Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)

Different types of voltage-dependent calcium channels (VDCCs) have been recognized based on their molecular structure as well as their pharmacological and biophysical properties. One of these, the P/Q type, is the main channel involved in nerve evoked neurotransmitter release at neuromuscular juncti...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Urbano, F.J., Rosato-Siri, M.D., Uchitel, O.D.
Formato: JOUR
Materias:
Bapta
Calcium channels
Development
Egta
Neuromuscular junction
calcium channel N type
calcium channel P type
calcium channel Q type
calcium channel R type
calcium ion
egtazic acid
ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid
neurotransmitter
animal cell
controlled study
knockout mouse
mouse
neuromuscular synapse
neuromuscular transmission
neurotransmitter release
newborn
nonhuman
priority journal
protein expression
rat
review
Animals
Calcium Channels
Calcium Channels, L-Type
Mice
Neuromuscular Junction
Neuromuscular Junction Diseases
Neurotransmitter Agents
Rats
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09687688_v19_n4_p293_Urbano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09687688_v19_n4_p293_Urbano
record_format dspace
spelling todo:paper_09687688_v19_n4_p293_Urbano2023-10-03T15:55:21Z Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review) Urbano, F.J. Rosato-Siri, M.D. Uchitel, O.D. Bapta Calcium channels Development Egta Neuromuscular junction calcium channel N type calcium channel P type calcium channel Q type calcium channel R type calcium ion egtazic acid ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid neurotransmitter animal cell controlled study knockout mouse mouse neuromuscular synapse neuromuscular transmission neurotransmitter release newborn nonhuman priority journal protein expression rat review Animals Calcium Channels Calcium Channels, L-Type Mice Neuromuscular Junction Neuromuscular Junction Diseases Neurotransmitter Agents Rats Animalia Different types of voltage-dependent calcium channels (VDCCs) have been recognized based on their molecular structure as well as their pharmacological and biophysical properties. One of these, the P/Q type, is the main channel involved in nerve evoked neurotransmitter release at neuromuscular junctions (NMJs) and many central nervous system synapses. However, under particular experimental or biological conditions, other channels can be involved. L-type VDCC presence at the NMJ has been demonstrated by the contribution to the perineural calcium currents (ICa) at adult mice Bapta-loaded NMJs. This is probably a result of a reduction in Ca2+ inactivation. The L-type current was not coupled to neurotransmitter release, but became coupled, as demonstrated by the release of acetylcholine, after the inhibition of serine/threonine protein phosphatases with okadaic acid (OA). Thus, under these conditions, L-type channels were unmasked at Bapta- but not at Egta-loaded NMJs. This suggests that the speed, not the capacity, of the calcium chelator was decisive in preventing Ca2+-inactivation and facilitating the contribution to neurotransmitter release. At neonatal rat NMJs, N-type VDCCs were involved early during development whereas P/Q-type VDCCs play a main role at all stages of development. Furthermore, P/Q-type VDCCs were more efficiently coupled to neurotransmitter release than N-type VDCCs. This difference could be accounted for by a differential location of these channels at the release site. Neuromuscular transmission in P/Q-type calcium channel knock out ataxic mice jointly depends on both N-type and R-type channels and shows several altered properties including low quantal content. Thus, calcium channels may be recruited to mediate neurotransmitter release with a functional hierarchy where the P/Q channel seems to be the channel most suited to mediate exocytosis at NMJs. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09687688_v19_n4_p293_Urbano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bapta
Calcium channels
Development
Egta
Neuromuscular junction
calcium channel N type
calcium channel P type
calcium channel Q type
calcium channel R type
calcium ion
egtazic acid
ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid
neurotransmitter
animal cell
controlled study
knockout mouse
mouse
neuromuscular synapse
neuromuscular transmission
neurotransmitter release
newborn
nonhuman
priority journal
protein expression
rat
review
Animals
Calcium Channels
Calcium Channels, L-Type
Mice
Neuromuscular Junction
Neuromuscular Junction Diseases
Neurotransmitter Agents
Rats
Animalia
spellingShingle Bapta
Calcium channels
Development
Egta
Neuromuscular junction
calcium channel N type
calcium channel P type
calcium channel Q type
calcium channel R type
calcium ion
egtazic acid
ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid
neurotransmitter
animal cell
controlled study
knockout mouse
mouse
neuromuscular synapse
neuromuscular transmission
neurotransmitter release
newborn
nonhuman
priority journal
protein expression
rat
review
Animals
Calcium Channels
Calcium Channels, L-Type
Mice
Neuromuscular Junction
Neuromuscular Junction Diseases
Neurotransmitter Agents
Rats
Animalia
Urbano, F.J.
Rosato-Siri, M.D.
Uchitel, O.D.
Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)
topic_facet Bapta
Calcium channels
Development
Egta
Neuromuscular junction
calcium channel N type
calcium channel P type
calcium channel Q type
calcium channel R type
calcium ion
egtazic acid
ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid
neurotransmitter
animal cell
controlled study
knockout mouse
mouse
neuromuscular synapse
neuromuscular transmission
neurotransmitter release
newborn
nonhuman
priority journal
protein expression
rat
review
Animals
Calcium Channels
Calcium Channels, L-Type
Mice
Neuromuscular Junction
Neuromuscular Junction Diseases
Neurotransmitter Agents
Rats
Animalia
description Different types of voltage-dependent calcium channels (VDCCs) have been recognized based on their molecular structure as well as their pharmacological and biophysical properties. One of these, the P/Q type, is the main channel involved in nerve evoked neurotransmitter release at neuromuscular junctions (NMJs) and many central nervous system synapses. However, under particular experimental or biological conditions, other channels can be involved. L-type VDCC presence at the NMJ has been demonstrated by the contribution to the perineural calcium currents (ICa) at adult mice Bapta-loaded NMJs. This is probably a result of a reduction in Ca2+ inactivation. The L-type current was not coupled to neurotransmitter release, but became coupled, as demonstrated by the release of acetylcholine, after the inhibition of serine/threonine protein phosphatases with okadaic acid (OA). Thus, under these conditions, L-type channels were unmasked at Bapta- but not at Egta-loaded NMJs. This suggests that the speed, not the capacity, of the calcium chelator was decisive in preventing Ca2+-inactivation and facilitating the contribution to neurotransmitter release. At neonatal rat NMJs, N-type VDCCs were involved early during development whereas P/Q-type VDCCs play a main role at all stages of development. Furthermore, P/Q-type VDCCs were more efficiently coupled to neurotransmitter release than N-type VDCCs. This difference could be accounted for by a differential location of these channels at the release site. Neuromuscular transmission in P/Q-type calcium channel knock out ataxic mice jointly depends on both N-type and R-type channels and shows several altered properties including low quantal content. Thus, calcium channels may be recruited to mediate neurotransmitter release with a functional hierarchy where the P/Q channel seems to be the channel most suited to mediate exocytosis at NMJs.
format JOUR
author Urbano, F.J.
Rosato-Siri, M.D.
Uchitel, O.D.
author_facet Urbano, F.J.
Rosato-Siri, M.D.
Uchitel, O.D.
author_sort Urbano, F.J.
title Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)
title_short Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)
title_full Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)
title_fullStr Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)
title_full_unstemmed Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)
title_sort calcium channels involved in neurotransmitter release at adult, neonatal and p/q-type deficient neuromuscular junctions (review)
url http://hdl.handle.net/20.500.12110/paper_09687688_v19_n4_p293_Urbano
work_keys_str_mv AT urbanofj calciumchannelsinvolvedinneurotransmitterreleaseatadultneonatalandpqtypedeficientneuromuscularjunctionsreview
AT rosatosirimd calciumchannelsinvolvedinneurotransmitterreleaseatadultneonatalandpqtypedeficientneuromuscularjunctionsreview
AT uchitelod calciumchannelsinvolvedinneurotransmitterreleaseatadultneonatalandpqtypedeficientneuromuscularjunctionsreview
_version_ 1807320662273949696

Ejemplares similares