Methods for quantitative analysis of axonal cargo transport

Neurons rely on complex axonal transport mechanisms that mediate the intracellular dynamics of proteins, vesicles, and mitochondria along their high polarized structure. The fast improvement of live imaging techniques of fluorescent cargos allowed the identification of the diverse motion properties...

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Autores principales:	Alloatti, M., Bruno, L., Falzone, T.L.
Formato:	SER
Materias:	Anterograde Axonal transport Diffusive Pauses Retrograde Reversions Run lengths Segmental velocities animal cell animal cell culture DNA vector genetic transfection human human cell human cell culture imaging mouse nerve cell culture nerve fiber transport nonhuman pluripotent stem cell quantitative analysis rat animal axon biology cytology metabolism molecular imaging nerve cell procedures software Animals Axonal Transport Axons Computational Biology Humans Mice Molecular Imaging Neurons Rats Software
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10643745_v1727_n_p217_Alloatti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_10643745_v1727_n_p217_Alloatti
record_format	dspace
spelling	todo:paper_10643745_v1727_n_p217_Alloatti2023-10-03T16:01:57Z Methods for quantitative analysis of axonal cargo transport Alloatti, M. Bruno, L. Falzone, T.L. Anterograde Axonal transport Diffusive Pauses Retrograde Reversions Run lengths Segmental velocities animal cell animal cell culture DNA vector genetic transfection human human cell human cell culture imaging mouse nerve cell culture nerve fiber transport nonhuman pluripotent stem cell quantitative analysis rat animal axon biology cytology metabolism molecular imaging nerve cell nerve fiber transport procedures software Animals Axonal Transport Axons Computational Biology Humans Mice Molecular Imaging Neurons Rats Software Neurons rely on complex axonal transport mechanisms that mediate the intracellular dynamics of proteins, vesicles, and mitochondria along their high polarized structure. The fast improvement of live imaging techniques of fluorescent cargos allowed the identification of the diverse motion properties of different transported molecules. These properties arise as the result of molecular interactions between many players involved in axonal transport. Motor proteins, microtubule tracks, cargo association, and even axonal viscosity contribute to the proper axonal dynamics of different cargos. The unique properties in each cargo determine their distribution and location that is relevant to ensure neuronal cell activity and survival. This chapter provides a computational-based method for the generation of cargo trajectories and the identification of different motion regimes while cargo moves along axons. Then, the procedure to extract relevant parameters from active, diffusive, and confined motion is provided. These properties will allow a better comprehension of the nature and characteristics of cargo motion in living cells, therefore contributing to understanding the consequences of transport defects that arise during diseases of the nervous system. © Springer Science+Business Media, LLC 2018. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10643745_v1727_n_p217_Alloatti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Anterograde Axonal transport Diffusive Pauses Retrograde Reversions Run lengths Segmental velocities animal cell animal cell culture DNA vector genetic transfection human human cell human cell culture imaging mouse nerve cell culture nerve fiber transport nonhuman pluripotent stem cell quantitative analysis rat animal axon biology cytology metabolism molecular imaging nerve cell nerve fiber transport procedures software Animals Axonal Transport Axons Computational Biology Humans Mice Molecular Imaging Neurons Rats Software
spellingShingle	Anterograde Axonal transport Diffusive Pauses Retrograde Reversions Run lengths Segmental velocities animal cell animal cell culture DNA vector genetic transfection human human cell human cell culture imaging mouse nerve cell culture nerve fiber transport nonhuman pluripotent stem cell quantitative analysis rat animal axon biology cytology metabolism molecular imaging nerve cell nerve fiber transport procedures software Animals Axonal Transport Axons Computational Biology Humans Mice Molecular Imaging Neurons Rats Software Alloatti, M. Bruno, L. Falzone, T.L. Methods for quantitative analysis of axonal cargo transport
topic_facet	Anterograde Axonal transport Diffusive Pauses Retrograde Reversions Run lengths Segmental velocities animal cell animal cell culture DNA vector genetic transfection human human cell human cell culture imaging mouse nerve cell culture nerve fiber transport nonhuman pluripotent stem cell quantitative analysis rat animal axon biology cytology metabolism molecular imaging nerve cell nerve fiber transport procedures software Animals Axonal Transport Axons Computational Biology Humans Mice Molecular Imaging Neurons Rats Software
description	Neurons rely on complex axonal transport mechanisms that mediate the intracellular dynamics of proteins, vesicles, and mitochondria along their high polarized structure. The fast improvement of live imaging techniques of fluorescent cargos allowed the identification of the diverse motion properties of different transported molecules. These properties arise as the result of molecular interactions between many players involved in axonal transport. Motor proteins, microtubule tracks, cargo association, and even axonal viscosity contribute to the proper axonal dynamics of different cargos. The unique properties in each cargo determine their distribution and location that is relevant to ensure neuronal cell activity and survival. This chapter provides a computational-based method for the generation of cargo trajectories and the identification of different motion regimes while cargo moves along axons. Then, the procedure to extract relevant parameters from active, diffusive, and confined motion is provided. These properties will allow a better comprehension of the nature and characteristics of cargo motion in living cells, therefore contributing to understanding the consequences of transport defects that arise during diseases of the nervous system. © Springer Science+Business Media, LLC 2018.
format	SER
author	Alloatti, M. Bruno, L. Falzone, T.L.
author_facet	Alloatti, M. Bruno, L. Falzone, T.L.
author_sort	Alloatti, M.
title	Methods for quantitative analysis of axonal cargo transport
title_short	Methods for quantitative analysis of axonal cargo transport
title_full	Methods for quantitative analysis of axonal cargo transport
title_fullStr	Methods for quantitative analysis of axonal cargo transport
title_full_unstemmed	Methods for quantitative analysis of axonal cargo transport
title_sort	methods for quantitative analysis of axonal cargo transport
url	http://hdl.handle.net/20.500.12110/paper_10643745_v1727_n_p217_Alloatti
work_keys_str_mv	AT alloattim methodsforquantitativeanalysisofaxonalcargotransport AT brunol methodsforquantitativeanalysisofaxonalcargotransport AT falzonetl methodsforquantitativeanalysisofaxonalcargotransport
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Methods for quantitative analysis of axonal cargo transport

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