Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)

Fragile sites are points of preferential breakage that may be involved in chromosome rearrangements. Induction of common fragile sites (c-fra) and spontaneous breakage were analyzed in two New World Monkeys species: Saimiri boliviensis (SBO) and Alouatta caraya (ACA). Spontaneous chromosome aberrati...

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Autores principales: Fundia, A., Gorostiaga, M., Mudry, M.
Formato: JOUR
Materias:
Ceboidea
Chromosomal rearrangements
Evolution
Fragile sites
Heterochromatin
article
chromosome breakage
chromosome rearrangement
evolution
gene expression
heterochromatin
monkey
nonhuman
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0999193X_v32_n1_p87_Fundia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0999193X_v32_n1_p87_Fundia
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spelling todo:paper_0999193X_v32_n1_p87_Fundia2023-10-03T15:55:46Z Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini) Fundia, A. Gorostiaga, M. Mudry, M. Ceboidea Chromosomal rearrangements Evolution Fragile sites Heterochromatin article chromosome breakage chromosome rearrangement evolution gene expression heterochromatin monkey nonhuman Fragile sites are points of preferential breakage that may be involved in chromosome rearrangements. Induction of common fragile sites (c-fra) and spontaneous breakage were analyzed in two New World Monkeys species: Saimiri boliviensis (SBO) and Alouatta caraya (ACA). Spontaneous chromosome aberrations were analyzed on untreated lymphocyte cultures with Brogger's formula (1977). SBO presented a low level of spontaneous breakage, while higher frequencies were detected in ACA in which bands 1q23; 2q13 and 11q19 were significantly affected (p < 0.01). The populational distribution of c-fra was analyzed by the Chi2 test in FUdR plus caffeine treated cultures. A total of 21 c-fra was identified in SBO and 24 in ACA. Fragile sites A1q33, B1p21, B4p14, C3q23 and C5q22 were identified in all analyzed SBO specimens. The most frequent c-fra identified in ACA specimens were 1q23, 1q31, 1q33, 2q22, 8q14, 12q31, 13q22, 14q15 and Xq22. Fragile sites A1q31, A1q33, B1q14, B3q13, B4q21 and Xq22 identified in SBO and 1q31, 1q33, 2q22, 4q21, 6q13, 13q22 and Xq22 from ACA were the most conserved sites. A low coincidence between the location of c-fra and that of heterochromatin and breakpoints involved in euchromatic rearrangements known for these genera, was established. Fil:Gorostiaga, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0999193X_v32_n1_p87_Fundia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ceboidea
Chromosomal rearrangements
Evolution
Fragile sites
Heterochromatin
article
chromosome breakage
chromosome rearrangement
evolution
gene expression
heterochromatin
monkey
nonhuman
spellingShingle Ceboidea
Chromosomal rearrangements
Evolution
Fragile sites
Heterochromatin
article
chromosome breakage
chromosome rearrangement
evolution
gene expression
heterochromatin
monkey
nonhuman
Fundia, A.
Gorostiaga, M.
Mudry, M.
Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)
topic_facet Ceboidea
Chromosomal rearrangements
Evolution
Fragile sites
Heterochromatin
article
chromosome breakage
chromosome rearrangement
evolution
gene expression
heterochromatin
monkey
nonhuman
description Fragile sites are points of preferential breakage that may be involved in chromosome rearrangements. Induction of common fragile sites (c-fra) and spontaneous breakage were analyzed in two New World Monkeys species: Saimiri boliviensis (SBO) and Alouatta caraya (ACA). Spontaneous chromosome aberrations were analyzed on untreated lymphocyte cultures with Brogger's formula (1977). SBO presented a low level of spontaneous breakage, while higher frequencies were detected in ACA in which bands 1q23; 2q13 and 11q19 were significantly affected (p < 0.01). The populational distribution of c-fra was analyzed by the Chi2 test in FUdR plus caffeine treated cultures. A total of 21 c-fra was identified in SBO and 24 in ACA. Fragile sites A1q33, B1p21, B4p14, C3q23 and C5q22 were identified in all analyzed SBO specimens. The most frequent c-fra identified in ACA specimens were 1q23, 1q31, 1q33, 2q22, 8q14, 12q31, 13q22, 14q15 and Xq22. Fragile sites A1q31, A1q33, B1q14, B3q13, B4q21 and Xq22 identified in SBO and 1q31, 1q33, 2q22, 4q21, 6q13, 13q22 and Xq22 from ACA were the most conserved sites. A low coincidence between the location of c-fra and that of heterochromatin and breakpoints involved in euchromatic rearrangements known for these genera, was established.
format JOUR
author Fundia, A.
Gorostiaga, M.
Mudry, M.
author_facet Fundia, A.
Gorostiaga, M.
Mudry, M.
author_sort Fundia, A.
title Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)
title_short Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)
title_full Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)
title_fullStr Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)
title_full_unstemmed Expression of common fragile sites in two ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)
title_sort expression of common fragile sites in two ceboidea species: saimiri boliviensis and alouatta caraya (primates: platyrrhini)
url http://hdl.handle.net/20.500.12110/paper_0999193X_v32_n1_p87_Fundia
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AT gorostiagam expressionofcommonfragilesitesintwoceboideaspeciessaimiriboliviensisandalouattacarayaprimatesplatyrrhini
AT mudrym expressionofcommonfragilesitesintwoceboideaspeciessaimiriboliviensisandalouattacarayaprimatesplatyrrhini
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