Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields

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Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated. The conditions of the PEF treatment were: electric field level of 8-11 kV/cm, 3 pulses, 50 μF capacitance or 12.5 kV/cm, 40 pulses, 1 μF capacitance. The i...

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Autores principales: Molinari, P., Pilosof, A.M.R., Jagus, R.J.
Formato: JOUR
Materias:
Concentration
Pulsed electric field
Yeast
Capacitance
Electric fields
Fruit juices
growth phases
Inoculum size
Food products
Saccharomyces
Saccharomyces cerevisiae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09639969_v37_n8_p793_Molinari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09639969_v37_n8_p793_Molinari
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spelling todo:paper_09639969_v37_n8_p793_Molinari2023-10-03T15:54:38Z Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields Molinari, P. Pilosof, A.M.R. Jagus, R.J. Concentration Pulsed electric field Yeast Capacitance Electric fields Fruit juices Yeast growth phases Inoculum size Food products Saccharomyces Saccharomyces cerevisiae Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated. The conditions of the PEF treatment were: electric field level of 8-11 kV/cm, 3 pulses, 50 μF capacitance or 12.5 kV/cm, 40 pulses, 1 μF capacitance. The inactivation of yeasts ranged from 2 to 5.8 log cycles when the inoculum size increased from 5×105 to 1×1011 CFU/ml, respectively. In all the experiments, the reduction of yeasts population was linearly enhanced by increasing the inoculum size, independently of the growth phase. Different experiments were designed to explain the observed behavior. By increasing yeasts concentration the probability of formation of pearl chains would increase. As a consequence of the large volume of these "equivalent cells" the PEF treatment efficiency would increase. © 2004 Elsevier Ltd. All rights reserved. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jagus, R.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09639969_v37_n8_p793_Molinari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Concentration
Pulsed electric field
Yeast
Capacitance
Electric fields
Fruit juices
Yeast
growth phases
Inoculum size
Food products
Saccharomyces
Saccharomyces cerevisiae
spellingShingle Concentration
Pulsed electric field
Yeast
Capacitance
Electric fields
Fruit juices
Yeast
growth phases
Inoculum size
Food products
Saccharomyces
Saccharomyces cerevisiae
Molinari, P.
Pilosof, A.M.R.
Jagus, R.J.
Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
topic_facet Concentration
Pulsed electric field
Yeast
Capacitance
Electric fields
Fruit juices
Yeast
growth phases
Inoculum size
Food products
Saccharomyces
Saccharomyces cerevisiae
description Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated. The conditions of the PEF treatment were: electric field level of 8-11 kV/cm, 3 pulses, 50 μF capacitance or 12.5 kV/cm, 40 pulses, 1 μF capacitance. The inactivation of yeasts ranged from 2 to 5.8 log cycles when the inoculum size increased from 5×105 to 1×1011 CFU/ml, respectively. In all the experiments, the reduction of yeasts population was linearly enhanced by increasing the inoculum size, independently of the growth phase. Different experiments were designed to explain the observed behavior. By increasing yeasts concentration the probability of formation of pearl chains would increase. As a consequence of the large volume of these "equivalent cells" the PEF treatment efficiency would increase. © 2004 Elsevier Ltd. All rights reserved.
format JOUR
author Molinari, P.
Pilosof, A.M.R.
Jagus, R.J.
author_facet Molinari, P.
Pilosof, A.M.R.
Jagus, R.J.
author_sort Molinari, P.
title Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
title_short Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
title_full Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
title_fullStr Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
title_full_unstemmed Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
title_sort effect of growth phase and inoculum size on the inactivation of saccharomyces cerevisiae in fruit juices, by pulsed electric fields
url http://hdl.handle.net/20.500.12110/paper_09639969_v37_n8_p793_Molinari
work_keys_str_mv AT molinarip effectofgrowthphaseandinoculumsizeontheinactivationofsaccharomycescerevisiaeinfruitjuicesbypulsedelectricfields
AT pilosofamr effectofgrowthphaseandinoculumsizeontheinactivationofsaccharomycescerevisiaeinfruitjuicesbypulsedelectricfields
AT jagusrj effectofgrowthphaseandinoculumsizeontheinactivationofsaccharomycescerevisiaeinfruitjuicesbypulsedelectricfields
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