Improved ROS measurement in root hair cells
Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress respon...
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todo:paper_10643745_v1242_n_p67_Juarez2023-10-03T16:01:54Z Improved ROS measurement in root hair cells Juárez, S.P.D. Mangano, S. Estevez, J.M. 2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241–1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2′,7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p67_Juarez |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species |
| spellingShingle |
2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species Juárez, S.P.D. Mangano, S. Estevez, J.M. Improved ROS measurement in root hair cells |
| topic_facet |
2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species |
| description |
Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241–1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2′,7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015. |
| format |
SER |
| author |
Juárez, S.P.D. Mangano, S. Estevez, J.M. |
| author_facet |
Juárez, S.P.D. Mangano, S. Estevez, J.M. |
| author_sort |
Juárez, S.P.D. |
| title |
Improved ROS measurement in root hair cells |
| title_short |
Improved ROS measurement in root hair cells |
| title_full |
Improved ROS measurement in root hair cells |
| title_fullStr |
Improved ROS measurement in root hair cells |
| title_full_unstemmed |
Improved ROS measurement in root hair cells |
| title_sort |
improved ros measurement in root hair cells |
| url |
http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p67_Juarez |
| work_keys_str_mv |
AT juarezspd improvedrosmeasurementinroothaircells AT manganos improvedrosmeasurementinroothaircells AT estevezjm improvedrosmeasurementinroothaircells |
| _version_ |
1782030994010800128 |