Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea)
This study analyzes the dynamics of the vitellogenesis process in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides, based on light microscopic observations. The ovotestes of the shrimps at the different gonadal development stages were removed, fixed and submitted an usua...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09684328_v88_n_p60_Braga |
| Aporte de: |
| id |
todo:paper_09684328_v88_n_p60_Braga |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_09684328_v88_n_p60_Braga2023-10-03T15:55:21Z Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea) Braga, A.A. Nunes, E.T. López-Grecco, L.S. Camargo-Mathias, M.I. Fransozo, V. Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Carbohydrates Cytology Proteins Shellfish Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Cells animal classification cytochemistry cytology Decapoda (Crustacea) disorder of sex development female germ cell gonad growth, development and aging hermaphrodite histology male microscopy oocyte oocyte development ovary physiology ultrastructure Animals Decapoda (Crustacea) Disorders of Sex Development Female Germ Cells Gonads Hermaphroditic Organisms Histocytochemistry Histological Techniques Histology Male Microscopy Oocytes Oogenesis Ovary This study analyzes the dynamics of the vitellogenesis process in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides, based on light microscopic observations. The ovotestes of the shrimps at the different gonadal development stages were removed, fixed and submitted an usual histological procedure (HE) and histochemical techniques (Bromophenol Blue, PAS/Alcian Blue, and Von Kossa tests). The germinative cells were classified into oogonias, and oocytes in stages I–IV based on the following features: cell size, cytoplasm appearance, presence of yolk granules, lipid droplets, chorion, and chromatin patterns. In the male initial phase of the gonadal development, the ovotestes present mainly oogonia and oocytes I and II while in the functional hermaphrodite phase, oocytes III and IV predominate in the peripheral zone of the gonads. Oocytes with an atypical appearance of the cellular components indicative of reabsorption were also observed. This study showed an increasing accumulation of proteins, carbohydrates and lipids occurring as the germ cells develop, being the yolk elements deposited in a sequence, in which proteins and carbohydrates are the first to appear both by an endogenous as well as also by an exogenous origin. The presence of calcium was detected mainly in oocytes I, II and inside those in reabsorption, being posteriorly mobilized to chorion constitution and/or to hemolymph due to its role during molting. Although the similarity of the germ cells shape among the crustaceans, this first histochemical characterization of E. oplophoproides ovary increases the comprehension of oogenesis in a caridean simultaneous protandric hermaphrodite species. © 2016 Elsevier Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09684328_v88_n_p60_Braga |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Carbohydrates Cytology Proteins Shellfish Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Cells animal classification cytochemistry cytology Decapoda (Crustacea) disorder of sex development female germ cell gonad growth, development and aging hermaphrodite histology male microscopy oocyte oocyte development ovary physiology ultrastructure Animals Decapoda (Crustacea) Disorders of Sex Development Female Germ Cells Gonads Hermaphroditic Organisms Histocytochemistry Histological Techniques Histology Male Microscopy Oocytes Oogenesis Ovary |
| spellingShingle |
Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Carbohydrates Cytology Proteins Shellfish Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Cells animal classification cytochemistry cytology Decapoda (Crustacea) disorder of sex development female germ cell gonad growth, development and aging hermaphrodite histology male microscopy oocyte oocyte development ovary physiology ultrastructure Animals Decapoda (Crustacea) Disorders of Sex Development Female Germ Cells Gonads Hermaphroditic Organisms Histocytochemistry Histological Techniques Histology Male Microscopy Oocytes Oogenesis Ovary Braga, A.A. Nunes, E.T. López-Grecco, L.S. Camargo-Mathias, M.I. Fransozo, V. Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea) |
| topic_facet |
Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Carbohydrates Cytology Proteins Shellfish Hermaphroditism Ovarian development Ovotestes Spine-shrimp Vitellogenesis Cells animal classification cytochemistry cytology Decapoda (Crustacea) disorder of sex development female germ cell gonad growth, development and aging hermaphrodite histology male microscopy oocyte oocyte development ovary physiology ultrastructure Animals Decapoda (Crustacea) Disorders of Sex Development Female Germ Cells Gonads Hermaphroditic Organisms Histocytochemistry Histological Techniques Histology Male Microscopy Oocytes Oogenesis Ovary |
| description |
This study analyzes the dynamics of the vitellogenesis process in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides, based on light microscopic observations. The ovotestes of the shrimps at the different gonadal development stages were removed, fixed and submitted an usual histological procedure (HE) and histochemical techniques (Bromophenol Blue, PAS/Alcian Blue, and Von Kossa tests). The germinative cells were classified into oogonias, and oocytes in stages I–IV based on the following features: cell size, cytoplasm appearance, presence of yolk granules, lipid droplets, chorion, and chromatin patterns. In the male initial phase of the gonadal development, the ovotestes present mainly oogonia and oocytes I and II while in the functional hermaphrodite phase, oocytes III and IV predominate in the peripheral zone of the gonads. Oocytes with an atypical appearance of the cellular components indicative of reabsorption were also observed. This study showed an increasing accumulation of proteins, carbohydrates and lipids occurring as the germ cells develop, being the yolk elements deposited in a sequence, in which proteins and carbohydrates are the first to appear both by an endogenous as well as also by an exogenous origin. The presence of calcium was detected mainly in oocytes I, II and inside those in reabsorption, being posteriorly mobilized to chorion constitution and/or to hemolymph due to its role during molting. Although the similarity of the germ cells shape among the crustaceans, this first histochemical characterization of E. oplophoproides ovary increases the comprehension of oogenesis in a caridean simultaneous protandric hermaphrodite species. © 2016 Elsevier Ltd |
| format |
JOUR |
| author |
Braga, A.A. Nunes, E.T. López-Grecco, L.S. Camargo-Mathias, M.I. Fransozo, V. |
| author_facet |
Braga, A.A. Nunes, E.T. López-Grecco, L.S. Camargo-Mathias, M.I. Fransozo, V. |
| author_sort |
Braga, A.A. |
| title |
Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea) |
| title_short |
Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea) |
| title_full |
Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea) |
| title_fullStr |
Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea) |
| title_full_unstemmed |
Histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp Exhippolysmata oplophoroides (Decapoda: Caridea) |
| title_sort |
histological and histochemical features of the oogenesis in the simultaneous protandric hermaphrodite shrimp exhippolysmata oplophoroides (decapoda: caridea) |
| url |
http://hdl.handle.net/20.500.12110/paper_09684328_v88_n_p60_Braga |
| work_keys_str_mv |
AT bragaaa histologicalandhistochemicalfeaturesoftheoogenesisinthesimultaneousprotandrichermaphroditeshrimpexhippolysmataoplophoroidesdecapodacaridea AT nuneset histologicalandhistochemicalfeaturesoftheoogenesisinthesimultaneousprotandrichermaphroditeshrimpexhippolysmataoplophoroidesdecapodacaridea AT lopezgreccols histologicalandhistochemicalfeaturesoftheoogenesisinthesimultaneousprotandrichermaphroditeshrimpexhippolysmataoplophoroidesdecapodacaridea AT camargomathiasmi histologicalandhistochemicalfeaturesoftheoogenesisinthesimultaneousprotandrichermaphroditeshrimpexhippolysmataoplophoroidesdecapodacaridea AT fransozov histologicalandhistochemicalfeaturesoftheoogenesisinthesimultaneousprotandrichermaphroditeshrimpexhippolysmataoplophoroidesdecapodacaridea |
| _version_ |
1782027630109786112 |