Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus

Activation of the cAMP pathway was found to be implicated in the memory process. In the context-signal learning paradigm of the crab Chasmagnathus, the protein kinase (PKA) activator Sp-5,6-DCl-cBIMPS facilitated long-term memory (LTM) induced by spaced training while the PKA inhibitor 8-chloroadeno...

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Autores principales: Locatelli, F., Maldonado, H., Romano, A.
Formato: JOUR
Materias:
cyclic AMP dependent protein kinase
cyclic AMP dependent protein kinase inhibitor
immunoglobulin enhancer binding protein
amnesia
animal experiment
article
concentration response
controlled study
crab
enzyme activation
enzyme activity
enzyme inhibition
long term memory
male
memory consolidation
nonhuman
task performance
training
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10747427_v77_n2_p234_Locatelli
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_10747427_v77_n2_p234_Locatelli
record_format dspace
spelling todo:paper_10747427_v77_n2_p234_Locatelli2023-10-03T16:03:04Z Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus Locatelli, F. Maldonado, H. Romano, A. cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor immunoglobulin enhancer binding protein amnesia animal experiment article concentration response controlled study crab enzyme activation enzyme activity enzyme inhibition long term memory male memory consolidation nonhuman task performance training Activation of the cAMP pathway was found to be implicated in the memory process. In the context-signal learning paradigm of the crab Chasmagnathus, the protein kinase (PKA) activator Sp-5,6-DCl-cBIMPS facilitated long-term memory (LTM) induced by spaced training while the PKA inhibitor 8-chloroadenosine-3′, 5′-monophosphorothioate, Rp-isomer (Rp-8-Cl-cAMPS) produced amnesia. In the present report the effect of the PKA inhibitor on long-term retention was assessed when administered (systemic injection of 2 μg/animal) at various times after training. According to previous results obtained with a lower dose, retention is impaired when the drug is administered immediately pretraining. An effect on acquisition was ruled out considering that the drug did not affect the performance during training. On the contrary, no effect of the PKA inhibitor was found with an immediately posttraining injection and amnesia was observed only when training was shortened from 15 to 12 trials (training duration from 45 to 36 min). At 2 and 12 h posttraining Rp-8-Cl-cAMPS injection failed to impair retention, but amnesia was found when the drug was injected at 4 and 8 h after training. In order to assess a possible effect of the drug in retrieval, the PKA inhibitor was administered 15 min before testing, and no amnestic effect was observed. These results suggest that two phases of PKA activity are required during consolidation of LTM, one during training and the other between 4 and 8 h after training. The link between these two periods of PKA activation and the two phases of the transcription factor NF-κB activation previously found in this model, as well as the similar time course found in rodents, is discussed. An amnestic effect of the drug was not found when administered immediately before a massed training protocol that yielded an intermediate-term memory, suggesting that in this type of memory PKA activation is not required. © 2002 Elsevier Science (USA). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10747427_v77_n2_p234_Locatelli
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cyclic AMP dependent protein kinase
cyclic AMP dependent protein kinase inhibitor
immunoglobulin enhancer binding protein
amnesia
animal experiment
article
concentration response
controlled study
crab
enzyme activation
enzyme activity
enzyme inhibition
long term memory
male
memory consolidation
nonhuman
task performance
training
spellingShingle cyclic AMP dependent protein kinase
cyclic AMP dependent protein kinase inhibitor
immunoglobulin enhancer binding protein
amnesia
animal experiment
article
concentration response
controlled study
crab
enzyme activation
enzyme activity
enzyme inhibition
long term memory
male
memory consolidation
nonhuman
task performance
training
Locatelli, F.
Maldonado, H.
Romano, A.
Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus
topic_facet cyclic AMP dependent protein kinase
cyclic AMP dependent protein kinase inhibitor
immunoglobulin enhancer binding protein
amnesia
animal experiment
article
concentration response
controlled study
crab
enzyme activation
enzyme activity
enzyme inhibition
long term memory
male
memory consolidation
nonhuman
task performance
training
description Activation of the cAMP pathway was found to be implicated in the memory process. In the context-signal learning paradigm of the crab Chasmagnathus, the protein kinase (PKA) activator Sp-5,6-DCl-cBIMPS facilitated long-term memory (LTM) induced by spaced training while the PKA inhibitor 8-chloroadenosine-3′, 5′-monophosphorothioate, Rp-isomer (Rp-8-Cl-cAMPS) produced amnesia. In the present report the effect of the PKA inhibitor on long-term retention was assessed when administered (systemic injection of 2 μg/animal) at various times after training. According to previous results obtained with a lower dose, retention is impaired when the drug is administered immediately pretraining. An effect on acquisition was ruled out considering that the drug did not affect the performance during training. On the contrary, no effect of the PKA inhibitor was found with an immediately posttraining injection and amnesia was observed only when training was shortened from 15 to 12 trials (training duration from 45 to 36 min). At 2 and 12 h posttraining Rp-8-Cl-cAMPS injection failed to impair retention, but amnesia was found when the drug was injected at 4 and 8 h after training. In order to assess a possible effect of the drug in retrieval, the PKA inhibitor was administered 15 min before testing, and no amnestic effect was observed. These results suggest that two phases of PKA activity are required during consolidation of LTM, one during training and the other between 4 and 8 h after training. The link between these two periods of PKA activation and the two phases of the transcription factor NF-κB activation previously found in this model, as well as the similar time course found in rodents, is discussed. An amnestic effect of the drug was not found when administered immediately before a massed training protocol that yielded an intermediate-term memory, suggesting that in this type of memory PKA activation is not required. © 2002 Elsevier Science (USA).
format JOUR
author Locatelli, F.
Maldonado, H.
Romano, A.
author_facet Locatelli, F.
Maldonado, H.
Romano, A.
author_sort Locatelli, F.
title Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus
title_short Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus
title_full Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus
title_fullStr Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus
title_full_unstemmed Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus
title_sort two critical periods for camp-dependent protein kinase activity during long-term memory consolidation in the crab chasmagnathus
url http://hdl.handle.net/20.500.12110/paper_10747427_v77_n2_p234_Locatelli
work_keys_str_mv AT locatellif twocriticalperiodsforcampdependentproteinkinaseactivityduringlongtermmemoryconsolidationinthecrabchasmagnathus
AT maldonadoh twocriticalperiodsforcampdependentproteinkinaseactivityduringlongtermmemoryconsolidationinthecrabchasmagnathus
AT romanoa twocriticalperiodsforcampdependentproteinkinaseactivityduringlongtermmemoryconsolidationinthecrabchasmagnathus
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