Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation
Background/Aims: The maternal-fetal interface is a unique immunological site that generates an adequate microenvironment during pregnancy, recognizing and eliminating infections and tolerating the trophoblast/placenta unit. For that purpose, trophoblast cells display several tolerogenic mechanisms t...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10217401_v21_n1_p21_Fraccaroli |
| Aporte de: |
| id |
todo:paper_10217401_v21_n1_p21_Fraccaroli |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_10217401_v21_n1_p21_Fraccaroli2023-10-03T15:56:39Z Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation Fraccaroli, L. Grasso, E. Hauk, V. Cortelezzi, M. Pérez Leirós, C. Ramhorst, R. Early pregnancy Human implantation Tolerance and pregnancy Vasoactive intestinal peptide interleukin 10 interleukin 6 lipopolysaccharide monocyte chemotactic protein 1 nitrite transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 CD14 antigen CD19 antigen lipopolysaccharide vasoactive intestinal polypeptide article cell interaction coculture controlled study cytokine production female human human cell immunomodulation immunostimulation in vitro study leukocyte microenvironment mother peripheral blood mononuclear cell priority journal protein expression protein function reverse transcription polymerase chain reaction trophoblast upregulation Western blotting Article B lymphocyte CD4+ T lymphocyte cell line flow cytometry homeostasis immune homeostasis inflammation swan 71 cell line trophoblast trophoblast maternal leukocyte interaction CD4-Positive T-Lymphocytes Coculture Techniques Cytokines Dose-Response Relationship, Drug Female Flow Cytometry Gene Expression Regulation Homeostasis Humans Leukocytes Lipopolysaccharides Nitrites Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I Time Factors Transforming Growth Factor beta Trophoblasts Vasoactive Intestinal Peptide Background/Aims: The maternal-fetal interface is a unique immunological site that generates an adequate microenvironment during pregnancy, recognizing and eliminating infections and tolerating the trophoblast/placenta unit. For that purpose, trophoblast cells display several tolerogenic mechanisms to allow fetal survival, such as production of the neuropeptide vasoactive intestinal peptide (VIP). Here we investigated the contribution of VIP to maintain homeostasis at the maternal-placental interface under lipopolysaccharide (LPS) stimulation. Methods: We performed cocultures between trophoblast cells (Swan-71 cell line) and maternal leukocytes obtained from fertile women as an in vitro model of maternal-placental interaction, and we focused on the effects of LPS on the modulation of VIP and their receptors (VPAC1 and VPAC 2). Results: VIP could prevent the upregulation of IL-6, MCP-1, and nitrite production and maintain the production of IL-10 and TGF-β under LPS (10 μg/ml) stimulation after 48 h of coculture. To gain deeper insight into the mechanisms of how VIP could contribute to a tolerogenic microenvironment even in the presence of LPS, we investigated VIP production by maternal leukocytes and observed a significant increase in the frequency of CD4+VIP+ cells after interaction with Swan-71 cells in the presence of LPS. LPS increased VIP and inducible receptor VPAC2 expression directly on trophoblast cells in a dose- and time-dependent manner. Conclusions: The present results suggest that VIP might act as an additional homeostatic mechanism during early stages at the maternal-placental interface to control exacerbated inflammatory responses such as the ones observed in intrauterine infections. © 2013 S. Karger AG, Basel. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10217401_v21_n1_p21_Fraccaroli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Early pregnancy Human implantation Tolerance and pregnancy Vasoactive intestinal peptide interleukin 10 interleukin 6 lipopolysaccharide monocyte chemotactic protein 1 nitrite transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 CD14 antigen CD19 antigen lipopolysaccharide vasoactive intestinal polypeptide article cell interaction coculture controlled study cytokine production female human human cell immunomodulation immunostimulation in vitro study leukocyte microenvironment mother peripheral blood mononuclear cell priority journal protein expression protein function reverse transcription polymerase chain reaction trophoblast upregulation Western blotting Article B lymphocyte CD4+ T lymphocyte cell line flow cytometry homeostasis immune homeostasis inflammation swan 71 cell line trophoblast trophoblast maternal leukocyte interaction CD4-Positive T-Lymphocytes Coculture Techniques Cytokines Dose-Response Relationship, Drug Female Flow Cytometry Gene Expression Regulation Homeostasis Humans Leukocytes Lipopolysaccharides Nitrites Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I Time Factors Transforming Growth Factor beta Trophoblasts Vasoactive Intestinal Peptide |
| spellingShingle |
Early pregnancy Human implantation Tolerance and pregnancy Vasoactive intestinal peptide interleukin 10 interleukin 6 lipopolysaccharide monocyte chemotactic protein 1 nitrite transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 CD14 antigen CD19 antigen lipopolysaccharide vasoactive intestinal polypeptide article cell interaction coculture controlled study cytokine production female human human cell immunomodulation immunostimulation in vitro study leukocyte microenvironment mother peripheral blood mononuclear cell priority journal protein expression protein function reverse transcription polymerase chain reaction trophoblast upregulation Western blotting Article B lymphocyte CD4+ T lymphocyte cell line flow cytometry homeostasis immune homeostasis inflammation swan 71 cell line trophoblast trophoblast maternal leukocyte interaction CD4-Positive T-Lymphocytes Coculture Techniques Cytokines Dose-Response Relationship, Drug Female Flow Cytometry Gene Expression Regulation Homeostasis Humans Leukocytes Lipopolysaccharides Nitrites Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I Time Factors Transforming Growth Factor beta Trophoblasts Vasoactive Intestinal Peptide Fraccaroli, L. Grasso, E. Hauk, V. Cortelezzi, M. Pérez Leirós, C. Ramhorst, R. Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation |
| topic_facet |
Early pregnancy Human implantation Tolerance and pregnancy Vasoactive intestinal peptide interleukin 10 interleukin 6 lipopolysaccharide monocyte chemotactic protein 1 nitrite transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 CD14 antigen CD19 antigen lipopolysaccharide vasoactive intestinal polypeptide article cell interaction coculture controlled study cytokine production female human human cell immunomodulation immunostimulation in vitro study leukocyte microenvironment mother peripheral blood mononuclear cell priority journal protein expression protein function reverse transcription polymerase chain reaction trophoblast upregulation Western blotting Article B lymphocyte CD4+ T lymphocyte cell line flow cytometry homeostasis immune homeostasis inflammation swan 71 cell line trophoblast trophoblast maternal leukocyte interaction CD4-Positive T-Lymphocytes Coculture Techniques Cytokines Dose-Response Relationship, Drug Female Flow Cytometry Gene Expression Regulation Homeostasis Humans Leukocytes Lipopolysaccharides Nitrites Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I Time Factors Transforming Growth Factor beta Trophoblasts Vasoactive Intestinal Peptide |
| description |
Background/Aims: The maternal-fetal interface is a unique immunological site that generates an adequate microenvironment during pregnancy, recognizing and eliminating infections and tolerating the trophoblast/placenta unit. For that purpose, trophoblast cells display several tolerogenic mechanisms to allow fetal survival, such as production of the neuropeptide vasoactive intestinal peptide (VIP). Here we investigated the contribution of VIP to maintain homeostasis at the maternal-placental interface under lipopolysaccharide (LPS) stimulation. Methods: We performed cocultures between trophoblast cells (Swan-71 cell line) and maternal leukocytes obtained from fertile women as an in vitro model of maternal-placental interaction, and we focused on the effects of LPS on the modulation of VIP and their receptors (VPAC1 and VPAC 2). Results: VIP could prevent the upregulation of IL-6, MCP-1, and nitrite production and maintain the production of IL-10 and TGF-β under LPS (10 μg/ml) stimulation after 48 h of coculture. To gain deeper insight into the mechanisms of how VIP could contribute to a tolerogenic microenvironment even in the presence of LPS, we investigated VIP production by maternal leukocytes and observed a significant increase in the frequency of CD4+VIP+ cells after interaction with Swan-71 cells in the presence of LPS. LPS increased VIP and inducible receptor VPAC2 expression directly on trophoblast cells in a dose- and time-dependent manner. Conclusions: The present results suggest that VIP might act as an additional homeostatic mechanism during early stages at the maternal-placental interface to control exacerbated inflammatory responses such as the ones observed in intrauterine infections. © 2013 S. Karger AG, Basel. |
| format |
JOUR |
| author |
Fraccaroli, L. Grasso, E. Hauk, V. Cortelezzi, M. Pérez Leirós, C. Ramhorst, R. |
| author_facet |
Fraccaroli, L. Grasso, E. Hauk, V. Cortelezzi, M. Pérez Leirós, C. Ramhorst, R. |
| author_sort |
Fraccaroli, L. |
| title |
Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation |
| title_short |
Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation |
| title_full |
Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation |
| title_fullStr |
Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation |
| title_full_unstemmed |
Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation |
| title_sort |
contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under lps stimulation |
| url |
http://hdl.handle.net/20.500.12110/paper_10217401_v21_n1_p21_Fraccaroli |
| work_keys_str_mv |
AT fraccarolil contributionofvasoactiveintestinalpeptidetoimmunehomeostasisintrophoblastmaternalleukocyteinteractionunderlpsstimulation AT grassoe contributionofvasoactiveintestinalpeptidetoimmunehomeostasisintrophoblastmaternalleukocyteinteractionunderlpsstimulation AT haukv contributionofvasoactiveintestinalpeptidetoimmunehomeostasisintrophoblastmaternalleukocyteinteractionunderlpsstimulation AT cortelezzim contributionofvasoactiveintestinalpeptidetoimmunehomeostasisintrophoblastmaternalleukocyteinteractionunderlpsstimulation AT perezleirosc contributionofvasoactiveintestinalpeptidetoimmunehomeostasisintrophoblastmaternalleukocyteinteractionunderlpsstimulation AT ramhorstr contributionofvasoactiveintestinalpeptidetoimmunehomeostasisintrophoblastmaternalleukocyteinteractionunderlpsstimulation |
| _version_ |
1782024941016711168 |