Single photon fluorescent microlithography for live-cell imaging

Using fluorescent dyes to trigger the polymerization of a commercial polyurethane resin allows a rapid fabrication of micrometer and submicrometer sized fluorescent structures by one-photon absorption. Here, we show that standard He-Ne lasers emitting at 632.8 nm can be used to start the photopolyme...

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Detalles Bibliográficos
Autores principales: Kunik, D., Aramendia, P.F., Martínez, O.E.
Formato: JOUR
Materias:
Colocalization
Lithography
Microfluidics
Microscopy
Photopolymerization
Animals
Cells, Cultured
Laser Therapy, Low-Level
Melanophores
Microscopy, Confocal
Microscopy, Fluorescence
Polyurethanes
Urethane
Xenopus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1059910X_v73_n1_p20_Kunik
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_1059910X_v73_n1_p20_Kunik
record_format dspace
spelling todo:paper_1059910X_v73_n1_p20_Kunik2023-10-03T16:01:05Z Single photon fluorescent microlithography for live-cell imaging Kunik, D. Aramendia, P.F. Martínez, O.E. Colocalization Lithography Microfluidics Microscopy Photopolymerization Animals Cells, Cultured Laser Therapy, Low-Level Melanophores Microscopy, Confocal Microscopy, Fluorescence Polyurethanes Urethane Xenopus Using fluorescent dyes to trigger the polymerization of a commercial polyurethane resin allows a rapid fabrication of micrometer and submicrometer sized fluorescent structures by one-photon absorption. Here, we show that standard He-Ne lasers emitting at 632.8 nm can be used to start the photopolymerization and that very low laser power is required. This procedure allows the fabrication of fiduciary fluorescent references on standard glass coverslips, mica sheets, or gold-coated coverslips for laser scanning or standard fluorescent microscopy. The biocompatibility of the polymerized resin with cells in culture was tested by growing Xenopus melanophores and a standard laser scanning microscope was used to demonstrate that it is possible to use equipment readily available in several laboratories. We show that fluorescent structure with less than 10 nm in height may be used as references in fluorescence microscopy allowing a smooth environment for cell growth. Different dyes were tested and the conditions for one-photon polymerization were outlined. © 2009 Wiley-Liss, Inc. Fil:Kunik, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aramendia, P.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1059910X_v73_n1_p20_Kunik
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Colocalization
Lithography
Microfluidics
Microscopy
Photopolymerization
Animals
Cells, Cultured
Laser Therapy, Low-Level
Melanophores
Microscopy, Confocal
Microscopy, Fluorescence
Polyurethanes
Urethane
Xenopus
spellingShingle Colocalization
Lithography
Microfluidics
Microscopy
Photopolymerization
Animals
Cells, Cultured
Laser Therapy, Low-Level
Melanophores
Microscopy, Confocal
Microscopy, Fluorescence
Polyurethanes
Urethane
Xenopus
Kunik, D.
Aramendia, P.F.
Martínez, O.E.
Single photon fluorescent microlithography for live-cell imaging
topic_facet Colocalization
Lithography
Microfluidics
Microscopy
Photopolymerization
Animals
Cells, Cultured
Laser Therapy, Low-Level
Melanophores
Microscopy, Confocal
Microscopy, Fluorescence
Polyurethanes
Urethane
Xenopus
description Using fluorescent dyes to trigger the polymerization of a commercial polyurethane resin allows a rapid fabrication of micrometer and submicrometer sized fluorescent structures by one-photon absorption. Here, we show that standard He-Ne lasers emitting at 632.8 nm can be used to start the photopolymerization and that very low laser power is required. This procedure allows the fabrication of fiduciary fluorescent references on standard glass coverslips, mica sheets, or gold-coated coverslips for laser scanning or standard fluorescent microscopy. The biocompatibility of the polymerized resin with cells in culture was tested by growing Xenopus melanophores and a standard laser scanning microscope was used to demonstrate that it is possible to use equipment readily available in several laboratories. We show that fluorescent structure with less than 10 nm in height may be used as references in fluorescence microscopy allowing a smooth environment for cell growth. Different dyes were tested and the conditions for one-photon polymerization were outlined. © 2009 Wiley-Liss, Inc.
format JOUR
author Kunik, D.
Aramendia, P.F.
Martínez, O.E.
author_facet Kunik, D.
Aramendia, P.F.
Martínez, O.E.
author_sort Kunik, D.
title Single photon fluorescent microlithography for live-cell imaging
title_short Single photon fluorescent microlithography for live-cell imaging
title_full Single photon fluorescent microlithography for live-cell imaging
title_fullStr Single photon fluorescent microlithography for live-cell imaging
title_full_unstemmed Single photon fluorescent microlithography for live-cell imaging
title_sort single photon fluorescent microlithography for live-cell imaging
url http://hdl.handle.net/20.500.12110/paper_1059910X_v73_n1_p20_Kunik
work_keys_str_mv AT kunikd singlephotonfluorescentmicrolithographyforlivecellimaging
AT aramendiapf singlephotonfluorescentmicrolithographyforlivecellimaging
AT martinezoe singlephotonfluorescentmicrolithographyforlivecellimaging
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