Human sperm β-glucuronidase is poorly extractable by triton X-100

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A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1...

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Detalles Bibliográficos
Autores principales: Brandelli, A., Miranda, P.V., Tezón, J.G.
Formato: JOUR
Materias:
Cytoskeleton
Detergent
Enzyme
Glycosidase
Sperm
beta glucuronidase
colchicine
cytochalasin B
deoxyribonuclease I
potassium chloride
triton x 100
article
controlled study
enzyme activity
human
human cell
male
normal human
protein isolation
solubilization
sperm
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10656995_v20_n5_p351_Brandelli
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1 M KCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3 M KCl or DNAse I treatment. Results demonstrate that β-glucuronidase is tightly associated to the Triton X-100 resistant fraction.

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