2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor

A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthe...

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Detalles Bibliográficos
Autores principales: Pontiggia, R., Pontiggia, O., Simian, M., Montserrat, J.M., Engels, J.W., Iribarren, A.M.
Formato: JOUR
Materias:
2′-C-methyluridine
Estrogen receptor alpha
Modified ribozyme
estrogen receptor alpha
hammerhead ribozyme
hormone receptor blocking agent
hydroxyl group
phosphoramidous acid derivative
article
cell strain MCF 7
chemical modification
controlled study
drug stability
drug synthesis
enzyme active site
genetic transfection
receptor down regulation
Cell Line, Tumor
Humans
Oligonucleotides
Receptors, Estrogen
RNA, Catalytic
RNA, Messenger
Uridine
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n9_p2806_Pontiggia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented. © 2010 Elsevier Ltd. All rights reserved.

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