemical phenomena chemistry matrix-assisted laser desorption-ionization mass spectrometry Amino Acid Sequence Cinnamates Coumaric Acids Hydrophobic and Hydrophilic Interactions Limit of Detection Models, Molecular Peptides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd. Fil:Giudicessi, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10765174_v52_n3_p182_Salum
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic CHCA clusters
MALDI-MS
peptides
Z-sinapinic acid
alpha cyano 4 hydroxycinnamic acid
angiotensin I
angiotensin II
bradykinin
peptide
sinapic acid
alpha-cyano-4-hydroxycinnamate
cinnamic acid
cinnamic acid derivative
coumaric acid
peptide
sinapic acid
limit of detection
matrix-assisted laser desorption-ionization mass spectrometry
molecular model
Note
peptide analysis
peptide synthesis
priority journal
amino acid sequence
chemical phenomena
chemistry
matrix-assisted laser desorption-ionization mass spectrometry
Amino Acid Sequence
Cinnamates
Coumaric Acids
Hydrophobic and Hydrophilic Interactions
Limit of Detection
Models, Molecular
Peptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
spellingShingle CHCA clusters
MALDI-MS
peptides
Z-sinapinic acid
alpha cyano 4 hydroxycinnamic acid
angiotensin I
angiotensin II
bradykinin
peptide
sinapic acid
alpha-cyano-4-hydroxycinnamate
cinnamic acid
cinnamic acid derivative
coumaric acid
peptide
sinapic acid
limit of detection
matrix-assisted laser desorption-ionization mass spectrometry
molecular model
Note
peptide analysis
peptide synthesis
priority journal
amino acid sequence
chemical phenomena
chemistry
matrix-assisted laser desorption-ionization mass spectrometry
Amino Acid Sequence
Cinnamates
Coumaric Acids
Hydrophobic and Hydrophilic Interactions
Limit of Detection
Models, Molecular
Peptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Salum, M.L.
Giudicessi, S.L.
Schmidt De León, T.
Camperi, S.A.
Erra-Balsells, R.
Application of Z-sinapinic matrix in peptide MALDI-MS analysis
topic_facet CHCA clusters
MALDI-MS
peptides
Z-sinapinic acid
alpha cyano 4 hydroxycinnamic acid
angiotensin I
angiotensin II
bradykinin
peptide
sinapic acid
alpha-cyano-4-hydroxycinnamate
cinnamic acid
cinnamic acid derivative
coumaric acid
peptide
sinapic acid
limit of detection
matrix-assisted laser desorption-ionization mass spectrometry
molecular model
Note
peptide analysis
peptide synthesis
priority journal
amino acid sequence
chemical phenomena
chemistry
matrix-assisted laser desorption-ionization mass spectrometry
Amino Acid Sequence
Cinnamates
Coumaric Acids
Hydrophobic and Hydrophilic Interactions
Limit of Detection
Models, Molecular
Peptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
description Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.
format JOUR
author Salum, M.L.
Giudicessi, S.L.
Schmidt De León, T.
Camperi, S.A.
Erra-Balsells, R.
author_facet Salum, M.L.
Giudicessi, S.L.
Schmidt De León, T.
Camperi, S.A.
Erra-Balsells, R.
author_sort Salum, M.L.
title Application of Z-sinapinic matrix in peptide MALDI-MS analysis
title_short Application of Z-sinapinic matrix in peptide MALDI-MS analysis
title_full Application of Z-sinapinic matrix in peptide MALDI-MS analysis
title_fullStr Application of Z-sinapinic matrix in peptide MALDI-MS analysis
title_full_unstemmed Application of Z-sinapinic matrix in peptide MALDI-MS analysis
title_sort application of z-sinapinic matrix in peptide maldi-ms analysis
url http://hdl.handle.net/20.500.12110/paper_10765174_v52_n3_p182_Salum
work_keys_str_mv AT salumml applicationofzsinapinicmatrixinpeptidemaldimsanalysis
AT giudicessisl applicationofzsinapinicmatrixinpeptidemaldimsanalysis
AT schmidtdeleont applicationofzsinapinicmatrixinpeptidemaldimsanalysis
AT camperisa applicationofzsinapinicmatrixinpeptidemaldimsanalysis
AT errabalsellsr applicationofzsinapinicmatrixinpeptidemaldimsanalysis
_version_ 1807320473422266368

Ejemplares similares