VEGF and CD31 association in pituitary adenomas
Pituitary tumors are usually less vascularized than the normal pituitary, and the role of angiogenesis in these adenomas is contentious. Appraisal of microvascular density and expression of the potent angiogenic vascular endothelial growth factor (VEGF) by immunohistochemistry has yielded controvers...
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todo:paper_10463976_v21_n3_p154_Cristina2023-10-03T15:58:27Z VEGF and CD31 association in pituitary adenomas Cristina, C. Perez-Millan, M.I. Luque, G. Dulce, R.A. Sevlever, G. Berner, S.I. Becu-Villalobos, D. Angiogenesis CD31 Pelioisis Pituitary adenoma Proliferation VEGF CD31 antigen cycline Ki 67 antigen vasculotropin adult age angiogenesis article carcinogenesis cell proliferation correlation analysis disease course epithelium cell female gender growth hormone secreting adenoma histopathology human human tissue hypophysis adenoma major clinical study male mutation priority journal prolactinoma protein expression tumor growth Western blotting Adenoma Adult Antigens, CD31 Blotting, Western Female Humans Male Pituitary Neoplasms Tumor Markers, Biological Vascular Endothelial Growth Factor A Pituitary tumors are usually less vascularized than the normal pituitary, and the role of angiogenesis in these adenomas is contentious. Appraisal of microvascular density and expression of the potent angiogenic vascular endothelial growth factor (VEGF) by immunohistochemistry has yielded controversial results, as a broad spectrum of immunostaining can be found. We determined the protein expression of VEGF and CD31, an endothelial marker, in a series of 56 surgically removed pituitary adenomas using Western blot assay. Prolactinomas had higher VEGF protein expression compared to nonfunctioning or ACTH- and GH-secreting adenomas, while CD31 was similar in the different adenoma histotypes. VEGF and CD31 were not affected by sex, age, years of adenoma evolution, or proliferation rate (Ki67 and PCNA) for all adenoma types. Only in nonfunctioning adenomas CD31 concentration increased significantly with age. There was a positive correlation between CD31 and VEGF expression when all adenoma histotypes were considered, or when prolactinomas and nonfunctioning adenomas were evaluated separately. The positive association of VEGF and CD31 expression suggests the participation of angiogenesis in adenoma development, while epithelial cell proliferation in pituitary tumors is not directly related to VEGF or CD31 expression, and other factors, such as primary genetic alterations may be involved. © 2010 Springer Science+Business Media, LLC. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10463976_v21_n3_p154_Cristina |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Angiogenesis CD31 Pelioisis Pituitary adenoma Proliferation VEGF CD31 antigen cycline Ki 67 antigen vasculotropin adult age angiogenesis article carcinogenesis cell proliferation correlation analysis disease course epithelium cell female gender growth hormone secreting adenoma histopathology human human tissue hypophysis adenoma major clinical study male mutation priority journal prolactinoma protein expression tumor growth Western blotting Adenoma Adult Antigens, CD31 Blotting, Western Female Humans Male Pituitary Neoplasms Tumor Markers, Biological Vascular Endothelial Growth Factor A |
spellingShingle |
Angiogenesis CD31 Pelioisis Pituitary adenoma Proliferation VEGF CD31 antigen cycline Ki 67 antigen vasculotropin adult age angiogenesis article carcinogenesis cell proliferation correlation analysis disease course epithelium cell female gender growth hormone secreting adenoma histopathology human human tissue hypophysis adenoma major clinical study male mutation priority journal prolactinoma protein expression tumor growth Western blotting Adenoma Adult Antigens, CD31 Blotting, Western Female Humans Male Pituitary Neoplasms Tumor Markers, Biological Vascular Endothelial Growth Factor A Cristina, C. Perez-Millan, M.I. Luque, G. Dulce, R.A. Sevlever, G. Berner, S.I. Becu-Villalobos, D. VEGF and CD31 association in pituitary adenomas |
topic_facet |
Angiogenesis CD31 Pelioisis Pituitary adenoma Proliferation VEGF CD31 antigen cycline Ki 67 antigen vasculotropin adult age angiogenesis article carcinogenesis cell proliferation correlation analysis disease course epithelium cell female gender growth hormone secreting adenoma histopathology human human tissue hypophysis adenoma major clinical study male mutation priority journal prolactinoma protein expression tumor growth Western blotting Adenoma Adult Antigens, CD31 Blotting, Western Female Humans Male Pituitary Neoplasms Tumor Markers, Biological Vascular Endothelial Growth Factor A |
description |
Pituitary tumors are usually less vascularized than the normal pituitary, and the role of angiogenesis in these adenomas is contentious. Appraisal of microvascular density and expression of the potent angiogenic vascular endothelial growth factor (VEGF) by immunohistochemistry has yielded controversial results, as a broad spectrum of immunostaining can be found. We determined the protein expression of VEGF and CD31, an endothelial marker, in a series of 56 surgically removed pituitary adenomas using Western blot assay. Prolactinomas had higher VEGF protein expression compared to nonfunctioning or ACTH- and GH-secreting adenomas, while CD31 was similar in the different adenoma histotypes. VEGF and CD31 were not affected by sex, age, years of adenoma evolution, or proliferation rate (Ki67 and PCNA) for all adenoma types. Only in nonfunctioning adenomas CD31 concentration increased significantly with age. There was a positive correlation between CD31 and VEGF expression when all adenoma histotypes were considered, or when prolactinomas and nonfunctioning adenomas were evaluated separately. The positive association of VEGF and CD31 expression suggests the participation of angiogenesis in adenoma development, while epithelial cell proliferation in pituitary tumors is not directly related to VEGF or CD31 expression, and other factors, such as primary genetic alterations may be involved. © 2010 Springer Science+Business Media, LLC. |
format |
JOUR |
author |
Cristina, C. Perez-Millan, M.I. Luque, G. Dulce, R.A. Sevlever, G. Berner, S.I. Becu-Villalobos, D. |
author_facet |
Cristina, C. Perez-Millan, M.I. Luque, G. Dulce, R.A. Sevlever, G. Berner, S.I. Becu-Villalobos, D. |
author_sort |
Cristina, C. |
title |
VEGF and CD31 association in pituitary adenomas |
title_short |
VEGF and CD31 association in pituitary adenomas |
title_full |
VEGF and CD31 association in pituitary adenomas |
title_fullStr |
VEGF and CD31 association in pituitary adenomas |
title_full_unstemmed |
VEGF and CD31 association in pituitary adenomas |
title_sort |
vegf and cd31 association in pituitary adenomas |
url |
http://hdl.handle.net/20.500.12110/paper_10463976_v21_n3_p154_Cristina |
work_keys_str_mv |
AT cristinac vegfandcd31associationinpituitaryadenomas AT perezmillanmi vegfandcd31associationinpituitaryadenomas AT luqueg vegfandcd31associationinpituitaryadenomas AT dulcera vegfandcd31associationinpituitaryadenomas AT sevleverg vegfandcd31associationinpituitaryadenomas AT bernersi vegfandcd31associationinpituitaryadenomas AT becuvillalobosd vegfandcd31associationinpituitaryadenomas |
_version_ |
1782028437691564032 |