Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity

In this work, we studied the in vitro interactions between aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and nitric oxide (NO), as well as the interactions between ALA, porphyrins and some NO donors and precursors. We employed three murine adenocarcinoma cell lines: LM2, which does n...

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Autores principales:	Di Venosa, G., Perotti, C., Fukuda, H., Batlle, A., Casas, A.
Formato:	JOUR
Materias:	5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10111344_v80_n3_p195_DiVenosa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_10111344_v80_n3_p195_DiVenosa2023-10-03T15:56:15Z Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity Di Venosa, G. Perotti, C. Fukuda, H. Batlle, A. Casas, A. 5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae In this work, we studied the in vitro interactions between aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and nitric oxide (NO), as well as the interactions between ALA, porphyrins and some NO donors and precursors. We employed three murine adenocarcinoma cell lines: LM2, which does not produce NO; LM3, which produces NO, and LM3-SNP, a variant of LM3 resistant to NO producing the same amount of NO as the parental. We did not find cross-resistance between NO-induced cytotoxicity and ALA-PDT. In spite of the lower porphyrin synthesis, LM2 cells show the highest sensitivity to ALA-PDT. However, we hypothesised that this is not related to the lack of endogenous NO production, because modulation of NO levels did not modify the response to PDT in any of the cell lines. Two unexpected results were found: the enhancement of NO production from the donor sodium nitroprusside (SNP) induced by ALA in both cells and medium, and the inhibition by ALA of NO production from arginine. We also found that SNP strongly protected the cells from ALA-PDT by impairing porphyrin biosynthesis as a consequence of an inhibition of the enzyme ALA dehydratase. We were not able to evaluate the action of NO derived from SNP because of the unexpected porphyrin impairment. On the other hand, impairment of NO from Arginine driven by ALA, although not modulating in vitro the ALA-PDT response, by increasing in vivo blood flow, may be contributing to the mechanism of tumour cures. © 2005 Elsevier B.V. All rights reserved. Fil:Di Venosa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10111344_v80_n3_p195_DiVenosa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae
spellingShingle	5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae Di Venosa, G. Perotti, C. Fukuda, H. Batlle, A. Casas, A. Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
topic_facet	5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae
description	In this work, we studied the in vitro interactions between aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and nitric oxide (NO), as well as the interactions between ALA, porphyrins and some NO donors and precursors. We employed three murine adenocarcinoma cell lines: LM2, which does not produce NO; LM3, which produces NO, and LM3-SNP, a variant of LM3 resistant to NO producing the same amount of NO as the parental. We did not find cross-resistance between NO-induced cytotoxicity and ALA-PDT. In spite of the lower porphyrin synthesis, LM2 cells show the highest sensitivity to ALA-PDT. However, we hypothesised that this is not related to the lack of endogenous NO production, because modulation of NO levels did not modify the response to PDT in any of the cell lines. Two unexpected results were found: the enhancement of NO production from the donor sodium nitroprusside (SNP) induced by ALA in both cells and medium, and the inhibition by ALA of NO production from arginine. We also found that SNP strongly protected the cells from ALA-PDT by impairing porphyrin biosynthesis as a consequence of an inhibition of the enzyme ALA dehydratase. We were not able to evaluate the action of NO derived from SNP because of the unexpected porphyrin impairment. On the other hand, impairment of NO from Arginine driven by ALA, although not modulating in vitro the ALA-PDT response, by increasing in vivo blood flow, may be contributing to the mechanism of tumour cures. © 2005 Elsevier B.V. All rights reserved.
format	JOUR
author	Di Venosa, G. Perotti, C. Fukuda, H. Batlle, A. Casas, A.
author_facet	Di Venosa, G. Perotti, C. Fukuda, H. Batlle, A. Casas, A.
author_sort	Di Venosa, G.
title	Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
title_short	Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
title_full	Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
title_fullStr	Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
title_full_unstemmed	Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
title_sort	sensitivity to ala-pdt of cell lines with different nitric oxide production and resistance to no cytotoxicity
url	http://hdl.handle.net/20.500.12110/paper_10111344_v80_n3_p195_DiVenosa
work_keys_str_mv	AT divenosag sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT perottic sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT fukudah sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT batllea sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT casasa sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity
_version_	1807321172049657856

Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity

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