In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation
Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobut...
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todo:paper_1040452X_v67_n4_p478_Ritta2023-10-03T15:57:50Z In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation Ritta, M.N. Bas, D.E. Tartaglione, C.M. Bovine Capacitation Chlortetracycline GABA Spermatozoa 4 aminobutyric acid arecaidine bicuculline cyclic AMP muscimol picrotoxin animal cell article binding site calcium cell level cation transport cattle cell suspension controlled study fertilization in vitro study membrane binding nonhuman priority journal radioreceptor assay Scatchard plot sperm spermatozoon capacitation spermatozoon maturation Animals Calcium Cattle Cyclic AMP GABA Agonists gamma-Aminobutyric Acid Male Protein Binding Receptors, GABA-A Sperm Capacitation Spermatozoa Bovinae Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobutyric acid (GABA) has recently become as a putative modulator of sperm function. The aim of this study was to explore the presence of a GABAergic regulation of bovine sperm capacitation as well as the possible intracellular mechanisms involved. GABA was detected in fresh semen by a sensitive radioreceptor assay (spermatozoa, 0.064 ± 0.003 nmoles/106 cells; seminal plasma, 23.21 ± 1.16 nmoles/ml). Scatchard analysis of [3 H]-muscimol binding to sperm membranes yielded a linear plot consistent with a single population of binding sites (Kd = 3.87 nM, Bmax = 417 fmol/mg prot.). [3H]-muscimol specific binding to sperm membranes was significantly inhibited by the GABA A receptor (GABA A-R) antagonist bicuculline and by the agonists muscimol and isoguvacine. Addition of GABA to the incubation medium resulted in a concentration-dependent increase in the percentage of capacitated spermatozoa (chlortetracycline assay). We observed a significant increment on intracellular calcium and cyclic 3′,5′ adenosine monophosphate (cAMP) concentrations induced by GABA, being the cation influx abolished when the cell suspensions were coincubated with the antagonists bicuculline or picrotoxin. It is concluded that GABA induces sperm capacitation through an intracellular mechanism dependent on calcium influx and cAMP accumulation mediated by a specific GABA A-R. © 2004 Wiley-Liss, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1040452X_v67_n4_p478_Ritta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bovine Capacitation Chlortetracycline GABA Spermatozoa 4 aminobutyric acid arecaidine bicuculline cyclic AMP muscimol picrotoxin animal cell article binding site calcium cell level cation transport cattle cell suspension controlled study fertilization in vitro study membrane binding nonhuman priority journal radioreceptor assay Scatchard plot sperm spermatozoon capacitation spermatozoon maturation Animals Calcium Cattle Cyclic AMP GABA Agonists gamma-Aminobutyric Acid Male Protein Binding Receptors, GABA-A Sperm Capacitation Spermatozoa Bovinae |
| spellingShingle |
Bovine Capacitation Chlortetracycline GABA Spermatozoa 4 aminobutyric acid arecaidine bicuculline cyclic AMP muscimol picrotoxin animal cell article binding site calcium cell level cation transport cattle cell suspension controlled study fertilization in vitro study membrane binding nonhuman priority journal radioreceptor assay Scatchard plot sperm spermatozoon capacitation spermatozoon maturation Animals Calcium Cattle Cyclic AMP GABA Agonists gamma-Aminobutyric Acid Male Protein Binding Receptors, GABA-A Sperm Capacitation Spermatozoa Bovinae Ritta, M.N. Bas, D.E. Tartaglione, C.M. In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation |
| topic_facet |
Bovine Capacitation Chlortetracycline GABA Spermatozoa 4 aminobutyric acid arecaidine bicuculline cyclic AMP muscimol picrotoxin animal cell article binding site calcium cell level cation transport cattle cell suspension controlled study fertilization in vitro study membrane binding nonhuman priority journal radioreceptor assay Scatchard plot sperm spermatozoon capacitation spermatozoon maturation Animals Calcium Cattle Cyclic AMP GABA Agonists gamma-Aminobutyric Acid Male Protein Binding Receptors, GABA-A Sperm Capacitation Spermatozoa Bovinae |
| description |
Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobutyric acid (GABA) has recently become as a putative modulator of sperm function. The aim of this study was to explore the presence of a GABAergic regulation of bovine sperm capacitation as well as the possible intracellular mechanisms involved. GABA was detected in fresh semen by a sensitive radioreceptor assay (spermatozoa, 0.064 ± 0.003 nmoles/106 cells; seminal plasma, 23.21 ± 1.16 nmoles/ml). Scatchard analysis of [3 H]-muscimol binding to sperm membranes yielded a linear plot consistent with a single population of binding sites (Kd = 3.87 nM, Bmax = 417 fmol/mg prot.). [3H]-muscimol specific binding to sperm membranes was significantly inhibited by the GABA A receptor (GABA A-R) antagonist bicuculline and by the agonists muscimol and isoguvacine. Addition of GABA to the incubation medium resulted in a concentration-dependent increase in the percentage of capacitated spermatozoa (chlortetracycline assay). We observed a significant increment on intracellular calcium and cyclic 3′,5′ adenosine monophosphate (cAMP) concentrations induced by GABA, being the cation influx abolished when the cell suspensions were coincubated with the antagonists bicuculline or picrotoxin. It is concluded that GABA induces sperm capacitation through an intracellular mechanism dependent on calcium influx and cAMP accumulation mediated by a specific GABA A-R. © 2004 Wiley-Liss, Inc. |
| format |
JOUR |
| author |
Ritta, M.N. Bas, D.E. Tartaglione, C.M. |
| author_facet |
Ritta, M.N. Bas, D.E. Tartaglione, C.M. |
| author_sort |
Ritta, M.N. |
| title |
In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation |
| title_short |
In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation |
| title_full |
In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation |
| title_fullStr |
In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation |
| title_full_unstemmed |
In Vitro Effect of Gamma-Aminobutyric Acid on Bovine Spermatozoa Capacitation |
| title_sort |
in vitro effect of gamma-aminobutyric acid on bovine spermatozoa capacitation |
| url |
http://hdl.handle.net/20.500.12110/paper_1040452X_v67_n4_p478_Ritta |
| work_keys_str_mv |
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| _version_ |
1782025276427862016 |