Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata
The aim of the present study was to analyze the surface expression of the NMDA-like receptors during the consolidation of contextual learning in the crab Neohelice granulata. Memory storage is based on alterations in the strength of synaptic connections between neurons. The glutamatergic synapses un...
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todo:paper_10720502_v23_n8_p427_Hepp2023-10-03T16:02:44Z Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata Hepp, Y. Salles, A. Carbo-Tano, M. Pedreira, M.E. Freudenthal, R. bicuculline n methyl dextro aspartic acid receptor 4 aminobutyric acid A receptor blocking agent bicuculline n methyl dextro aspartic acid receptor adult animal experiment Article cell membrane cell migration controlled study crab down regulation long term memory male memory consolidation Neohelice granulata nerve cell plasticity nonhuman priority journal protein expression regulatory mechanism surface property thoracic ganglion upregulation animal association Brachyura brain drug effects learning memory consolidation metabolism physiology protein subunit Animals Bicuculline Brachyura Brain Cues GABA-A Receptor Antagonists Learning Male Memory Consolidation Protein Subunits Receptors, N-Methyl-D-Aspartate The aim of the present study was to analyze the surface expression of the NMDA-like receptors during the consolidation of contextual learning in the crab Neohelice granulata. Memory storage is based on alterations in the strength of synaptic connections between neurons. The glutamatergic synapses undergo various forms of N-methyl-D aspartate receptor (NMDAR)-dependent changes in strength, a process that affects the abundance of other receptors at the synapse and underlies some forms of learning and memory. Here we propose a direct regulation of the NMDAR. Changes in NMDAR's functionality might be induced by the modification of the subunit's expression or cellular trafficking. This trafficking does not only include NMDAR's movement between synaptic and extra-synaptic localizations but also the cycling between intracellular compartments and the plasma membrane, a process called surface expression. Consolidation of contextual learning affects the surface expression of the receptor without affecting its general expression. The surface expression of the GluN1 subunit of the NMDAR is down-regulated immediately after training, up-regulated 3 h after training and returns to nave and control levels 24 h after training. The changes in NMDAR surface expression observed in the central brain are not seen in the thoracic ganglion. A similar increment in surface expression of GluN1 in the central brain is observed 3 h after administration of the competitive GABAA receptor antagonist, bicuculline. These consolidation changes are part of a plasticity event that first, during the down-regulation, stabilizes the trace and later, at 3-h post-training, changes the threshold for synapse activation. Fil:Hepp, Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salles, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carbo-Tano, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pedreira, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Freudenthal, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10720502_v23_n8_p427_Hepp |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bicuculline n methyl dextro aspartic acid receptor 4 aminobutyric acid A receptor blocking agent bicuculline n methyl dextro aspartic acid receptor adult animal experiment Article cell membrane cell migration controlled study crab down regulation long term memory male memory consolidation Neohelice granulata nerve cell plasticity nonhuman priority journal protein expression regulatory mechanism surface property thoracic ganglion upregulation animal association Brachyura brain drug effects learning memory consolidation metabolism physiology protein subunit Animals Bicuculline Brachyura Brain Cues GABA-A Receptor Antagonists Learning Male Memory Consolidation Protein Subunits Receptors, N-Methyl-D-Aspartate |
| spellingShingle |
bicuculline n methyl dextro aspartic acid receptor 4 aminobutyric acid A receptor blocking agent bicuculline n methyl dextro aspartic acid receptor adult animal experiment Article cell membrane cell migration controlled study crab down regulation long term memory male memory consolidation Neohelice granulata nerve cell plasticity nonhuman priority journal protein expression regulatory mechanism surface property thoracic ganglion upregulation animal association Brachyura brain drug effects learning memory consolidation metabolism physiology protein subunit Animals Bicuculline Brachyura Brain Cues GABA-A Receptor Antagonists Learning Male Memory Consolidation Protein Subunits Receptors, N-Methyl-D-Aspartate Hepp, Y. Salles, A. Carbo-Tano, M. Pedreira, M.E. Freudenthal, R. Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata |
| topic_facet |
bicuculline n methyl dextro aspartic acid receptor 4 aminobutyric acid A receptor blocking agent bicuculline n methyl dextro aspartic acid receptor adult animal experiment Article cell membrane cell migration controlled study crab down regulation long term memory male memory consolidation Neohelice granulata nerve cell plasticity nonhuman priority journal protein expression regulatory mechanism surface property thoracic ganglion upregulation animal association Brachyura brain drug effects learning memory consolidation metabolism physiology protein subunit Animals Bicuculline Brachyura Brain Cues GABA-A Receptor Antagonists Learning Male Memory Consolidation Protein Subunits Receptors, N-Methyl-D-Aspartate |
| description |
The aim of the present study was to analyze the surface expression of the NMDA-like receptors during the consolidation of contextual learning in the crab Neohelice granulata. Memory storage is based on alterations in the strength of synaptic connections between neurons. The glutamatergic synapses undergo various forms of N-methyl-D aspartate receptor (NMDAR)-dependent changes in strength, a process that affects the abundance of other receptors at the synapse and underlies some forms of learning and memory. Here we propose a direct regulation of the NMDAR. Changes in NMDAR's functionality might be induced by the modification of the subunit's expression or cellular trafficking. This trafficking does not only include NMDAR's movement between synaptic and extra-synaptic localizations but also the cycling between intracellular compartments and the plasma membrane, a process called surface expression. Consolidation of contextual learning affects the surface expression of the receptor without affecting its general expression. The surface expression of the GluN1 subunit of the NMDAR is down-regulated immediately after training, up-regulated 3 h after training and returns to nave and control levels 24 h after training. The changes in NMDAR surface expression observed in the central brain are not seen in the thoracic ganglion. A similar increment in surface expression of GluN1 in the central brain is observed 3 h after administration of the competitive GABAA receptor antagonist, bicuculline. These consolidation changes are part of a plasticity event that first, during the down-regulation, stabilizes the trace and later, at 3-h post-training, changes the threshold for synapse activation. |
| format |
JOUR |
| author |
Hepp, Y. Salles, A. Carbo-Tano, M. Pedreira, M.E. Freudenthal, R. |
| author_facet |
Hepp, Y. Salles, A. Carbo-Tano, M. Pedreira, M.E. Freudenthal, R. |
| author_sort |
Hepp, Y. |
| title |
Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata |
| title_short |
Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata |
| title_full |
Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata |
| title_fullStr |
Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata |
| title_full_unstemmed |
Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata |
| title_sort |
surface expression of nmda receptor changes during memory consolidation in the crab neohelice granulata |
| url |
http://hdl.handle.net/20.500.12110/paper_10720502_v23_n8_p427_Hepp |
| work_keys_str_mv |
AT heppy surfaceexpressionofnmdareceptorchangesduringmemoryconsolidationinthecrabneohelicegranulata AT sallesa surfaceexpressionofnmdareceptorchangesduringmemoryconsolidationinthecrabneohelicegranulata AT carbotanom surfaceexpressionofnmdareceptorchangesduringmemoryconsolidationinthecrabneohelicegranulata AT pedreirame surfaceexpressionofnmdareceptorchangesduringmemoryconsolidationinthecrabneohelicegranulata AT freudenthalr surfaceexpressionofnmdareceptorchangesduringmemoryconsolidationinthecrabneohelicegranulata |
| _version_ |
1807320405221834752 |