Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation
In oil contaminated environments the poor carbon source availability, the presence of toxic compounds like benzene, toluene, and xylene (BTX), and other biotic and abiotic stresses can reduce bacteria viability and consequently, xenobiotic biodegradation. Selection of bacteria with the capability to...
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todo:paper_09648305_v67_n_p15_DiMartino2023-10-03T15:54:54Z Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation Di Martino, C. López, N.I. Raiger Iustman, L.J. Biosurfactant BTEX Oil bioremediation agent Polyhydroxyalkanoates Pseudomonas Bio surfactant Bioremediation agent BTEX Polyhydroxyalkanoates Pseudomonas Benzene Biodegradation Biomolecules Biopolymers Bioremediation Biotechnology Degradation Genes Hydrocarbons Petroleum refineries Pollution Surface active agents Toluene Wastewater Xylene Bacteria bacterium benzene biodegradation bioremediation concentration (composition) ester growth rate industrial waste microbial activity polymer surfactant toluene wastewater xylene Bacteria (microorganisms) Pseudomonas Pseudomonas sp. In oil contaminated environments the poor carbon source availability, the presence of toxic compounds like benzene, toluene, and xylene (BTX), and other biotic and abiotic stresses can reduce bacteria viability and consequently, xenobiotic biodegradation. Selection of bacteria with the capability to tolerate and degrade monoaromatic compounds, synthesize biosurfactants and accumulate biopolymers that enhance stress tolerance could be a good approach to find a suitable bioaugmentation agent. In this work two Pseudomonas strains were isolated from an oil refinery wastewater based on their capabilities to grow using several hydrocarbons as sole carbon source and to accumulate polyhydroxyalkanoates. Both strains were able to synthesize rhamnolipids as surfactant compounds. One of these isolates, Pseudomonas sp. KA, was able to degrade benzene, toluene, and xylene, and to tolerate them at high concentrations. A molecular screening of the key genes involved in BTX degradation showed that both strains resulted positive for xylA and xylE genes. Pseudomonas sp. KA was also positive for todC1. The presence of both TOL and TOD degradation pathways, a rare characteristic in Pseudomonas species, could be useful for bioremediation purposes. © 2011 Elsevier Ltd. Fil:Raiger Iustman, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09648305_v67_n_p15_DiMartino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biosurfactant BTEX Oil bioremediation agent Polyhydroxyalkanoates Pseudomonas Bio surfactant Bioremediation agent BTEX Polyhydroxyalkanoates Pseudomonas Benzene Biodegradation Biomolecules Biopolymers Bioremediation Biotechnology Degradation Genes Hydrocarbons Petroleum refineries Pollution Surface active agents Toluene Wastewater Xylene Bacteria bacterium benzene biodegradation bioremediation concentration (composition) ester growth rate industrial waste microbial activity polymer surfactant toluene wastewater xylene Bacteria (microorganisms) Pseudomonas Pseudomonas sp. |
| spellingShingle |
Biosurfactant BTEX Oil bioremediation agent Polyhydroxyalkanoates Pseudomonas Bio surfactant Bioremediation agent BTEX Polyhydroxyalkanoates Pseudomonas Benzene Biodegradation Biomolecules Biopolymers Bioremediation Biotechnology Degradation Genes Hydrocarbons Petroleum refineries Pollution Surface active agents Toluene Wastewater Xylene Bacteria bacterium benzene biodegradation bioremediation concentration (composition) ester growth rate industrial waste microbial activity polymer surfactant toluene wastewater xylene Bacteria (microorganisms) Pseudomonas Pseudomonas sp. Di Martino, C. López, N.I. Raiger Iustman, L.J. Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation |
| topic_facet |
Biosurfactant BTEX Oil bioremediation agent Polyhydroxyalkanoates Pseudomonas Bio surfactant Bioremediation agent BTEX Polyhydroxyalkanoates Pseudomonas Benzene Biodegradation Biomolecules Biopolymers Bioremediation Biotechnology Degradation Genes Hydrocarbons Petroleum refineries Pollution Surface active agents Toluene Wastewater Xylene Bacteria bacterium benzene biodegradation bioremediation concentration (composition) ester growth rate industrial waste microbial activity polymer surfactant toluene wastewater xylene Bacteria (microorganisms) Pseudomonas Pseudomonas sp. |
| description |
In oil contaminated environments the poor carbon source availability, the presence of toxic compounds like benzene, toluene, and xylene (BTX), and other biotic and abiotic stresses can reduce bacteria viability and consequently, xenobiotic biodegradation. Selection of bacteria with the capability to tolerate and degrade monoaromatic compounds, synthesize biosurfactants and accumulate biopolymers that enhance stress tolerance could be a good approach to find a suitable bioaugmentation agent. In this work two Pseudomonas strains were isolated from an oil refinery wastewater based on their capabilities to grow using several hydrocarbons as sole carbon source and to accumulate polyhydroxyalkanoates. Both strains were able to synthesize rhamnolipids as surfactant compounds. One of these isolates, Pseudomonas sp. KA, was able to degrade benzene, toluene, and xylene, and to tolerate them at high concentrations. A molecular screening of the key genes involved in BTX degradation showed that both strains resulted positive for xylA and xylE genes. Pseudomonas sp. KA was also positive for todC1. The presence of both TOL and TOD degradation pathways, a rare characteristic in Pseudomonas species, could be useful for bioremediation purposes. © 2011 Elsevier Ltd. |
| format |
JOUR |
| author |
Di Martino, C. López, N.I. Raiger Iustman, L.J. |
| author_facet |
Di Martino, C. López, N.I. Raiger Iustman, L.J. |
| author_sort |
Di Martino, C. |
| title |
Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation |
| title_short |
Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation |
| title_full |
Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation |
| title_fullStr |
Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation |
| title_full_unstemmed |
Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation |
| title_sort |
isolation and characterization of benzene, toluene and xylene degrading pseudomonas sp. selected as candidates for bioremediation |
| url |
http://hdl.handle.net/20.500.12110/paper_09648305_v67_n_p15_DiMartino |
| work_keys_str_mv |
AT dimartinoc isolationandcharacterizationofbenzenetolueneandxylenedegradingpseudomonasspselectedascandidatesforbioremediation AT lopezni isolationandcharacterizationofbenzenetolueneandxylenedegradingpseudomonasspselectedascandidatesforbioremediation AT raigeriustmanlj isolationandcharacterizationofbenzenetolueneandxylenedegradingpseudomonasspselectedascandidatesforbioremediation |
| _version_ |
1782024220211937280 |