Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins
Heparin, a highly sulfated polysaccharide used as an antithrombotic and anticoagulant, inhibits proliferation of several cell types. We have investigated the effect of heparin and chemically modified heparins on the growth of a cell culture of a murine mammary adenocarcinoma (M3). We found that hepa...
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todo:paper_10104283_v15_n5_p275_Bertolesi2023-10-03T15:55:57Z Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins Bertolesi, G.E. De Cidre, L.L. Eiján, A.M. Growth Heparin Heparinoid Inhibition Metastasis Murine adenocarcinoma heparin heparin derivative animal cell animal experiment animal model article breast carcinoma controlled study growth inhibition mouse nonhuman priority journal Adenocarcinoma Animal Cell Division Comparative Study Cytoplasmic Granules Heparin Kinetics Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Time Factors Tumor Cells, Cultured Heparin, a highly sulfated polysaccharide used as an antithrombotic and anticoagulant, inhibits proliferation of several cell types. We have investigated the effect of heparin and chemically modified heparins on the growth of a cell culture of a murine mammary adenocarcinoma (M3). We found that heparin inhibited the proliferation of M3 cells growing either with or without 2% fetal calf serum (FCS) in a dose-dependent and reversible fashion. Several heparins with different anticoagulant properties showed a similar antiproliferative effect. Histological assays showed that heparin was internalized and appeared in cytoplasmic vesicules. O-desulfated. O/N-desul- fated N-acetylated and N-desulfated heparins lost their antiproliferative activity, while N-desulfated N-acetylated heparin significantly inhibited cell proliferation with or without FCS. The finding of an antiproliferative action of N-desulfated N-acetylated heparin which does not show anticoagulant activity suggests a possible therapeutic role for this compound as an antineoplastic drug. © 1994 S. Karger AG, Basel. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10104283_v15_n5_p275_Bertolesi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Growth Heparin Heparinoid Inhibition Metastasis Murine adenocarcinoma heparin heparin derivative animal cell animal experiment animal model article breast carcinoma controlled study growth inhibition mouse nonhuman priority journal Adenocarcinoma Animal Cell Division Comparative Study Cytoplasmic Granules Heparin Kinetics Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Time Factors Tumor Cells, Cultured |
| spellingShingle |
Growth Heparin Heparinoid Inhibition Metastasis Murine adenocarcinoma heparin heparin derivative animal cell animal experiment animal model article breast carcinoma controlled study growth inhibition mouse nonhuman priority journal Adenocarcinoma Animal Cell Division Comparative Study Cytoplasmic Granules Heparin Kinetics Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Time Factors Tumor Cells, Cultured Bertolesi, G.E. De Cidre, L.L. Eiján, A.M. Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins |
| topic_facet |
Growth Heparin Heparinoid Inhibition Metastasis Murine adenocarcinoma heparin heparin derivative animal cell animal experiment animal model article breast carcinoma controlled study growth inhibition mouse nonhuman priority journal Adenocarcinoma Animal Cell Division Comparative Study Cytoplasmic Granules Heparin Kinetics Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Time Factors Tumor Cells, Cultured |
| description |
Heparin, a highly sulfated polysaccharide used as an antithrombotic and anticoagulant, inhibits proliferation of several cell types. We have investigated the effect of heparin and chemically modified heparins on the growth of a cell culture of a murine mammary adenocarcinoma (M3). We found that heparin inhibited the proliferation of M3 cells growing either with or without 2% fetal calf serum (FCS) in a dose-dependent and reversible fashion. Several heparins with different anticoagulant properties showed a similar antiproliferative effect. Histological assays showed that heparin was internalized and appeared in cytoplasmic vesicules. O-desulfated. O/N-desul- fated N-acetylated and N-desulfated heparins lost their antiproliferative activity, while N-desulfated N-acetylated heparin significantly inhibited cell proliferation with or without FCS. The finding of an antiproliferative action of N-desulfated N-acetylated heparin which does not show anticoagulant activity suggests a possible therapeutic role for this compound as an antineoplastic drug. © 1994 S. Karger AG, Basel. |
| format |
JOUR |
| author |
Bertolesi, G.E. De Cidre, L.L. Eiján, A.M. |
| author_facet |
Bertolesi, G.E. De Cidre, L.L. Eiján, A.M. |
| author_sort |
Bertolesi, G.E. |
| title |
Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins |
| title_short |
Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins |
| title_full |
Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins |
| title_fullStr |
Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins |
| title_full_unstemmed |
Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins |
| title_sort |
growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins |
| url |
http://hdl.handle.net/20.500.12110/paper_10104283_v15_n5_p275_Bertolesi |
| work_keys_str_mv |
AT bertolesige growthinhibitioninvitroofmurinemammaryadenocarcinomacellsbyheparinandchemicallymodifiedheparins AT decidrell growthinhibitioninvitroofmurinemammaryadenocarcinomacellsbyheparinandchemicallymodifiedheparins AT eijanam growthinhibitioninvitroofmurinemammaryadenocarcinomacellsbyheparinandchemicallymodifiedheparins |
| _version_ |
1807320139869192192 |