Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis

The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V., Iribarren, A.M.
Formato:	JOUR
Materias:	Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09636048_v6_n4_p376_Trelles
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09636048_v6_n4_p376_Trelles
record_format	dspace
spelling	todo:paper_09636048_v6_n4_p376_Trelles2023-10-03T15:54:24Z Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis Trelles, J.A. Lewkowicz, E.S. Sinisterra, J.V. Iribarren, A.M. Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use of Citrobacter amalonaticus CECT 863 cells - free or immobilised - in the synthesis of some modified nucleosides. The main characteristics of the immobilised biocatalysts such as type and concentration of polymer, mechanical and storage stability and reuse were assessed. From these studies, polyacrylamide was selected as the best support based on its performance and potential industrial applications. In particular, it can be reused more than 50 times without significant loss of activity. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09636048_v6_n4_p376_Trelles
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus
spellingShingle	Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus Trelles, J.A. Lewkowicz, E.S. Sinisterra, J.V. Iribarren, A.M. Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
topic_facet	Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus
description	The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use of Citrobacter amalonaticus CECT 863 cells - free or immobilised - in the synthesis of some modified nucleosides. The main characteristics of the immobilised biocatalysts such as type and concentration of polymer, mechanical and storage stability and reuse were assessed. From these studies, polyacrylamide was selected as the best support based on its performance and potential industrial applications. In particular, it can be reused more than 50 times without significant loss of activity.
format	JOUR
author	Trelles, J.A. Lewkowicz, E.S. Sinisterra, J.V. Iribarren, A.M.
author_facet	Trelles, J.A. Lewkowicz, E.S. Sinisterra, J.V. Iribarren, A.M.
author_sort	Trelles, J.A.
title	Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
title_short	Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
title_full	Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
title_fullStr	Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
title_full_unstemmed	Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
title_sort	free and immobilised citrobacter amalonaticus cect 863 as a biocatalyst for nucleoside synthesis
url	http://hdl.handle.net/20.500.12110/paper_09636048_v6_n4_p376_Trelles
work_keys_str_mv	AT trellesja freeandimmobilisedcitrobacteramalonaticuscect863asabiocatalystfornucleosidesynthesis AT lewkowiczes freeandimmobilisedcitrobacteramalonaticuscect863asabiocatalystfornucleosidesynthesis AT sinisterrajv freeandimmobilisedcitrobacteramalonaticuscect863asabiocatalystfornucleosidesynthesis AT iribarrenam freeandimmobilisedcitrobacteramalonaticuscect863asabiocatalystfornucleosidesynthesis
_version_	1807316360783462400

Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis

Ejemplares similares