Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controve...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_1040452X_v39_n3_p280_Saragueta |
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todo:paper_1040452X_v39_n3_p280_Saragueta2023-10-03T15:57:46Z Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm Saragüeta, P. Lanuza, G. V. Miranda, P. G. Tezón, J. Lino Baraño, J. Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controversial. We performed a three step purification scheme for hFF and all the fractions were screened for the AR‐inducing activity. AR activity was associated with a protein fraction of Mr > 180 kD that on further analysis under PAGE was found to be composed by subunits of apparent Mr 50,000 and 29,000. The N‐terminal sequences of these bands showed a 100% homology with the heavy and light chains of human lgG. A polyclonal antibody raised against the purified protein and anti‐human lgG were both able to suppress the acrosome reactioninducing activity of crude hFF. However, neither normal human serum nor a purified preparation of human lgG were able to mimic the AR‐inducing activity of hFF. We concluded that the AR‐inducing activity of hFF is, at least in part, due to the presence of antisperm antibodies. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc. Fil:Saragüeta, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1040452X_v39_n3_p280_Saragueta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't |
| spellingShingle |
Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't Saragüeta, P. Lanuza, G. V. Miranda, P. G. Tezón, J. Lino Baraño, J. Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm |
| topic_facet |
Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't |
| description |
The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controversial. We performed a three step purification scheme for hFF and all the fractions were screened for the AR‐inducing activity. AR activity was associated with a protein fraction of Mr > 180 kD that on further analysis under PAGE was found to be composed by subunits of apparent Mr 50,000 and 29,000. The N‐terminal sequences of these bands showed a 100% homology with the heavy and light chains of human lgG. A polyclonal antibody raised against the purified protein and anti‐human lgG were both able to suppress the acrosome reactioninducing activity of crude hFF. However, neither normal human serum nor a purified preparation of human lgG were able to mimic the AR‐inducing activity of hFF. We concluded that the AR‐inducing activity of hFF is, at least in part, due to the presence of antisperm antibodies. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc. |
| format |
JOUR |
| author |
Saragüeta, P. Lanuza, G. V. Miranda, P. G. Tezón, J. Lino Baraño, J. |
| author_facet |
Saragüeta, P. Lanuza, G. V. Miranda, P. G. Tezón, J. Lino Baraño, J. |
| author_sort |
Saragüeta, P. |
| title |
Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm |
| title_short |
Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm |
| title_full |
Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm |
| title_fullStr |
Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm |
| title_full_unstemmed |
Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm |
| title_sort |
immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm |
| url |
http://hdl.handle.net/20.500.12110/paper_1040452X_v39_n3_p280_Saragueta |
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1807317286903611392 |