Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET)...

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Autores principales:	Grecco, H.E., Lidke, K.A., Heintzmann, R., Lidke, D.S., Spagnuolo, C., Martinez, O.E., Jares-Erijman, E.A., Jovin, T.M.
Formato:	JOUR
Materias:	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1059910X_v65_n4-5_p169_Grecco
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1059910X_v65_n4-5_p169_Grecco
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spelling	todo:paper_1059910X_v65_n4-5_p169_Grecco2023-10-03T16:01:04Z Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells Grecco, H.E. Lidke, K.A. Heintzmann, R. Lidke, D.S. Spagnuolo, C. Martinez, O.E. Jares-Erijman, E.A. Jovin, T.M. Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). © 2005 Wiley-Liss, Inc. Fil:Grecco, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spagnuolo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martinez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1059910X_v65_n4-5_p169_Grecco
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin
spellingShingle	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin Grecco, H.E. Lidke, K.A. Heintzmann, R. Lidke, D.S. Spagnuolo, C. Martinez, O.E. Jares-Erijman, E.A. Jovin, T.M. Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
topic_facet	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin
description	In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). © 2005 Wiley-Liss, Inc.
format	JOUR
author	Grecco, H.E. Lidke, K.A. Heintzmann, R. Lidke, D.S. Spagnuolo, C. Martinez, O.E. Jares-Erijman, E.A. Jovin, T.M.
author_facet	Grecco, H.E. Lidke, K.A. Heintzmann, R. Lidke, D.S. Spagnuolo, C. Martinez, O.E. Jares-Erijman, E.A. Jovin, T.M.
author_sort	Grecco, H.E.
title	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_short	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_full	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_fullStr	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_full_unstemmed	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_sort	ensemble and single particle photophysical properties (two-photon excitation, anisotropy, fret, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
url	http://hdl.handle.net/20.500.12110/paper_1059910X_v65_n4-5_p169_Grecco
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Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

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