Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele

Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased...

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Autores principales:	Buffone, M.G., Brugo-Olmedo, S., Calamera, J.C., Verstraeten, S.V., Urrutia, F., Grippo, L., Corbetta, J.P., Doncel, G.F.
Formato:	JOUR
Materias:	Capacitation Human spermatozoa Hyperactivation Membrane properties Semen pathology Signal transduction protein tyrosine kinase article cell membrane fluidity clinical article computer analysis enzyme phosphorylation human human cell immunofluorescence incidence male male infertility pathophysiology priority journal semen analysis spermatozoon spermatozoon capacitation spermatozoon motility varicocele Western blotting Adult Cell Membrane Humans Infertility, Male Male Membrane Fluidity Phosphorylation Protein-Tyrosine Kinases Sperm Motility Spermatozoa Varicocele
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1040452X_v73_n12_p1591_Buffone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_1040452X_v73_n12_p1591_Buffone2023-10-03T15:57:51Z Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele Buffone, M.G. Brugo-Olmedo, S. Calamera, J.C. Verstraeten, S.V. Urrutia, F. Grippo, L. Corbetta, J.P. Doncel, G.F. Capacitation Human spermatozoa Hyperactivation Membrane properties Semen pathology Signal transduction protein tyrosine kinase article cell membrane fluidity clinical article computer analysis enzyme phosphorylation human human cell immunofluorescence incidence male male infertility pathophysiology priority journal semen analysis spermatozoon spermatozoon capacitation spermatozoon motility varicocele Western blotting Adult Cell Membrane Humans Infertility, Male Male Membrane Fluidity Phosphorylation Protein-Tyrosine Kinases Sperm Motility Spermatozoa Varicocele Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity and protein tyrosine phosphorylation. Given the reported association between varicocele, oxidative stress, and sperm dysfunction, we hypothesized that spermatozoa from infertile patients with varicocele might have a combined defect at the level of membrane fluidity and protein tyrosine phosphorylation. Semen samples from infertile patients with and without grade II/III left varicocele were evaluated for motion parameters (computer-assisted semen analysis [CASA]), hyperactivation (CASA), incidence and intensity of protein tyrosine phosphorylation (phosphotyrosine immunofluorescence and western blotting), and membrane fluidity (Laurdan fluorometry), before and after a capacitating incubation (6 hr at 37°C in Ham's F10/BSA, 5% CO2). Spermatozoa from varicocele samples presented a decreased response to the capacitating challenge, showing significantly lower motility, hyperactivation, incidence and intensity of tyrosine phosphorylation, and membrane fluidity. The findings reported in this article indicate that the sperm dysfunction associated to infertile varicocele coexists with decreased sperm plasma membrane fluidity and tyrosine phosphorylation. These deficiencies represent potential new pathophysiological mechanisms underlying varicocele-related infertility. © 2006 Wiley-Liss, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1040452X_v73_n12_p1591_Buffone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Capacitation Human spermatozoa Hyperactivation Membrane properties Semen pathology Signal transduction protein tyrosine kinase article cell membrane fluidity clinical article computer analysis enzyme phosphorylation human human cell immunofluorescence incidence male male infertility pathophysiology priority journal semen analysis spermatozoon spermatozoon capacitation spermatozoon motility varicocele Western blotting Adult Cell Membrane Humans Infertility, Male Male Membrane Fluidity Phosphorylation Protein-Tyrosine Kinases Sperm Motility Spermatozoa Varicocele
spellingShingle	Capacitation Human spermatozoa Hyperactivation Membrane properties Semen pathology Signal transduction protein tyrosine kinase article cell membrane fluidity clinical article computer analysis enzyme phosphorylation human human cell immunofluorescence incidence male male infertility pathophysiology priority journal semen analysis spermatozoon spermatozoon capacitation spermatozoon motility varicocele Western blotting Adult Cell Membrane Humans Infertility, Male Male Membrane Fluidity Phosphorylation Protein-Tyrosine Kinases Sperm Motility Spermatozoa Varicocele Buffone, M.G. Brugo-Olmedo, S. Calamera, J.C. Verstraeten, S.V. Urrutia, F. Grippo, L. Corbetta, J.P. Doncel, G.F. Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
topic_facet	Capacitation Human spermatozoa Hyperactivation Membrane properties Semen pathology Signal transduction protein tyrosine kinase article cell membrane fluidity clinical article computer analysis enzyme phosphorylation human human cell immunofluorescence incidence male male infertility pathophysiology priority journal semen analysis spermatozoon spermatozoon capacitation spermatozoon motility varicocele Western blotting Adult Cell Membrane Humans Infertility, Male Male Membrane Fluidity Phosphorylation Protein-Tyrosine Kinases Sperm Motility Spermatozoa Varicocele
description	Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity and protein tyrosine phosphorylation. Given the reported association between varicocele, oxidative stress, and sperm dysfunction, we hypothesized that spermatozoa from infertile patients with varicocele might have a combined defect at the level of membrane fluidity and protein tyrosine phosphorylation. Semen samples from infertile patients with and without grade II/III left varicocele were evaluated for motion parameters (computer-assisted semen analysis [CASA]), hyperactivation (CASA), incidence and intensity of protein tyrosine phosphorylation (phosphotyrosine immunofluorescence and western blotting), and membrane fluidity (Laurdan fluorometry), before and after a capacitating incubation (6 hr at 37°C in Ham's F10/BSA, 5% CO2). Spermatozoa from varicocele samples presented a decreased response to the capacitating challenge, showing significantly lower motility, hyperactivation, incidence and intensity of tyrosine phosphorylation, and membrane fluidity. The findings reported in this article indicate that the sperm dysfunction associated to infertile varicocele coexists with decreased sperm plasma membrane fluidity and tyrosine phosphorylation. These deficiencies represent potential new pathophysiological mechanisms underlying varicocele-related infertility. © 2006 Wiley-Liss, Inc.
format	JOUR
author	Buffone, M.G. Brugo-Olmedo, S. Calamera, J.C. Verstraeten, S.V. Urrutia, F. Grippo, L. Corbetta, J.P. Doncel, G.F.
author_facet	Buffone, M.G. Brugo-Olmedo, S. Calamera, J.C. Verstraeten, S.V. Urrutia, F. Grippo, L. Corbetta, J.P. Doncel, G.F.
author_sort	Buffone, M.G.
title	Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
title_short	Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
title_full	Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
title_fullStr	Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
title_full_unstemmed	Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
title_sort	decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
url	http://hdl.handle.net/20.500.12110/paper_1040452X_v73_n12_p1591_Buffone
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Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele

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