NOD mice exocrinopathy: Towards a neuroimmune link
Sjögren's syndrome (SS) is a chronic autoimmune disorder of exocrine glands characterized as an autoimmune exocrinopathy and more specifically as an autoimmune epithelitis. An impaired balance of neuroimmune interactions mediated by vasoactive intestinal peptide (VIP) in the target organ at ear...
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todo:paper_10217401_v14_n3-4_p175_Calafat2023-10-03T15:56:37Z NOD mice exocrinopathy: Towards a neuroimmune link Calafat, M. Larocca, L. Roca, V. Pérez Leirós, C. Apoptotic acinar cells Exocrinopathy Macrophages Neuroimmune Nitric oxide NOD mice gamma interferon interleukin 10 lipopolysaccharide nitric oxide nitrite vasoactive intestinal polypeptide acinar cell animal cell animal model apoptosis conference paper controlled study female macrophage mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal Sjoegren syndrome stimulation submandibular gland Animals Apoptosis Cells, Cultured Disease Models, Animal Epithelial Cells Epithelium Female Inflammation Mediators Interferon Type II Interleukin-10 Lipopolysaccharides Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Neuroimmunomodulation Nitric Oxide Peritoneum Sjogren's Syndrome Submandibular Gland Vasoactive Intestinal Peptide Sjögren's syndrome (SS) is a chronic autoimmune disorder of exocrine glands characterized as an autoimmune exocrinopathy and more specifically as an autoimmune epithelitis. An impaired balance of neuroimmune interactions mediated by vasoactive intestinal peptide (VIP) in the target organ at early stages of disease is explored by means of the nonobese diabetic (NOD) mouse model of SS. We have previously described a reduced salivary secretion and signaling upon VIP stimulation. The effect reflected a differential regulation of the neural isoform of nitric oxide synthase by calcium calmodulin kinase II and occurred prior to the appearance of detectable levels of cytokines in NOD glands. VIP acting on NOD macrophages treated with lipopolysaccharide promoted anti-inflammatory effects by inhibiting nitric oxide synthase induction as well as IL-12 and TNF-α production, while stimulating IL-10. Here we present evidence on the ability of apoptotic acinar cells from submandibular glands of NOD mice to stimulate nitric oxide in both peritoneal and glandular macrophage pools to a similar extent as lipopolysaccharide + IFN-γ. VIP was not effective to prevent nitrite accumulation and modestly increased IL-10 levels in macrophages coincubated with acinar cells. An enhanced nitrite response of NOD glandular macrophages in basal and stimulated conditions compared to peritoneal cells is also shown. Copyright © 2007 S. Karger AG. Fil:Calafat, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Larocca, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roca, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez Leirós, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10217401_v14_n3-4_p175_Calafat |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptotic acinar cells Exocrinopathy Macrophages Neuroimmune Nitric oxide NOD mice gamma interferon interleukin 10 lipopolysaccharide nitric oxide nitrite vasoactive intestinal polypeptide acinar cell animal cell animal model apoptosis conference paper controlled study female macrophage mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal Sjoegren syndrome stimulation submandibular gland Animals Apoptosis Cells, Cultured Disease Models, Animal Epithelial Cells Epithelium Female Inflammation Mediators Interferon Type II Interleukin-10 Lipopolysaccharides Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Neuroimmunomodulation Nitric Oxide Peritoneum Sjogren's Syndrome Submandibular Gland Vasoactive Intestinal Peptide |
| spellingShingle |
Apoptotic acinar cells Exocrinopathy Macrophages Neuroimmune Nitric oxide NOD mice gamma interferon interleukin 10 lipopolysaccharide nitric oxide nitrite vasoactive intestinal polypeptide acinar cell animal cell animal model apoptosis conference paper controlled study female macrophage mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal Sjoegren syndrome stimulation submandibular gland Animals Apoptosis Cells, Cultured Disease Models, Animal Epithelial Cells Epithelium Female Inflammation Mediators Interferon Type II Interleukin-10 Lipopolysaccharides Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Neuroimmunomodulation Nitric Oxide Peritoneum Sjogren's Syndrome Submandibular Gland Vasoactive Intestinal Peptide Calafat, M. Larocca, L. Roca, V. Pérez Leirós, C. NOD mice exocrinopathy: Towards a neuroimmune link |
| topic_facet |
Apoptotic acinar cells Exocrinopathy Macrophages Neuroimmune Nitric oxide NOD mice gamma interferon interleukin 10 lipopolysaccharide nitric oxide nitrite vasoactive intestinal polypeptide acinar cell animal cell animal model apoptosis conference paper controlled study female macrophage mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal Sjoegren syndrome stimulation submandibular gland Animals Apoptosis Cells, Cultured Disease Models, Animal Epithelial Cells Epithelium Female Inflammation Mediators Interferon Type II Interleukin-10 Lipopolysaccharides Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD Neuroimmunomodulation Nitric Oxide Peritoneum Sjogren's Syndrome Submandibular Gland Vasoactive Intestinal Peptide |
| description |
Sjögren's syndrome (SS) is a chronic autoimmune disorder of exocrine glands characterized as an autoimmune exocrinopathy and more specifically as an autoimmune epithelitis. An impaired balance of neuroimmune interactions mediated by vasoactive intestinal peptide (VIP) in the target organ at early stages of disease is explored by means of the nonobese diabetic (NOD) mouse model of SS. We have previously described a reduced salivary secretion and signaling upon VIP stimulation. The effect reflected a differential regulation of the neural isoform of nitric oxide synthase by calcium calmodulin kinase II and occurred prior to the appearance of detectable levels of cytokines in NOD glands. VIP acting on NOD macrophages treated with lipopolysaccharide promoted anti-inflammatory effects by inhibiting nitric oxide synthase induction as well as IL-12 and TNF-α production, while stimulating IL-10. Here we present evidence on the ability of apoptotic acinar cells from submandibular glands of NOD mice to stimulate nitric oxide in both peritoneal and glandular macrophage pools to a similar extent as lipopolysaccharide + IFN-γ. VIP was not effective to prevent nitrite accumulation and modestly increased IL-10 levels in macrophages coincubated with acinar cells. An enhanced nitrite response of NOD glandular macrophages in basal and stimulated conditions compared to peritoneal cells is also shown. Copyright © 2007 S. Karger AG. |
| format |
JOUR |
| author |
Calafat, M. Larocca, L. Roca, V. Pérez Leirós, C. |
| author_facet |
Calafat, M. Larocca, L. Roca, V. Pérez Leirós, C. |
| author_sort |
Calafat, M. |
| title |
NOD mice exocrinopathy: Towards a neuroimmune link |
| title_short |
NOD mice exocrinopathy: Towards a neuroimmune link |
| title_full |
NOD mice exocrinopathy: Towards a neuroimmune link |
| title_fullStr |
NOD mice exocrinopathy: Towards a neuroimmune link |
| title_full_unstemmed |
NOD mice exocrinopathy: Towards a neuroimmune link |
| title_sort |
nod mice exocrinopathy: towards a neuroimmune link |
| url |
http://hdl.handle.net/20.500.12110/paper_10217401_v14_n3-4_p175_Calafat |
| work_keys_str_mv |
AT calafatm nodmiceexocrinopathytowardsaneuroimmunelink AT laroccal nodmiceexocrinopathytowardsaneuroimmunelink AT rocav nodmiceexocrinopathytowardsaneuroimmunelink AT perezleirosc nodmiceexocrinopathytowardsaneuroimmunelink |
| _version_ |
1807314423307567104 |