Human sperm β-glucuronidase is poorly extractable by triton X-100
A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10656995_v20_n5_p351_Brandelli |
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todo:paper_10656995_v20_n5_p351_Brandelli2023-10-03T16:02:04Z Human sperm β-glucuronidase is poorly extractable by triton X-100 Brandelli, A. Miranda, P.V. Tezón, J.G. Cytoskeleton Detergent Enzyme Glycosidase Sperm beta glucuronidase colchicine cytochalasin B deoxyribonuclease I potassium chloride triton x 100 article controlled study enzyme activity human human cell male normal human protein isolation solubilization sperm A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1 M KCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3 M KCl or DNAse I treatment. Results demonstrate that β-glucuronidase is tightly associated to the Triton X-100 resistant fraction. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10656995_v20_n5_p351_Brandelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cytoskeleton Detergent Enzyme Glycosidase Sperm beta glucuronidase colchicine cytochalasin B deoxyribonuclease I potassium chloride triton x 100 article controlled study enzyme activity human human cell male normal human protein isolation solubilization sperm |
| spellingShingle |
Cytoskeleton Detergent Enzyme Glycosidase Sperm beta glucuronidase colchicine cytochalasin B deoxyribonuclease I potassium chloride triton x 100 article controlled study enzyme activity human human cell male normal human protein isolation solubilization sperm Brandelli, A. Miranda, P.V. Tezón, J.G. Human sperm β-glucuronidase is poorly extractable by triton X-100 |
| topic_facet |
Cytoskeleton Detergent Enzyme Glycosidase Sperm beta glucuronidase colchicine cytochalasin B deoxyribonuclease I potassium chloride triton x 100 article controlled study enzyme activity human human cell male normal human protein isolation solubilization sperm |
| description |
A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1 M KCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3 M KCl or DNAse I treatment. Results demonstrate that β-glucuronidase is tightly associated to the Triton X-100 resistant fraction. |
| format |
JOUR |
| author |
Brandelli, A. Miranda, P.V. Tezón, J.G. |
| author_facet |
Brandelli, A. Miranda, P.V. Tezón, J.G. |
| author_sort |
Brandelli, A. |
| title |
Human sperm β-glucuronidase is poorly extractable by triton X-100 |
| title_short |
Human sperm β-glucuronidase is poorly extractable by triton X-100 |
| title_full |
Human sperm β-glucuronidase is poorly extractable by triton X-100 |
| title_fullStr |
Human sperm β-glucuronidase is poorly extractable by triton X-100 |
| title_full_unstemmed |
Human sperm β-glucuronidase is poorly extractable by triton X-100 |
| title_sort |
human sperm β-glucuronidase is poorly extractable by triton x-100 |
| url |
http://hdl.handle.net/20.500.12110/paper_10656995_v20_n5_p351_Brandelli |
| work_keys_str_mv |
AT brandellia humanspermbglucuronidaseispoorlyextractablebytritonx100 AT mirandapv humanspermbglucuronidaseispoorlyextractablebytritonx100 AT tezonjg humanspermbglucuronidaseispoorlyextractablebytritonx100 |
| _version_ |
1807317922188623872 |