Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p27_Piuri |
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todo:paper_10643745_v1898_n_p27_Piuri2023-10-03T16:01:58Z Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria Piuri, M. Hatfull, G.F. Antibiotics DST Fluoromycobacteriophage Mycobacteria Reporter phage Resistance carbenicillin cycloheximide ethambutol ethionamide isoniazid kanamycin rifampicin streptomycin tuberculostatic agent antibiotic sensitivity bacteriophage flow cytometry fluorescence microscopy fluoromycobacteriophage high throughput screening mycobacteriosis Mycobacterium Mycobacterium smegmatis infection nonhuman tuberculosis Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p27_Piuri |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Antibiotics DST Fluoromycobacteriophage Mycobacteria Reporter phage Resistance carbenicillin cycloheximide ethambutol ethionamide isoniazid kanamycin rifampicin streptomycin tuberculostatic agent antibiotic sensitivity bacteriophage flow cytometry fluorescence microscopy fluoromycobacteriophage high throughput screening mycobacteriosis Mycobacterium Mycobacterium smegmatis infection nonhuman tuberculosis |
spellingShingle |
Antibiotics DST Fluoromycobacteriophage Mycobacteria Reporter phage Resistance carbenicillin cycloheximide ethambutol ethionamide isoniazid kanamycin rifampicin streptomycin tuberculostatic agent antibiotic sensitivity bacteriophage flow cytometry fluorescence microscopy fluoromycobacteriophage high throughput screening mycobacteriosis Mycobacterium Mycobacterium smegmatis infection nonhuman tuberculosis Piuri, M. Hatfull, G.F. Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria |
topic_facet |
Antibiotics DST Fluoromycobacteriophage Mycobacteria Reporter phage Resistance carbenicillin cycloheximide ethambutol ethionamide isoniazid kanamycin rifampicin streptomycin tuberculostatic agent antibiotic sensitivity bacteriophage flow cytometry fluorescence microscopy fluoromycobacteriophage high throughput screening mycobacteriosis Mycobacterium Mycobacterium smegmatis infection nonhuman tuberculosis |
description |
Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. |
format |
SER |
author |
Piuri, M. Hatfull, G.F. |
author_facet |
Piuri, M. Hatfull, G.F. |
author_sort |
Piuri, M. |
title |
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria |
title_short |
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria |
title_full |
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria |
title_fullStr |
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria |
title_full_unstemmed |
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria |
title_sort |
fluoromycobacteriophages for drug susceptibility testing (dst) of mycobacteria |
url |
http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p27_Piuri |
work_keys_str_mv |
AT piurim fluoromycobacteriophagesfordrugsusceptibilitytestingdstofmycobacteria AT hatfullgf fluoromycobacteriophagesfordrugsusceptibilitytestingdstofmycobacteria |
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1782024502901735424 |