Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation
Interleukin-l (IL-1) is an important mediator in the mechanisms underlying the immune and inflammatory responses. It has pleiotropic effects in host defense and, when present in high concentrations, participates in the development of pathological processes. IL-1 is the most potent cytokine in the ac...
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todo:paper_10217401_v3_n4_p227_Pereda2023-10-03T15:56:40Z Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation Pereda, M.P. Castro, C.P. Costas, M. Nahmod, V.E. Stalla, G.K. Holsboer, F. Arzt, E. Endotoxin Glucocorticoids Interleukin-l Monocytes dexamethasone glucocorticoid hydrocortisone interleukin 1 receptor blocking agent interleukin 1beta lipopolysaccharide messenger rna mifepristone adult article human human cell monocyte normal human priority journal Cells, Cultured Dose-Response Relationship, Drug Endotoxins Glucocorticoids Homeostasis Humans Interleukin-1 Lipopolysaccharides Monocytes Interleukin-l (IL-1) is an important mediator in the mechanisms underlying the immune and inflammatory responses. It has pleiotropic effects in host defense and, when present in high concentrations, participates in the development of pathological processes. IL-1 is the most potent cytokine in the activation of the hypothalamic-pituitary-adrenal axis during infection and therefore leads to a glucocorticoid increase. Glucocorticoids in a feedback loop inhibit the production of IL-1 induced by endotoxin. IL-1 also induces its own synthesis. In this report, we examine the role of glucocorticoids in the regulation of IL-1 autoregulatory induction in human monocytes at the level of IL-1 protein production and mRNA accumulation. Using recombinant IL1 receptor antagonist we established that endogenously produced IL-1 affects induction of IL-1βprotein by lipopolysaccharide (LPS) at the level of mRNA expression. The inhibition of LPS-stimulated IL-1βproduction and mRNA expression by glucocorticoids (dexamethasone and Cortisol) reaches the same level with glucocorticoids alone or in combination with rIL-1ra. IL-1βmRNA induced by exogenously added IL-1βwas also inhibited by glucocorticoids. These results indicate that glucocorticoids inhibit the autoregulatory loop of IL-1 in LPS-stimulated monocytes and constitute a mechanism for controlling IL-1 feedback stimulation. © 1996 S. Karger AG, Basel. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castro, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Costas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10217401_v3_n4_p227_Pereda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Endotoxin Glucocorticoids Interleukin-l Monocytes dexamethasone glucocorticoid hydrocortisone interleukin 1 receptor blocking agent interleukin 1beta lipopolysaccharide messenger rna mifepristone adult article human human cell monocyte normal human priority journal Cells, Cultured Dose-Response Relationship, Drug Endotoxins Glucocorticoids Homeostasis Humans Interleukin-1 Lipopolysaccharides Monocytes |
| spellingShingle |
Endotoxin Glucocorticoids Interleukin-l Monocytes dexamethasone glucocorticoid hydrocortisone interleukin 1 receptor blocking agent interleukin 1beta lipopolysaccharide messenger rna mifepristone adult article human human cell monocyte normal human priority journal Cells, Cultured Dose-Response Relationship, Drug Endotoxins Glucocorticoids Homeostasis Humans Interleukin-1 Lipopolysaccharides Monocytes Pereda, M.P. Castro, C.P. Costas, M. Nahmod, V.E. Stalla, G.K. Holsboer, F. Arzt, E. Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation |
| topic_facet |
Endotoxin Glucocorticoids Interleukin-l Monocytes dexamethasone glucocorticoid hydrocortisone interleukin 1 receptor blocking agent interleukin 1beta lipopolysaccharide messenger rna mifepristone adult article human human cell monocyte normal human priority journal Cells, Cultured Dose-Response Relationship, Drug Endotoxins Glucocorticoids Homeostasis Humans Interleukin-1 Lipopolysaccharides Monocytes |
| description |
Interleukin-l (IL-1) is an important mediator in the mechanisms underlying the immune and inflammatory responses. It has pleiotropic effects in host defense and, when present in high concentrations, participates in the development of pathological processes. IL-1 is the most potent cytokine in the activation of the hypothalamic-pituitary-adrenal axis during infection and therefore leads to a glucocorticoid increase. Glucocorticoids in a feedback loop inhibit the production of IL-1 induced by endotoxin. IL-1 also induces its own synthesis. In this report, we examine the role of glucocorticoids in the regulation of IL-1 autoregulatory induction in human monocytes at the level of IL-1 protein production and mRNA accumulation. Using recombinant IL1 receptor antagonist we established that endogenously produced IL-1 affects induction of IL-1βprotein by lipopolysaccharide (LPS) at the level of mRNA expression. The inhibition of LPS-stimulated IL-1βproduction and mRNA expression by glucocorticoids (dexamethasone and Cortisol) reaches the same level with glucocorticoids alone or in combination with rIL-1ra. IL-1βmRNA induced by exogenously added IL-1βwas also inhibited by glucocorticoids. These results indicate that glucocorticoids inhibit the autoregulatory loop of IL-1 in LPS-stimulated monocytes and constitute a mechanism for controlling IL-1 feedback stimulation. © 1996 S. Karger AG, Basel. |
| format |
JOUR |
| author |
Pereda, M.P. Castro, C.P. Costas, M. Nahmod, V.E. Stalla, G.K. Holsboer, F. Arzt, E. |
| author_facet |
Pereda, M.P. Castro, C.P. Costas, M. Nahmod, V.E. Stalla, G.K. Holsboer, F. Arzt, E. |
| author_sort |
Pereda, M.P. |
| title |
Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation |
| title_short |
Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation |
| title_full |
Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation |
| title_fullStr |
Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation |
| title_full_unstemmed |
Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation |
| title_sort |
glucocorticoids inhibit the autoregulatory induction of lnterleukin-1 in monocytes after endotoxin stimulation |
| url |
http://hdl.handle.net/20.500.12110/paper_10217401_v3_n4_p227_Pereda |
| work_keys_str_mv |
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